##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AccIII AclI AflII AscI AseI
AsnI AspI AviII BbrPI BbvCI
BciVI BclI BfrI BglII BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BsrGI
BstBI BstEII BstPI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MluI MroI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 521 GACGTC GACGT^C
AccII 3256 CGCG CG^CG
AgeI 2212 ACCGGT A^CCGGT
AhdI 110 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 499 AGCGCT AGC^GCT
Asp700I 497 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 110 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2708 CCTAGG C^CTAGG
BbeI 2932 GGCGCC GGCGC^C
BcnI 596 CCGGG CC^[GC]GG
BglI 1813 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2708 CCTAGG C^CTAGG
BsaWI 2212 ACCGGT [AT]^CCGG[AT]
BspDI 906 ATCGAT AT^CGAT
BsrBI 1641 CCGCTC CCGCTC(-3/-3)
BsrDI 3278 CATTGC GCAATG(2/0)
BssHII 3255 GCGCGC G^CGCGC
Bst1107I 2689 GTATAC GTA^TAC
BstAPI 2342 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3256 CGCG CG^CG
BstZ17I 2689 GTATAC GTA^TAC
ClaI 906 ATCGAT AT^CGAT
Eam1105I 110 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 499 AGCGCT AGC^GCT
KasI 2932 GGCGCC G^GCGCC
MfeI 2346 CAATTG C^AATTG
MunI 2346 CAATTG C^AATTG
MvnI 3256 CGCG CG^CG
NarI 2932 GGCGCC GG^CGCC
NciI 596 CCGGG CC^[GC]GG
NcoI 1420 CCATGG C^CATGG
PinAI 2212 ACCGGT A^CCGGT
SapI 1643 GCTCTTC GCTCTTC(1/4)
SexAI 3440 ACCTGGT A^CC[AT]GGT
SfoI 2932 GGCGCC GGC^GCC
XmnI 497 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 296 GGTACC G^GTACC
305 GGTACC
AccI 2689 GTATAC GT^[AC][GT]AC
2970 GTAGAC
AflIII 3295 ACATGT A^C[GA][CT]GT
3768 ACATGT
Alw44I 600 GTGCAC G^TGCAC
2145 GTGCAC
ApaLI 600 GTGCAC G^TGCAC
2145 GTGCAC
Asp718I 296 GGTACC G^GTACC
305 GGTACC
BcgI 908 CGATGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2122 GCAGCGCCATCG
BlpI 208 GCTAAGC GC^T[ACGT]AGC
3502 GCTCAGC
Bpu1102I 208 GCTAAGC GC^T[ACGT]AGC
3502 GCTCAGC
BspHI 454 TCATGA T^CATGA
658 TCATGA
BspLU11I 3295 ACATGT A^CATGT
3768 ACATGT
BsrFI 1116 GCCGGT [GA]^CCGG[CT]
2212 ACCGGT
BssSI 1789 CTCGTG CACGAG(-5/-1)
3537 CTCGTG
BstXI 1450 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3671 CCACCTCGCTGG
CelII 208 GCTAAGC GC^T[ACGT]AGC
3502 GCTCAGC
Cfr10I 1116 GCCGGT [GA]^CCGG[CT]
2212 ACCGGT
EarI 1626 CTCTTC CTCTTC(1/4)
1644 CTCTTC
HgaI 1552 GCGTC GACGC(5/10)
2168 GCGTC
KpnI 296 GGTACC GGTAC^C
305 GGTACC
Ksp632I 1626 CTCTTC CTCTTC(1/4)
1644 CTCTTC
PflMI 1041 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1940 CCATCTGGTGG
RcaI 454 TCATGA T^CATGA
658 TCATGA
SmlI 797 CTCAAG C^T[CT][GA]AG
1826 CTCAAG
Van91I 1041 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1940 CCATCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII