##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccII AccIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AvrII BamHI BbrPI BcgI
BciVI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
Cfr10I ClaI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
HindIII KspI MamI MluI MroI
MvnI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PvuI SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 471 GGTACC G^GTACC
AclI 872 AACGTT AA^CGTT
AcyI 3051 GGCGCC G[GA]^CG[CT]C
AflIII 362 ACGTGT A^C[GA][CT]GT
AlwNI 2617 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2239 CCTGAGG CC^T[ACGT]AGG
ApaI 2647 GGGCCC GGGCC^C
Asp700I 3451 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 471 GGTACC G^GTACC
AspI 2105 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2445 TGCGCA TGC^GCA
BanII 2647 GGGCCC G[GA]GC[CT]^C
BbeI 3051 GGCGCC GGCGC^C
BbsI 155 GAAGAC GAAGAC(2/6)
BbvCI 1787 GCTGAGG CCTCAGC(-5/-2)
BclI 2857 TGATCA T^GATCA
BlpI 27 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 27 GCTGAGC GC^T[ACGT]AGC
BpuAI 155 GAAGAC GAAGAC(2/6)
BsaHI 3051 GGCGCC G[GA]^CG[CT]C
BspHI 2038 TCATGA T^CATGA
BssSI 2968 CTCGTG CACGAG(-5/-1)
Bsu36I 2239 CCTGAGG CC^T[ACGT]AGG
CelII 27 GCTGAGC GC^T[ACGT]AGC
CpoI 651 CGGACCG CG^G[AT]CCG
DrdI 1950 GACTTTTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2239 CCTGAGG CC^T[ACGT]AGG
EcoNI 1874 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1115 GAATTC G^AATTC
EcoRV 1196 GATATC GAT^ATC
FauI 978 CCCGC CCCGC(4/6)
FbaI 2857 TGATCA T^GATCA
FspI 2445 TGCGCA TGC^GCA
Hin1I 3051 GGCGCC G[GA]^CG[CT]C
HpaI 526 GTTAAC GTT^AAC
KasI 3051 GGCGCC G^GCGCC
KpnI 471 GGTACC GGTAC^C
MfeI 2390 CAATTG C^AATTG
MunI 2390 CAATTG C^AATTG
NarI 3051 GGCGCC GG^CGCC
NdeI 3296 CATATG CA^TATG
PflFI 2105 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3891 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 872 AACGTT AA^CGTT
RcaI 2038 TCATGA T^CATGA
RsrII 651 CGGACCG CG^G[AT]CCG
SfoI 3051 GGCGCC GGC^GCC
SmaI 784 CCCGGG CCC^GGG
StuI 1919 AGGCCT AGG^CCT
Tth111I 2105 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1974 CCAAGAGGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 784 CCCGGG C^CCGGG
XmaI 784 CCCGGG C^CCGGG
XmnI 3451 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 147 CTTAAG C^TTAAG
3043 CTTAAG
AseI 2845 ATTAAT AT^TAAT
3205 ATTAAT
AsnI 2845 ATTAAT AT^TAAT
3205 ATTAAT
AvaI 784 CCCGGG C^[CT]CG[GA]G
1467 CCCGAG
BanI 471 GGTACC G^G[CT][GA]CC
3051 GGCGCC
BfrI 147 CTTAAG C^TTAAG
3043 CTTAAG
BsmAI 1326 GAGAC GTCTC(1/5)
4069 GAGAC
BsmI 3596 GAATGC GAATGC(1/-1)
3247 GCATTC
BsoBI 784 CCCGGG C^[CT]CG[GA]G
1467 CCCGAG
BspMI 603 ACCTGC ACCTGC(4/8)
1930 GCAGGT
BsrDI 673 CATTGC GCAATG(2/0)
1272 CATTGC
BstEII 1348 GGTGACC G^GT[ACGT]ACC
1364 GGTGACC
BstPI 1348 GGTGACC G^GT[ACGT]ACC
1364 GGTGACC
BstXI 451 CCACAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1848 CCAAGGATGTGG
BstYI 1862 GGATCT [GA]^GATC[CT]
2796 AGATCC
DraIII 495 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2174 CACTCAGTG
EcoO65I 1348 GGTGACC G^GT[ACGT]ACC
1364 GGTGACC
HgaI 1281 GACGC GACGC(5/10)
2379 GCGTC
HincII 526 GTTAAC GT[CT]^[GA]AC
1292 GTTGAC
HindII 526 GTTAAC GT[CT]^[GA]AC
1292 GTTGAC
MflI 1862 GGATCT [GA]^GATC[CT]
2796 AGATCC
PleI 793 GAGTC GAGTC(4/5)
2637 GAGTC
PpuMI 3637 AGGACCC [GA]G^G[AT]CC[CT]
3853 GGGTCCC
PshBI 2845 ATTAAT AT^TAAT
3205 ATTAAT
PstI 2363 CTGCAG CTGCA^G
3626 CTGCAG
PvuII 777 CAGCTG CAG^CTG
878 CAGCTG
SspI 406 AATATT AAT^ATT
1382 AATATT
XhoII 1862 GGATCT [GA]^GATC[CT]
2796 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I