##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AspEI AviII AvrII
BamHI BbrPI BfrI BglII BlnI
BsaBI BsiEI BsiWI BstEII BstPI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT22I FseI
FspI MamI MluI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SalI SbfI SexAI SnaBI
SpeI Sse8387I StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 217 GGTACC G^GTACC
AccIII 3812 TCCGGA T^CCGGA
AcyI 3881 GGCGCC G[GA]^CG[CT]C
AocI 2371 CCTGAGG CC^T[ACGT]AGG
ApaI 40 GGGCCC GGGCC^C
AseI 4172 ATTAAT AT^TAAT
AsnI 4172 ATTAAT AT^TAAT
Asp700I 4566 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 217 GGTACC G^GTACC
BbeI 3881 GGCGCC GGCGC^C
BbvCI 615 CCTCAGC CCTCAGC(-5/-2)
BciVI 2490 GGATAC GTATCC(6/5)
BlpI 2346 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2346 GCTCAGC GC^T[ACGT]AGC
BsaAI 3388 TACGTG [CT]AC^GT[GA]
BsaHI 3881 GGCGCC G[GA]^CG[CT]C
BseAI 3812 TCCGGA T^CCGGA
BsmBI 1365 CGTCTC CGTCTC(1/5)
BspDI 869 ATCGAT AT^CGAT
BspEI 3812 TCCGGA T^CCGGA
BspHI 4665 TCATGA T^CATGA
BspMI 4992 ACCTGC ACCTGC(4/8)
BssHII 1 GCGCGC G^CGCGC
Bst1107I 4192 GTATAC GTA^TAC
BstAPI 2874 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1850 TTCGAA TT^CGAA
BstXI 1985 CCAGAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4192 GTATAC GTA^TAC
Bsu36I 2371 CCTGAGG CC^T[ACGT]AGG
CelII 2346 GCTCAGC GC^T[ACGT]AGC
ClaI 869 ATCGAT AT^CGAT
Eco81I 2371 CCTGAGG CC^T[ACGT]AGG
EcoNI 5242 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 403 GATATC GAT^ATC
Hin1I 3881 GGCGCC G[GA]^CG[CT]C
HindIII 1928 AAGCTT A^AGCTT
HpaI 5237 GTTAAC GTT^AAC
KasI 3881 GGCGCC G^GCGCC
KpnI 217 GGTACC GGTAC^C
KspI 59 CCGCGG CCGC^GG
MfeI 1139 CAATTG C^AATTG
MroI 3812 TCCGGA T^CCGGA
MunI 1139 CAATTG C^AATTG
NarI 3881 GGCGCC GG^CGCC
NdeI 4831 CATATG CA^TATG
NspV 1850 TTCGAA TT^CGAA
PflMI 2478 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 4172 ATTAAT AT^TAAT
PstI 151 CTGCAG CTGCA^G
RcaI 4665 TCATGA T^CATGA
SacII 59 CCGCGG CCGC^GG
SfcI 151 CTGCAG C^T[GA][CT]AG
SfiI 3417 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3881 GGCGCC GGC^GCC
SfuI 1850 TTCGAA TT^CGAA
SgrAI 171 CGCCGGCG C[GA]^CCGG[CT]G
Van91I 2478 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 4566 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 2970 ACATGT A^C[GA][CT]GT
3188 ACATGT
Alw44I 962 GTGCAC G^TGCAC
1997 GTGCAC
ApaLI 962 GTGCAC G^TGCAC
1997 GTGCAC
AspI 2038 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3631 GACACGGTC
BbsI 1702 GAAGAC GAAGAC(2/6)
3199 GAAGAC
BcgI 2669 GCATTGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3275 GCAAGTTGTTCG
BclI 734 TGATCA T^GATCA
2898 TGATCA
BpuAI 1702 GAAGAC GAAGAC(2/6)
3199 GAAGAC
BsaWI 677 TCCGGT [AT]^CCGG[AT]
3812 TCCGGA
BsmI 708 GAATGC GAATGC(1/-1)
3123 GAATGC
Bsp1407I 2864 TGTACA T^GTACA
2965 TGTACA
BspLU11I 2970 ACATGT A^CATGT
3188 ACATGT
BsrDI 441 CATTGC GCAATG(2/0)
1812 CATTGC
BsrGI 2864 TGTACA T^GTACA
2965 TGTACA
BstYI 1256 GGATCT [GA]^GATC[CT]
1571 GGATCT
DrdI 286 GACTCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2008 GACCAAGGTGTC
FbaI 734 TGATCA T^GATCA
2898 TGATCA
HgaI 2481 GACGC GACGC(5/10)
1439 GCGTC
MflI 1256 GGATCT [GA]^GATC[CT]
1571 GGATCT
NcoI 1721 CCATGG C^CATGG
2832 CCATGG
NheI 2028 GCTAGC G^CTAGC
4984 GCTAGC
PflFI 2038 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3631 GACACGGTC
PpuMI 590 AGGTCCC [GA]G^G[AT]CC[CT]
2837 GGGACCT
PvuII 2050 CAGCTG CAG^CTG
2595 CAGCTG
SacI 1392 GAGCTC GAGCT^C
1642 GAGCTC
SapI 2701 GCTCTTC GCTCTTC(1/4)
3357 GCTCTTC
SmlI 355 CTTGAG C^T[CT][GA]AG
700 CTTGAG
SspBI 2864 TGTACA T^GTACA
2965 TGTACA
Tth111I 2038 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3631 GACACGGTC
XhoII 1256 GGATCT [GA]^GATC[CT]
1571 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SmaI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI