##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspHI AviII AvrII BbeI
BbrPI BcgI BciVI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BsrBI BsrFI BssSI CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRV FseI FspI Hin1I HpaI
KasI KspI MamI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 63 GGTACC G^GTACC
AccII 3656 CGCG CG^CG
AclI 1861 AACGTT AA^CGTT
AflII 2855 CTTAAG C^TTAAG
AlwNI 2197 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 63 GGTACC G^GTACC
AspI 141 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3288 CCCGGG C^[CT]CG[GA]G
BamHI 3904 GGATCC G^GATCC
BanII 3267 GAGCCC G[GA]GC[CT]^C
BbsI 1445 GAAGAC GAAGAC(2/6)
BclI 1054 TGATCA T^GATCA
BfrI 2855 CTTAAG C^TTAAG
BpmI 3414 CTGGAG CTGGAG(16/14)
BpuAI 1445 GAAGAC GAAGAC(2/6)
BsaWI 66 ACCGGA [AT]^CCGG[AT]
BsoBI 3288 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3702 TGTACA T^GTACA
BspHI 4425 TCATGA T^CATGA
BsrGI 3702 TGTACA T^GTACA
BssHII 3655 GCGCGC G^CGCGC
BstAPI 1364 GCAGTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4214 TTCGAA TT^CGAA
BstEII 124 GGTTACC G^GT[ACGT]ACC
BstPI 124 GGTTACC G^GT[ACGT]ACC
BstUI 3656 CGCG CG^CG
DraII 3991 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 4008 CCGTGG C^C[GA][CT]GG
EcoO109I 3991 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 124 GGTTACC G^GT[ACGT]ACC
EcoRI 2986 GAATTC G^AATTC
EcoT22I 1077 ATGCAT ATGCA^T
FauI 1373 GCGGG CCCGC(4/6)
FbaI 1054 TGATCA T^GATCA
HaeII 3377 AGCGCC [GA]GCGC^[CT]
KpnI 63 GGTACC GGTAC^C
MaeII 1862 ACGT A^CGT
MvnI 3656 CGCG CG^CG
NdeI 2387 CATATG CA^TATG
NsiI 1077 ATGCAT ATGCA^T
NspV 4214 TTCGAA TT^CGAA
PflFI 141 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3991 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1861 AACGTT AA^CGTT
PvuII 3319 CAGCTG CAG^CTG
RcaI 4425 TCATGA T^CATGA
SfcI 2713 CTGTAG C^T[GA][CT]AG
SfuI 4214 TTCGAA TT^CGAA
SmaI 3288 CCCGGG CCC^GGG
SspBI 3702 TGTACA T^GTACA
TaqI 4215 TCGA T^CGA
Tth111I 141 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 4215 TCGA T^CGA
XmaCI 3288 CCCGGG C^CCGGG
XmaI 3288 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 41 CCTGAGG CC^T[ACGT]AGG
3204 CCTCAGG
Asp700I 2316 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2964 GAAAAATTTC
BbvCI 3330 GCTGAGG CCTCAGC(-5/-2)
3616 GCTGAGG
BcnI 3288 CCCGG CC^[GC]GG
3289 CCGGG
BsgI 1410 CTGCAC GTGCAG(16/14)
888 GTGCAG
BspMI 1755 ACCTGC ACCTGC(4/8)
4076 GCAGGT
Bst1107I 880 GTATAC GTA^TAC
1478 GTATAC
BstZ17I 880 GTATAC GTA^TAC
1478 GTATAC
Bsu36I 41 CCTGAGG CC^T[ACGT]AGG
3204 CCTCAGG
Eco81I 41 CCTGAGG CC^T[ACGT]AGG
3204 CCTCAGG
HapII 67 CCGG C^CGG
3289 CCGG
HgaI 815 GACGC GACGC(5/10)
1448 GACGC
HincII 3484 GTTGAC GT[CT]^[GA]AC
3629 GTTGAC
HindII 3484 GTTGAC GT[CT]^[GA]AC
3629 GTTGAC
HpaII 67 CCGG C^CGG
3289 CCGG
MfeI 1085 CAATTG C^AATTG
1921 CAATTG
MspI 67 CCGG C^CGG
3289 CCGG
MunI 1085 CAATTG C^AATTG
1921 CAATTG
NciI 3288 CCCGG CC^[GC]GG
3289 CCGGG
PshAI 3195 GACCTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3310 GACATAAGTC
XbaI 1555 TCTAGA T^CTAGA
2661 TCTAGA
XmnI 2316 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2964 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaII BalI BanI BbvI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SmlI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII