##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I CelII Cfr10I
CpoI DrdI DsaI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT14I
EcoT22I FseI FspI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1777 GACGTC GACGT^C
AccIII 2158 TCCGGA T^CCGGA
AclI 2188 AACGTT AA^CGTT
AcyI 1777 GACGTC G[GA]^CG[CT]C
AflII 1644 CTTAAG C^TTAAG
AflIII 3349 ACATGT A^C[GA][CT]GT
AhdI 666 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1538 CCTGAGG CC^T[ACGT]AGG
AspEI 666 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1547 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 719 CGATGGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1488 GGATAC GTATCC(6/5)
BfrI 1644 CTTAAG C^TTAAG
BglI 1692 GCCTTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1621 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1777 GACGTC G[GA]^CG[CT]C
BsaI 3674 GGTCTC GGTCTC(1/5)
BseAI 2158 TCCGGA T^CCGGA
BsgI 135 GTGCAG GTGCAG(16/14)
BspDI 1618 ATCGAT AT^CGAT
BspEI 2158 TCCGGA T^CCGGA
BspHI 3325 TCATGA T^CATGA
BspLU11I 3349 ACATGT A^CATGT
BsrDI 2667 GCAATG GCAATG(2/0)
BstAPI 3443 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3189 TTCGAA TT^CGAA
Bsu36I 1538 CCTGAGG CC^T[ACGT]AGG
ClaI 1618 ATCGAT AT^CGAT
DraIII 3148 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 666 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1538 CCTGAGG CC^T[ACGT]AGG
EcoNI 2578 CCTTAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1409 GAATTC G^AATTC
HaeII 2485 AGCGCC [GA]GCGC^[CT]
Hin1I 1777 GACGTC G[GA]^CG[CT]C
HincII 3105 GTTAAC GT[CT]^[GA]AC
HindII 3105 GTTAAC GT[CT]^[GA]AC
HindIII 1359 AAGCTT A^AGCTT
HpaI 3105 GTTAAC GTT^AAC
MamI 1621 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2158 TCCGGA T^CCGGA
MslI 831 CATTTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 2672 GCTAGC G^CTAGC
NspV 3189 TTCGAA TT^CGAA
PflFI 1547 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2776 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2400 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2188 AACGTT AA^CGTT
PvuII 1785 CAGCTG CAG^CTG
RcaI 3325 TCATGA T^CATGA
SapI 1770 GAAGAGC GCTCTTC(1/4)
SfuI 3189 TTCGAA TT^CGAA
Tth111I 1547 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 209 GTCTAC GT^[AC][GT]AC
1008 GTAGAC
AvaII 2777 GGACC G^G[AT]CC
3340 GGTCC
BalI 839 TGGCCA TGG^CCA
2857 TGGCCA
BcnI 398 CCCGG CC^[GC]GG
2654 CCGGG
BsaWI 2158 TCCGGA [AT]^CCGG[AT]
3337 TCCGGT
BsmFI 2776 GGGAC GGGAC(10/14)
3561 GGGAC
BsmI 1425 GAATGC GAATGC(1/-1)
3693 GAATGC
DraII 394 AGGCCCC [GA]G^G[ACGT]CC[CT]
2776 GGGACCT
EaeI 839 TGGCCA [CT]^GGCC[GA]
2857 TGGCCA
EcoO109I 394 AGGCCCC [GA]G^G[ACGT]CC[CT]
2776 GGGACCT
HgaI 2713 GACGC GACGC(5/10)
2784 GACGC
MaeII 1778 ACGT A^CGT
2189 ACGT
MluNI 839 TGGCCA TGG^CCA
2857 TGGCCA
MscI 839 TGGCCA TGG^CCA
2857 TGGCCA
MspA1I 998 CAGCGG C[AC]G^C[GT]G
1785 CAGCTG
NciI 398 CCCGG CC^[GC]GG
2654 CCGGG
NspI 1529 ACATGC [GA]CATG^[CT]
3349 ACATGT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
BanII BbsI BbvI BclI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI