##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BglI BlnI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BsiWI
BsoBI BspDI BspEI BspLU11I BssHII
BstAPI BstBI BstEII BstPI CelII
ClaI CpoI DsaI Eam1105I Eco47III
EcoO65I EcoRV FseI FspI HaeII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 4128 GTATAC GT^[AC][GT]AC
AcyI 84 GACGCC G[GA]^CG[CT]C
AlwNI 545 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 144 GGGCCC GGGCC^C
AseI 2021 ATTAAT AT^TAAT
AsnI 2021 ATTAAT AT^TAAT
BamHI 2207 GGATCC G^GATCC
BclI 1618 TGATCA T^GATCA
BglII 2323 AGATCT A^GATCT
BsaBI 938 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 84 GACGCC G[GA]^CG[CT]C
BsiEI 133 CGGCCG CG[GA][CT]^CG
BsmBI 2660 CGTCTC CGTCTC(1/5)
BsmFI 2841 GGGAC GGGAC(10/14)
Bsp1407I 4006 TGTACA T^GTACA
BspHI 1201 TCATGA T^CATGA
BspMI 3191 ACCTGC ACCTGC(4/8)
BsrGI 4006 TGTACA T^GTACA
Bst1107I 4128 GTATAC GTA^TAC
BstXI 3072 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4128 GTATAC GTA^TAC
CfoI 60 GCGC GCG^C
DraIII 1896 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 133 CGGCCG C^GGCCG
EclXI 133 CGGCCG C^GGCCG
Eco52I 133 CGGCCG C^GGCCG
EcoNI 322 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1618 TGATCA T^GATCA
HhaI 60 GCGC GCG^C
Hin1I 84 GACGCC G[GA]^CG[CT]C
HinP1I 60 GCGC G^CGC
MamI 938 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1059 CATATG CA^TATG
PpuMI 2841 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1799 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2021 ATTAAT AT^TAAT
RcaI 1201 TCATGA T^CATGA
SpeI 1775 ACTAGT A^CTAGT
SspBI 4006 TGTACA T^GTACA
SwaI 4030 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 3024 CTTAAG C^TTAAG
3525 CTTAAG
AocI 209 CCTCAGG CC^T[ACGT]AGG
376 CCTGAGG
BciVI 3707 GGATAC GTATCC(6/5)
3113 GTATCC
BfrI 3024 CTTAAG C^TTAAG
3525 CTTAAG
BsgI 2625 GTGCAG GTGCAG(16/14)
2902 GTGCAG
BsrBI 183 CCGCTC CCGCTC(-3/-3)
1153 CCGCTC
BssSI 702 CACGAG CACGAG(-5/-1)
3177 CTCGTG
Bsu36I 209 CCTCAGG CC^T[ACGT]AGG
376 CCTGAGG
DrdI 1191 GACTAACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2267 GACAAATACGTC
EaeI 133 CGGCCG [CT]^GGCC[GA]
150 TGGCCG
Eco81I 209 CCTCAGG CC^T[ACGT]AGG
376 CCTGAGG
EcoT22I 2339 ATGCAT ATGCA^T
3295 ATGCAT
FauI 1152 CCCGC CCCGC(4/6)
62 GCGGG
HgaI 57 GACGC GACGC(5/10)
84 GACGC
HpaI 512 GTTAAC GTT^AAC
3680 GTTAAC
NaeI 131 GCCGGC GCC^GGC
155 GCCGGC
NgoMIV 131 GCCGGC G^CCGGC
155 GCCGGC
NsiI 2339 ATGCAT ATGCA^T
3295 ATGCAT
PstI 2943 CTGCAG CTGCA^G
3221 CTGCAG
PvuII 1134 CAGCTG CAG^CTG
2070 CAGCTG
StuI 823 AGGCCT AGG^CCT
1376 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI