##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI Asp700I
AspEI AspI AviII BbeI BbrPI
BfrI BglII BsaBI BseAI BsiWI
BspDI BspEI BssSI BstEII BstPI
ClaI CpoI Eam1105I Eco47III EcoNI
EcoO65I FseI FspI HindIII HpaI
KasI MamI MluI MroI NarI
NcoI NdeI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SpeI Sse8387I SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 3373 GACGTC GACGT^C
Acc65I 735 GGTACC G^GTACC
AccI 3788 GTATAC GT^[AC][GT]AC
AclI 636 AACGTT AA^CGTT
AocI 1505 CCTAAGG CC^T[ACGT]AGG
Asp718I 735 GGTACC G^GTACC
AspHI 3448 GTGCTC G[AT]GC[AT]^C
AvrII 2788 CCTAGG C^CTAGG
BalI 3908 TGGCCA TGG^CCA
BamHI 817 GGATCC G^GATCC
BanI 735 GGTACC G^G[CT][GA]CC
BcgI 4003 GCAGGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2220 GGATAC GTATCC(6/5)
BlnI 2788 CCTAGG C^CTAGG
BlpI 2933 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2933 GCTGAGC GC^T[ACGT]AGC
BsaAI 2495 TACGTA [CT]AC^GT[GA]
BsaI 437 GGTCTC GGTCTC(1/5)
BsgI 4060 CTGCAC GTGCAG(16/14)
BsiHKAI 3448 GTGCTC G[AT]GC[AT]^C
BsmBI 3928 CGTCTC CGTCTC(1/5)
BsmI 2389 GAATGC GAATGC(1/-1)
BspHI 3888 TCATGA T^CATGA
BsrDI 2000 CATTGC GCAATG(2/0)
BssHII 213 GCGCGC G^CGCGC
Bst1107I 3788 GTATAC GTA^TAC
BstAPI 1889 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3788 GTATAC GTA^TAC
Bsu36I 1505 CCTAAGG CC^T[ACGT]AGG
CelII 2933 GCTGAGC GC^T[ACGT]AGC
DraIII 345 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 202 CCGCGG C^C[GA][CT]GG
Eco81I 1505 CCTAAGG CC^T[ACGT]AGG
EcoRI 3396 GAATTC G^AATTC
EcoRV 1413 GATATC GAT^ATC
EcoT22I 1472 ATGCAT ATGCA^T
HincII 1935 GTTGAC GT[CT]^[GA]AC
HindII 1935 GTTGAC GT[CT]^[GA]AC
KpnI 735 GGTACC GGTAC^C
KspI 202 CCGCGG CCGC^GG
MluNI 3908 TGGCCA TGG^CCA
MscI 3908 TGGCCA TGG^CCA
NaeI 2645 GCCGGC GCC^GGC
NgoMIV 2645 GCCGGC G^CCGGC
NsiI 1472 ATGCAT ATGCA^T
PshAI 34 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 636 AACGTT AA^CGTT
RcaI 3888 TCATGA T^CATGA
SacII 202 CCGCGG CCGC^GG
SnaBI 2495 TACGTA TAC^GTA
SphI 1894 GCATGC GCATG^C
StuI 3836 AGGCCT AGG^CCT
XbaI 2385 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 1332 GACGCC G[GA]^CG[CT]C
3373 GACGTC
AflIII 3275 ACATGT A^C[GA][CT]GT
3562 ACATGT
AvaI 92 CCCGGG C^[CT]CG[GA]G
265 CCCGGG
BbsI 938 GAAGAC GAAGAC(2/6)
1602 GTCTTC
BbvCI 3863 GCTGAGG CCTCAGC(-5/-2)
3997 GCTGAGG
BclI 625 TGATCA T^GATCA
3216 TGATCA
BpuAI 938 GAAGAC GAAGAC(2/6)
1602 GTCTTC
BsaHI 1332 GACGCC G[GA]^CG[CT]C
3373 GACGTC
BsaWI 491 TCCGGT [AT]^CCGG[AT]
3053 ACCGGA
BseRI 13 GAGGAG GAGGAG(10/8)
898 GAGGAG
BsoBI 92 CCCGGG C^[CT]CG[GA]G
265 CCCGGG
Bsp1407I 762 TGTACA T^GTACA
1768 TGTACA
BspLU11I 3275 ACATGT A^CATGT
3562 ACATGT
BspMI 1939 ACCTGC ACCTGC(4/8)
2718 ACCTGC
BsrBI 183 CCGCTC CCGCTC(-3/-3)
240 GAGCGG
BsrFI 391 ACCGGC [GA]^CCGG[CT]
2645 GCCGGC
BsrGI 762 TGTACA T^GTACA
1768 TGTACA
BstBI 1605 TTCGAA TT^CGAA
3895 TTCGAA
Cfr10I 391 ACCGGC [GA]^CCGG[CT]
2645 GCCGGC
DrdI 549 GACTGTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1742 GACTTTCTTGTC
EagI 229 CGGCCG C^GGCCG
393 CGGCCG
EclXI 229 CGGCCG C^GGCCG
393 CGGCCG
Eco52I 229 CGGCCG C^GGCCG
393 CGGCCG
FbaI 625 TGATCA T^GATCA
3216 TGATCA
HaeII 353 GGCGCT [GA]GCGC^[CT]
609 GGCGCT
Hin1I 1332 GACGCC G[GA]^CG[CT]C
3373 GACGTC
MfeI 2681 CAATTG C^AATTG
2741 CAATTG
MunI 2681 CAATTG C^AATTG
2741 CAATTG
NheI 132 GCTAGC G^CTAGC
1284 GCTAGC
NspV 1605 TTCGAA TT^CGAA
3895 TTCGAA
PflMI 344 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1088 CCAGTGTGTGG
PpuMI 2850 AGGACCC [GA]G^G[AT]CC[CT]
3761 GGGACCC
PstI 1851 CTGCAG CTGCA^G
1887 CTGCAG
SfuI 1605 TTCGAA TT^CGAA
3895 TTCGAA
SmaI 92 CCCGGG CCC^GGG
265 CCCGGG
SspBI 762 TGTACA T^GTACA
1768 TGTACA
SspI 3455 AATATT AAT^ATT
3755 AATATT
Van91I 344 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1088 CCAGTGTGTGG
XcmI 340 CCATCCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3117 CCACTGCCACCTTGG
XmaCI 92 CCCGGG C^CCGGG
265 CCCGGG
XmaI 92 CCCGGG C^CCGGG
265 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AseI AsnI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII