##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccII AccIII AclI AlwNI
Aor51HI ApaI AscI Asp700I AspI
AvaI AviII BamHI BbeI BbrPI
BbvCI BcgI BglI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HindIII KasI
KspI MluI MroI MvnI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 786 GGTACC G^GTACC
AcyI 2827 GACGCC G[GA]^CG[CT]C
AflIII 111 ACATGT A^C[GA][CT]GT
AgeI 1705 ACCGGT A^CCGGT
Alw44I 3166 GTGCAC G^TGCAC
ApaLI 3166 GTGCAC G^TGCAC
AseI 2123 ATTAAT AT^TAAT
AsnI 2123 ATTAAT AT^TAAT
Asp718I 786 GGTACC G^GTACC
AvrII 3370 CCTAGG C^CTAGG
BalI 3846 TGGCCA TGG^CCA
BanI 786 GGTACC G^G[CT][GA]CC
BcnI 12 CCGGG CC^[GC]GG
BglII 3109 AGATCT A^GATCT
BlnI 3370 CCTAGG C^CTAGG
BlpI 2042 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2042 GCTAAGC GC^T[ACGT]AGC
BsaAI 1512 CACGTA [CT]AC^GT[GA]
BsaBI 3563 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2827 GACGCC G[GA]^CG[CT]C
BsaWI 1705 ACCGGT [AT]^CCGG[AT]
BspLU11I 111 ACATGT A^CATGT
CelII 2042 GCTAAGC GC^T[ACGT]AGC
CfoI 671 GCGC GCG^C
EcoNI 2995 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 3370 CCTAGG C^C[AT][AT]GG
HgaI 2827 GACGC GACGC(5/10)
HhaI 671 GCGC GCG^C
Hin1I 2827 GACGCC G[GA]^CG[CT]C
HinP1I 671 GCGC G^CGC
KpnI 786 GGTACC GGTAC^C
MamI 3563 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3846 TGGCCA TGG^CCA
MscI 3846 TGGCCA TGG^CCA
MslI 447 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 273 CAGCTG C[AC]G^C[GT]G
NaeI 3123 GCCGGC GCC^GGC
NciI 12 CCGGG CC^[GC]GG
NgoMIV 3123 GCCGGC G^CCGGC
PflMI 1049 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1705 ACCGGT A^CCGGT
PshAI 867 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2123 ATTAAT AT^TAAT
PvuII 273 CAGCTG CAG^CTG
SacI 1174 GAGCTC GAGCT^C
SapI 1796 GCTCTTC GCTCTTC(1/4)
SpeI 1385 ACTAGT A^CTAGT
SphI 2507 GCATGC GCATG^C
StuI 3367 AGGCCT AGG^CCT
StyI 3370 CCTAGG C^C[AT][AT]GG
SwaI 3538 ATTTAAAT ATTT^AAAT
Tsp45I 803 GTCAC ^GT[GC]AC
Van91I 1049 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2458 TCTAGA T^CTAGA
XcmI 2240 CCAGGCTATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 1484 CCTGAGG CC^T[ACGT]AGG
2999 CCTAAGG
BanII 1174 GAGCTC G[GA]GC[CT]^C
1775 GAGCCC
BciVI 685 GTATCC GTATCC(6/5)
736 GTATCC
BclI 295 TGATCA T^GATCA
2915 TGATCA
BseRI 1760 GAGGAG GAGGAG(10/8)
1877 GAGGAG
BsgI 1158 CTGCAC GTGCAG(16/14)
2822 GTGCAG
BsmI 1852 GAATGC GAATGC(1/-1)
47 GCATTC
BspHI 997 TCATGA T^CATGA
2599 TCATGA
BspMI 2026 ACCTGC ACCTGC(4/8)
358 GCAGGT
BsrFI 1705 ACCGGT [GA]^CCGG[CT]
3123 GCCGGC
Bst1107I 706 GTATAC GTA^TAC
1515 GTATAC
BstYI 441 AGATCC [GA]^GATC[CT]
3109 AGATCT
BstZ17I 706 GTATAC GTA^TAC
1515 GTATAC
Bsu36I 1484 CCTGAGG CC^T[ACGT]AGG
2999 CCTAAGG
Cfr10I 1705 ACCGGT [GA]^CCGG[CT]
3123 GCCGGC
DraII 132 GGGACCC [GA]G^G[ACGT]CC[CT]
2023 AGGACCT
Eco81I 1484 CCTGAGG CC^T[ACGT]AGG
2999 CCTAAGG
EcoO109I 132 GGGACCC [GA]G^G[ACGT]CC[CT]
2023 AGGACCT
EcoRI 1184 GAATTC G^AATTC
2216 GAATTC
FbaI 295 TGATCA T^GATCA
2915 TGATCA
HincII 2721 GTTAAC GT[CT]^[GA]AC
2964 GTTAAC
HindII 2721 GTTAAC GT[CT]^[GA]AC
2964 GTTAAC
HpaI 2721 GTTAAC GTT^AAC
2964 GTTAAC
MfeI 569 CAATTG C^AATTG
3766 CAATTG
MflI 441 AGATCC [GA]^GATC[CT]
3109 AGATCT
MunI 569 CAATTG C^AATTG
3766 CAATTG
NdeI 538 CATATG CA^TATG
2817 CATATG
PpuMI 132 GGGACCC [GA]G^G[AT]CC[CT]
2023 AGGACCT
RcaI 997 TCATGA T^CATGA
2599 TCATGA
SspI 167 AATATT AAT^ATT
3487 AATATT
XhoII 441 AGATCC [GA]^GATC[CT]
3109 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AhdI AluI AlwI ApoI AspEI
AspHI AvaII BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EaeI Eam1105I EarI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I