##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbeI BbrPI BclI BglII
BsaAI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII BstBI BstEII
BstPI ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI KpnI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 2756 CTTAAG C^TTAAG
AflIII 2672 ACGTGT A^C[GA][CT]GT
AhdI 1022 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2656 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1022 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3803 CCTAGG C^CTAGG
BbsI 1185 GTCTTC GAAGAC(2/6)
BbvCI 1939 GCTGAGG CCTCAGC(-5/-2)
BcgI 3501 GCACCAGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 40 GTATCC GTATCC(6/5)
BfrI 2756 CTTAAG C^TTAAG
BlnI 3803 CCTAGG C^CTAGG
BpuAI 1185 GTCTTC GAAGAC(2/6)
BsaBI 2941 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1087 GAATGC GAATGC(1/-1)
BspHI 3690 TCATGA T^CATGA
BsrDI 1111 CATTGC GCAATG(2/0)
BsrFI 1653 ACCGGC [GA]^CCGG[CT]
Bst1107I 527 GTATAC GTA^TAC
BstXI 3975 CCACCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 527 GTATAC GTA^TAC
Cfr10I 1653 ACCGGC [GA]^CCGG[CT]
DrdI 173 GACCGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1022 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 134 GAATTC G^AATTC
HindIII 4212 AAGCTT A^AGCTT
KspI 3474 CCGCGG CCGC^GG
MamI 2941 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 1445 CTCGAG C^TCGAG
RcaI 3690 TCATGA T^CATGA
SacII 3474 CCGCGG CCGC^GG
SalI 1175 GTCGAC G^TCGAC
SspI 3832 AATATT AAT^ATT
XhoI 1445 CTCGAG C^TCGAG
XmnI 2656 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AatII 1022 GACGTC GACGT^C
4048 GACGTC
AccI 527 GTATAC GT^[AC][GT]AC
1175 GTCGAC
BsmBI 3312 CGTCTC CGTCTC(1/5)
2669 GAGACG
BstAPI 2063 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2578 GCAAGAGTTGC
HincII 1175 GTCGAC GT[CT]^[GA]AC
1505 GTCAAC
HindII 1175 GTCGAC GT[CT]^[GA]AC
1505 GTCAAC
MslI 348 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
848 CATAGGGATG
PshAI 1146 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1178 GACTCCTGTC
ScaI 1158 AGTACT AGT^ACT
2313 AGTACT
SphI 2596 GCATGC GCATG^C
3285 GCATGC
StuI 3114 AGGCCT AGG^CCT
4151 AGGCCT
XbaI 748 TCTAGA T^CTAGA
3870 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI