##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI BamHI
BbvCI BcgI BsaWI BseAI BsiWI
BspDI BspEI BsrDI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoT22I HindIII HpaI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI PvuI
RsrII SacI SalI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1382 GGTACC G^GTACC
AccI 1956 GTCTAC GT^[AC][GT]AC
AclI 2516 AACGTT AA^CGTT
AflII 2179 CTTAAG C^TTAAG
Asp718I 1382 GGTACC G^GTACC
AvrII 3826 CCTAGG C^CTAGG
BciVI 1326 GGATAC GTATCC(6/5)
BfrI 2179 CTTAAG C^TTAAG
BglI 1282 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 726 AGATCT A^GATCT
BlnI 3826 CCTAGG C^CTAGG
BsmI 915 GAATGC GAATGC(1/-1)
Bsp1407I 524 TGTACA T^GTACA
BspHI 207 TCATGA T^CATGA
BspLU11I 3949 ACATGT A^CATGT
BsrGI 524 TGTACA T^GTACA
DraIII 4185 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2584 GACACAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoRV 55 GATATC GAT^ATC
FseI 3820 GGCCGGCC GGCCGG^CC
HincII 2362 GTCAAC GT[CT]^[GA]AC
HindII 2362 GTCAAC GT[CT]^[GA]AC
KpnI 1382 GGTACC GGTAC^C
KspI 2012 CCGCGG CCGC^GG
NcoI 551 CCATGG C^CATGG
PshAI 2177 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2516 AACGTT AA^CGTT
RcaI 207 TCATGA T^CATGA
SacII 2012 CCGCGG CCGC^GG
SbfI 4275 CCTGCAGG CCTGCA^GG
SphI 3727 GCATGC GCATG^C
Sse8387I 4275 CCTGCAGG CCTGCA^GG
SspBI 524 TGTACA T^GTACA
XbaI 3353 TCTAGA T^CTAGA
XcmI 4152 CCATTCTGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 531 GACGTC GACGT^C
711 GACGTC
AocI 454 CCTAAGG CC^T[ACGT]AGG
2822 CCTGAGG
Asp700I 1644 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2556 GAACATCTTC
AviII 1685 TGCGCA TGC^GCA
2759 TGCGCA
BbeI 2394 GGCGCC GGCGC^C
2929 GGCGCC
BbrPI 2984 CACGTG CAC^GTG
3399 CACGTG
BsmBI 3587 CGTCTC CGTCTC(1/5)
1108 GAGACG
BsrBI 1135 GAGCGG CCGCTC(-3/-3)
1816 GAGCGG
BssHII 1610 GCGCGC G^CGCGC
2342 GCGCGC
BstAPI 2544 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4054 GCAGTGTGTGC
Bsu36I 454 CCTAAGG CC^T[ACGT]AGG
2822 CCTGAGG
DraI 130 TTTAAA TTT^AAA
664 TTTAAA
Eco81I 454 CCTAAGG CC^T[ACGT]AGG
2822 CCTGAGG
FspI 1685 TGCGCA TGC^GCA
2759 TGCGCA
KasI 2394 GGCGCC G^GCGCC
2929 GGCGCC
NarI 2394 GGCGCC GG^CGCC
2929 GGCGCC
PflMI 594 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4240 CCAGCTGCTGG
PmaCI 2984 CACGTG CAC^GTG
3399 CACGTG
PmlI 2984 CACGTG CAC^GTG
3399 CACGTG
SexAI 1315 ACCTGGT A^CC[AT]GGT
1481 ACCTGGT
SfoI 2394 GGCGCC GGC^GCC
2929 GGCGCC
TfiI 704 GAATC G^A[AT]TC
2675 GAATC
Van91I 594 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4240 CCAGCTGCTGG
XmnI 1644 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2556 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII