##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AclI AflII Alw44I Aor51HI ApaI
ApaLI AseI AsnI AviII AvrII
BcgI BfrI BglI BlnI BsiWI
BspDI BstBI ClaI CpoI DraIII
Eco47III EcoRV FseI FspI MluI
NotI NruI NspV PacI PmeI
PshBI Psp1406I PvuI RsrII SexAI
SfiI SfuI SgrAI SmaI SnaBI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 75 TCCGGA T^CCGGA
AgeI 3618 ACCGGT A^CCGGT
AhdI 8764 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 187 GGCGCGCC GG^CGCGCC
AspEI 8764 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 7021 GGCGCC GGCGC^C
BbrPI 2300 CACGTG CAC^GTG
BseAI 75 TCCGGA T^CCGGA
BspEI 75 TCCGGA T^CCGGA
BspLU11I 8108 ACATGT A^CATGT
BssHII 188 GCGCGC G^CGCGC
BstEII 1290 GGTTACC G^GT[ACGT]ACC
BstPI 1290 GGTTACC G^GT[ACGT]ACC
DraI 554 TTTAAA TTT^AAA
DrdI 97 GACCAGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 8764 GACTTTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 883 CCTCTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1290 GGTTACC G^GT[ACGT]ACC
EcoRI 6083 GAATTC G^AATTC
EcoT22I 2311 ATGCAT ATGCA^T
HpaI 1709 GTTAAC GTT^AAC
KasI 7021 GGCGCC G^GCGCC
KspI 283 CCGCGG CCGC^GG
MroI 75 TCCGGA T^CCGGA
NarI 7021 GGCGCC GG^CGCC
NdeI 6298 CATATG CA^TATG
NsiI 2311 ATGCAT ATGCA^T
PinAI 3618 ACCGGT A^CCGGT
PmaCI 2300 CACGTG CAC^GTG
PmlI 2300 CACGTG CAC^GTG
PshAI 2353 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 904 GAGCTC GAGCT^C
SacII 283 CCGCGG CCGC^GG
SalI 3118 GTCGAC G^TCGAC
SbfI 2567 CCTGCAGG CCTGCA^GG
ScaI 4956 AGTACT AGT^ACT
SfoI 7021 GGCGCC GGC^GCC
SpeI 4667 ACTAGT A^CTAGT
SphI 3224 GCATGC GCATG^C
Sse8387I 2567 CCTGCAGG CCTGCA^GG
XbaI 7388 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 458 GACGTC GACGT^C
755 GACGTC
AflIII 2301 ACGTGT A^C[GA][CT]GT
8108 ACATGT
AocI 835 CCTTAGG CC^T[ACGT]AGG
9016 CCTAAGG
Asp700I 8030 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
8298 GAACTCATTC
AspI 79 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
8062 GACTATGTC
BalI 2920 TGGCCA TGG^CCA
6503 TGGCCA
BbvCI 5241 GCTGAGG CCTCAGC(-5/-2)
9133 GCTGAGG
BsaAI 1405 TACGTG [CT]AC^GT[GA]
2300 CACGTG
BsaWI 75 TCCGGA [AT]^CCGG[AT]
3618 ACCGGT
BsiEI 147 CGGCCG CG[GA][CT]^CG
203 CGGCCG
BsmBI 8206 CGTCTC CGTCTC(1/5)
3431 GAGACG
BstAPI 5388 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5748 GCAGCAGCTGC
BstXI 5561 CCATGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6595 CCATCCCCTTGG
Bsu36I 835 CCTTAGG CC^T[ACGT]AGG
9016 CCTAAGG
EagI 147 CGGCCG C^GGCCG
203 CGGCCG
EclXI 147 CGGCCG C^GGCCG
203 CGGCCG
Eco52I 147 CGGCCG C^GGCCG
203 CGGCCG
Eco81I 835 CCTTAGG CC^T[ACGT]AGG
9016 CCTAAGG
MluNI 2920 TGGCCA TGG^CCA
6503 TGGCCA
MscI 2920 TGGCCA TGG^CCA
6503 TGGCCA
NaeI 181 GCCGGC GCC^GGC
919 GCCGGC
NgoMIV 181 GCCGGC G^CCGGC
919 GCCGGC
PflFI 79 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
8062 GACTATGTC
PpuMI 2276 GGGTCCT [GA]G^G[AT]CC[CT]
3656 AGGTCCT
SapI 2397 GAAGAGC GCTCTTC(1/4)
4551 GAAGAGC
Tth111I 79 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
8062 GACTATGTC
XmnI 8030 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
8298 GAACTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI Asp718I AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BssSI Bst1107I
BstF5I BstNI BstUI BstYI BstZ17I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NciI NcoI NdeII NheI
NlaIII NlaIV NspI PaeR7I PflMI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII