##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccI AccII AccIII AflII
Alw44I AocI ApaI ApaLI AscI
AseI AsnI AspI AvaI AviII
AvrII BamHI BbrPI BcgI BclI
BfrI BlnI BsaAI BsaBI BseAI
BsiEI BsmBI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII Bst1107I
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoRI FauI FbaI FseI
FspI HpaI KspI MamI MluI
MroI MvnI NaeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 3821 AACGTT AA^CGTT
AcyI 68 GGCGCC G[GA]^CG[CT]C
AflIII 4087 ACATGT A^C[GA][CT]GT
AhdI 3466 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3761 AGCGCT AGC^GCT
AspEI 3466 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 68 GGCGCC GGCGC^C
BbsI 3525 GTCTTC GAAGAC(2/6)
BglII 1872 AGATCT A^GATCT
BpuAI 3525 GTCTTC GAAGAC(2/6)
BsaHI 68 GGCGCC G[GA]^CG[CT]C
BsiWI 2786 CGTACG C^GTACG
BspHI 3449 TCATGA T^CATGA
BspLU11I 4087 ACATGT A^CATGT
BssSI 1832 CTCGTG CACGAG(-5/-1)
BstBI 1547 TTCGAA TT^CGAA
Eam1105I 3466 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3761 AGCGCT AGC^GCT
EcoNI 2755 CCTGTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 3331 GCGTC GACGC(5/10)
Hin1I 68 GGCGCC G[GA]^CG[CT]C
HindIII 1420 AAGCTT A^AGCTT
KasI 68 GGCGCC G^GCGCC
NarI 68 GGCGCC GG^CGCC
NspV 1547 TTCGAA TT^CGAA
PpuMI 3345 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 3821 AACGTT AA^CGTT
RcaI 3449 TCATGA T^CATGA
SacI 3254 GAGCTC GAGCT^C
SapI 1984 GAAGAGC GCTCTTC(1/4)
ScaI 2596 AGTACT AGT^ACT
SfoI 68 GGCGCC GGC^GCC
SfuI 1547 TTCGAA TT^CGAA
SpeI 2593 ACTAGT A^CTAGT
SphI 3599 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 581 GGTACC G^GTACC
2979 GGTACC
AgeI 24 ACCGGT A^CCGGT
1085 ACCGGT
Asp700I 768 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1610 GAATGCCTTC
Asp718I 581 GGTACC G^GTACC
2979 GGTACC
BbvCI 1692 CCTCAGC CCTCAGC(-5/-2)
3061 CCTCAGC
BsrFI 24 ACCGGT [GA]^CCGG[CT]
1085 ACCGGT
BstAPI 1716 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3675 GCAGTCTTTGC
BstEII 1088 GGTTACC G^GT[ACGT]ACC
3463 GGTGACC
BstPI 1088 GGTTACC G^GT[ACGT]ACC
3463 GGTGACC
CfoI 69 GCGC GCG^C
3762 GCGC
Cfr10I 24 ACCGGT [GA]^CCGG[CT]
1085 ACCGGT
DraII 600 GGGGCCT [GA]G^G[ACGT]CC[CT]
3345 AGGTCCT
EcoO109I 600 GGGGCCT [GA]G^G[ACGT]CC[CT]
3345 AGGTCCT
EcoO65I 1088 GGTTACC G^GT[ACGT]ACC
3463 GGTGACC
EcoRV 189 GATATC GAT^ATC
886 GATATC
HaeII 68 GGCGCC [GA]GCGC^[CT]
3761 AGCGCT
HhaI 69 GCGC GCG^C
3762 GCGC
HinP1I 69 GCGC G^CGC
3762 GCGC
HincII 1414 GTTGAC GT[CT]^[GA]AC
2134 GTTGAC
HindII 1414 GTTGAC GT[CT]^[GA]AC
2134 GTTGAC
KpnI 581 GGTACC GGTAC^C
2979 GGTACC
MaeII 1594 ACGT A^CGT
3822 ACGT
MfeI 472 CAATTG C^AATTG
1172 CAATTG
MunI 472 CAATTG C^AATTG
1172 CAATTG
NdeI 822 CATATG CA^TATG
2965 CATATG
PflMI 956 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2824 CCAGTTGCTGG
PinAI 24 ACCGGT A^CCGGT
1085 ACCGGT
Van91I 956 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2824 CCAGTTGCTGG
XmnI 768 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1610 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbvI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraIII DsaI EaeI EarI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII