##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspI BanI BbeI BciVI
BclI BlpI Bpu1102I BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI BstBI Bsu36I CelII
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FbaI FseI KasI KpnI MluI
MroI NarI NheI NotI NruI
NspV PacI PflFI PmeI PshAI
Psp1406I PvuI RsrII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspBI
StuI SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 2775 GACGCC G[GA]^CG[CT]C
AflII 3427 CTTAAG C^TTAAG
AgeI 474 ACCGGT A^CCGGT
AhdI 2736 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2736 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 590 TGCGCA TGC^GCA
AvrII 121 CCTAGG C^CTAGG
BamHI 3192 GGATCC G^GATCC
BbrPI 1012 CACGTG CAC^GTG
BcgI 2140 CGAATTTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 227 CCCGG CC^[GC]GG
BfrI 3427 CTTAAG C^TTAAG
BglI 629 GCCGCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 121 CCTAGG C^CTAGG
BsaAI 1012 CACGTG [CT]AC^GT[GA]
BsaBI 1616 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2775 GACGCC G[GA]^CG[CT]C
BsmBI 2773 GAGACG CGTCTC(1/5)
BspHI 2679 TCATGA T^CATGA
BspLU11I 1901 ACATGT A^CATGT
BstEII 887 GGTCACC G^GT[ACGT]ACC
BstPI 887 GGTCACC G^GT[ACGT]ACC
BstXI 786 CCATTATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2658 GACTTCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2736 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 887 GGTCACC G^GT[ACGT]ACC
FspI 590 TGCGCA TGC^GCA
HaeII 2747 AGCGCC [GA]GCGC^[CT]
Hin1I 2775 GACGCC G[GA]^CG[CT]C
HpaI 954 GTTAAC GTT^AAC
KspI 2846 CCGCGG CCGC^GG
MamI 1616 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 278 GCCGGC GCC^GGC
NciI 227 CCCGG CC^[GC]GG
NcoI 455 CCATGG C^CATGG
NgoMIV 278 GCCGGC G^CCGGC
PaeR7I 2999 CTCGAG C^TCGAG
PflMI 1750 CCAACTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 474 ACCGGT A^CCGGT
PmaCI 1012 CACGTG CAC^GTG
PmlI 1012 CACGTG CAC^GTG
RcaI 2679 TCATGA T^CATGA
SacI 421 GAGCTC GAGCT^C
SacII 2846 CCGCGG CCGC^GG
SbfI 2904 CCTGCAGG CCTGCA^GG
SphI 1352 GCATGC GCATG^C
Sse8387I 2904 CCTGCAGG CCTGCA^GG
Van91I 1750 CCAACTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2999 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1013 ACGTGT A^C[GA][CT]GT
1901 ACATGT
Asp700I 1096 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1237 GAAGTGATTC
BalI 636 TGGCCA TGG^CCA
783 TGGCCA
BanII 421 GAGCTC G[GA]GC[CT]^C
3555 GGGCTC
BglII 615 AGATCT A^GATCT
2154 AGATCT
BsaWI 134 ACCGGA [AT]^CCGG[AT]
474 ACCGGT
BspMI 1200 ACCTGC ACCTGC(4/8)
2903 ACCTGC
BsrFI 278 GCCGGC [GA]^CCGG[CT]
474 ACCGGT
Bst1107I 3771 GTATAC GTA^TAC
3940 GTATAC
BstAPI 60 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2461 GCAGGATATGC
BstZ17I 3771 GTATAC GTA^TAC
3940 GTATAC
Cfr10I 278 GCCGGC [GA]^CCGG[CT]
474 ACCGGT
DraI 2323 TTTAAA TTT^AAA
3716 TTTAAA
EcoT14I 121 CCTAGG C^C[AT][AT]GG
455 CCATGG
HgaI 81 GACGC GACGC(5/10)
2775 GACGC
HincII 664 GTTGAC GT[CT]^[GA]AC
954 GTTAAC
HindII 664 GTTGAC GT[CT]^[GA]AC
954 GTTAAC
HindIII 2391 AAGCTT A^AGCTT
3361 AAGCTT
MfeI 962 CAATTG C^AATTG
4337 CAATTG
MluNI 636 TGGCCA TGG^CCA
783 TGGCCA
MscI 636 TGGCCA TGG^CCA
783 TGGCCA
MunI 962 CAATTG C^AATTG
4337 CAATTG
NspI 1352 GCATGC [GA]CATG^[CT]
1901 ACATGT
PvuII 583 CAGCTG CAG^CTG
1105 CAGCTG
StyI 121 CCTAGG C^C[AT][AT]GG
455 CCATGG
XmnI 1096 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1237 GAAGTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaI AvaII BbsI
BbvCI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeI NdeII NlaIII NlaIV NsiI
PleI PpuMI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII