##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI BfrI BsiWI BsmBI
BsmI BspDI BspLU11I Bst1107I BstBI
BstEII BstPI BstZ17I ClaI Eam1105I
EcoO65I EcoRV EcoT22I HindIII HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI SalI SfuI
SgrAI SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 679 TCCGGA T^CCGGA
Asp700I 1586 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 715 GCACCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1024 TGATCA T^GATCA
BglII 1162 AGATCT A^GATCT
BlpI 1280 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1280 GCTGAGC GC^T[ACGT]AGC
BsaBI 2030 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 679 TCCGGA T^CCGGA
BspEI 679 TCCGGA T^CCGGA
CelII 1280 GCTGAGC GC^T[ACGT]AGC
CpoI 84 CGGACCG CG^G[AT]CCG
DrdI 446 GACGGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1024 TGATCA T^GATCA
HincII 1464 GTTGAC GT[CT]^[GA]AC
HindII 1464 GTTGAC GT[CT]^[GA]AC
MamI 2030 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 679 TCCGGA T^CCGGA
RsrII 84 CGGACCG CG^G[AT]CCG
ScaI 535 AGTACT AGT^ACT
SexAI 3235 ACCAGGT A^CC[AT]GGT
SphI 3556 GCATGC GCATG^C
SspI 4046 AATATT AAT^ATT
XbaI 3933 TCTAGA T^CTAGA
XmnI 1586 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Alw44I 1136 GTGCAC G^TGCAC
4267 GTGCAC
Aor51HI 1201 AGCGCT AGC^GCT
1412 AGCGCT
ApaLI 1136 GTGCAC G^TGCAC
4267 GTGCAC
AvrII 2978 CCTAGG C^CTAGG
3640 CCTAGG
BamHI 982 GGATCC G^GATCC
2662 GGATCC
BbvCI 3532 CCTCAGC CCTCAGC(-5/-2)
4827 GCTGAGG
BciVI 5523 GGATAC GTATCC(6/5)
2815 GTATCC
BlnI 2978 CCTAGG C^CTAGG
3640 CCTAGG
Bsp1407I 802 TGTACA T^GTACA
3831 TGTACA
BsrDI 3793 GCAATG GCAATG(2/0)
5061 GCAATG
BsrGI 802 TGTACA T^GTACA
3831 TGTACA
BssSI 3116 CACGAG CACGAG(-5/-1)
3593 CTCGTG
BstXI 493 CCATGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5490 CCAGTGCCCTGG
Eco47III 1201 AGCGCT AGC^GCT
1412 AGCGCT
EcoRI 1017 GAATTC G^AATTC
3582 GAATTC
NspI 757 GCATGT [GA]CATG^[CT]
3556 GCATGC
SbfI 2510 CCTGCAGG CCTGCA^GG
3473 CCTGCAGG
SfiI 450 GGCCAGTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4547 GGCCTTCGGGGCC
Sse8387I 2510 CCTGCAGG CCTGCA^GG
3473 CCTGCAGG
SspBI 802 TGTACA T^GTACA
3831 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII AviII BalI BanI BanII
BbeI BbrPI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FseI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RcaI
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI