##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbrPI BbvCI BcgI BglII
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BspDI BspEI BspHI BsrDI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI FspI HgaI
HindIII KpnI KspI MamI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfuI SgrAI
SnaBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 1539 CGCG CG^CG
AcyI 1 GGCGCC G[GA]^CG[CT]C
AflII 3221 CTTAAG C^TTAAG
AflIII 2289 ACATGT A^C[GA][CT]GT
AlwNI 2362 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 3610 AGCGCT AGC^GCT
BbeI 1 GGCGCC GGCGC^C
BciVI 647 GGATAC GTATCC(6/5)
BclI 535 TGATCA T^GATCA
BfrI 3221 CTTAAG C^TTAAG
BglI 881 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3176 TACGTG [CT]AC^GT[GA]
BsaHI 1 GGCGCC G[GA]^CG[CT]C
Bsp1407I 133 TGTACA T^GTACA
BspLU11I 2289 ACATGT A^CATGT
BsrFI 3589 GCCGGT [GA]^CCGG[CT]
BsrGI 133 TGTACA T^GTACA
BssHII 1538 GCGCGC G^CGCGC
BstAPI 3388 GCACTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1539 CGCG CG^CG
BstXI 3083 CCAGATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3589 GCCGGT [GA]^CCGG[CT]
DraIII 570 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3905 CCATGG C^C[GA][CT]GG
Eco47III 3610 AGCGCT AGC^GCT
EcoRI 3249 GAATTC G^AATTC
FbaI 535 TGATCA T^GATCA
Hin1I 1 GGCGCC G[GA]^CG[CT]C
HincII 4425 GTTAAC GT[CT]^[GA]AC
HindII 4425 GTTAAC GT[CT]^[GA]AC
HpaI 4425 GTTAAC GTT^AAC
KasI 1 GGCGCC G^GCGCC
MfeI 4121 CAATTG C^AATTG
MunI 4121 CAATTG C^AATTG
MvnI 1539 CGCG CG^CG
NarI 1 GGCGCC GG^CGCC
NcoI 3905 CCATGG C^CATGG
PaeR7I 1110 CTCGAG C^TCGAG
PshAI 3106 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4249 GAGCTC GAGCT^C
SbfI 199 CCTGCAGG CCTGCA^GG
SfiI 880 GGCCTACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1 GGCGCC GGC^GCC
SmaI 2001 CCCGGG CCC^GGG
SpeI 1674 ACTAGT A^CTAGT
Sse8387I 199 CCTGCAGG CCTGCA^GG
SspBI 133 TGTACA T^GTACA
XbaI 3001 TCTAGA T^CTAGA
XhoI 1110 CTCGAG C^TCGAG
XmaCI 2001 CCCGGG C^CCGGG
XmaI 2001 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Asp700I 1190 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3560 GAAACAGTTC
AvaI 1110 CTCGAG C^[CT]CG[GA]G
2001 CCCGGG
AvrII 3584 CCTAGG C^CTAGG
3620 CCTAGG
BbsI 3740 GAAGAC GAAGAC(2/6)
452 GTCTTC
BlnI 3584 CCTAGG C^CTAGG
3620 CCTAGG
BpmI 2533 CTGGAG CTGGAG(16/14)
2808 CTGGAG
BpuAI 3740 GAAGAC GAAGAC(2/6)
452 GTCTTC
BsmBI 994 GAGACG CGTCTC(1/5)
1120 GAGACG
BsoBI 1110 CTCGAG C^[CT]CG[GA]G
2001 CCCGGG
BspMI 3728 ACCTGC ACCTGC(4/8)
202 GCAGGT
HaeII 1 GGCGCC [GA]GCGC^[CT]
3610 AGCGCT
PpuMI 1314 AGGACCT [GA]G^G[AT]CC[CT]
3290 AGGACCT
SexAI 810 ACCTGGT A^CC[AT]GGT
2231 ACCAGGT
SphI 623 GCATGC GCATG^C
2295 GCATGC
XcmI 1577 CCACCCTTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3785 CCACATCACCTCTGG
XmnI 1190 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3560 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AocI ApaLI ApoI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII