##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI Alw44I ApaI ApaLI AscI
AspI AviII AvrII BbeI BbrPI
BbsI BcgI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrFI BssHII Bst1107I
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FseI FspI
KasI KspI MamI MluI MroI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1086 GGTACC G^GTACC
AccI 1360 GTCGAC GT^[AC][GT]AC
AcyI 2807 GGCGTC G[GA]^CG[CT]C
AhdI 1449 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4268 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2403 AGCGCT AGC^GCT
Asp718I 1086 GGTACC G^GTACC
AspEI 1449 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1432 CCCGAG C^[CT]CG[GA]G
BamHI 835 GGATCC G^GATCC
BciVI 549 GTATCC GTATCC(6/5)
BcnI 3649 CCCGG CC^[GC]GG
BglII 131 AGATCT A^GATCT
BsaHI 2807 GGCGTC G[GA]^CG[CT]C
BsgI 2353 GTGCAG GTGCAG(16/14)
BsoBI 1432 CCCGAG C^[CT]CG[GA]G
BspHI 4082 TCATGA T^CATGA
BsrBI 1952 CCGCTC CCGCTC(-3/-3)
BssSI 421 CTCGTG CACGAG(-5/-1)
BstAPI 2027 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3599 GGTGACC G^GT[ACGT]ACC
BstPI 3599 GGTGACC G^GT[ACGT]ACC
Bsu36I 4268 CCTGAGG CC^T[ACGT]AGG
DsaI 4214 CCGTGG C^C[GA][CT]GG
Eam1105I 1449 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2403 AGCGCT AGC^GCT
Eco81I 4268 CCTGAGG CC^T[ACGT]AGG
EcoNI 3046 CCTAAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3599 GGTGACC G^GT[ACGT]ACC
HaeII 2403 AGCGCT [GA]GCGC^[CT]
HapII 3650 CCGG C^CGG
HgaI 2808 GCGTC GACGC(5/10)
Hin1I 2807 GGCGTC G[GA]^CG[CT]C
HpaI 3670 GTTAAC GTT^AAC
HpaII 3650 CCGG C^CGG
KpnI 1086 GGTACC GGTAC^C
MspA1I 535 CAGCTG C[AC]G^C[GT]G
MspI 3650 CCGG C^CGG
NciI 3649 CCCGG CC^[GC]GG
NdeI 3369 CATATG CA^TATG
PvuII 535 CAGCTG CAG^CTG
RcaI 4082 TCATGA T^CATGA
SalI 1360 GTCGAC G^TCGAC
SmlI 67 CTCAAG C^T[CT][GA]AG
XbaI 1668 TCTAGA T^CTAGA
XcmI 3571 CCAGGCTGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 3942 ACATGT A^C[GA][CT]GT
4017 ACATGT
AlwNI 1260 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2488 CAGGTTCTG
AseI 39 ATTAAT AT^TAAT
3873 ATTAAT
AsnI 39 ATTAAT AT^TAAT
3873 ATTAAT
BanI 1086 GGTACC G^G[CT][GA]CC
4210 GGCACC
BanII 2648 GGGCTC G[GA]GC[CT]^C
3592 GGGCTC
BbvCI 3968 GCTGAGG CCTCAGC(-5/-2)
4385 GCTGAGG
BsaI 335 GAGACC GGTCTC(1/5)
4285 GAGACC
BsmI 3840 GAATGC GAATGC(1/-1)
1198 GCATTC
BspLU11I 3942 ACATGT A^CATGT
4017 ACATGT
BsrDI 2032 CATTGC GCAATG(2/0)
4103 CATTGC
BstBI 997 TTCGAA TT^CGAA
2200 TTCGAA
BstXI 2411 CCAATATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4235 CCAGCACTTTGG
CfoI 2303 GCGC GCG^C
2404 GCGC
DraII 479 AGGACCT [GA]G^G[ACGT]CC[CT]
3659 GGGACCC
DraIII 1496 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1978 CACAAAGTG
EcoO109I 479 AGGACCT [GA]G^G[ACGT]CC[CT]
3659 GGGACCC
HhaI 2303 GCGC GCG^C
2404 GCGC
HinP1I 2303 GCGC G^CGC
2404 GCGC
MaeII 2466 ACGT A^CGT
3122 ACGT
MfeI 3679 CAATTG C^AATTG
4138 CAATTG
MunI 3679 CAATTG C^AATTG
4138 CAATTG
NspV 997 TTCGAA TT^CGAA
2200 TTCGAA
PpuMI 479 AGGACCT [GA]G^G[AT]CC[CT]
3659 GGGACCC
PshBI 39 ATTAAT AT^TAAT
3873 ATTAAT
PstI 2712 CTGCAG CTGCA^G
3755 CTGCAG
SapI 2514 GAAGAGC GCTCTTC(1/4)
1038 GCTCTTC
SfuI 997 TTCGAA TT^CGAA
2200 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BalI
BbvI BclI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI