##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AviII
BanII BbrPI BclI BfrI BglI
BsaAI BseAI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BstBI ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI FspI HgaI HpaI KpnI
MfeI MluI MroI MunI NotI
NruI NspV PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 3073 AACGTT AA^CGTT
Asp700I 1929 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3420 CCTAGG C^CTAGG
BamHI 3979 GGATCC G^GATCC
BcgI 1923 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1740 CCGGG CC^[GC]GG
BlnI 3420 CCTAGG C^CTAGG
BlpI 2712 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2712 GCTGAGC GC^T[ACGT]AGC
BsaBI 3902 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 553 ACCGGA [AT]^CCGG[AT]
BsiEI 19 CGGTCG CG[GA][CT]^CG
BspHI 454 TCATGA T^CATGA
BspLU11I 369 ACATGT A^CATGT
BsrFI 1211 GCCGGC [GA]^CCGG[CT]
BssSI 3384 CACGAG CACGAG(-5/-1)
CelII 2712 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1211 GCCGGC [GA]^CCGG[CT]
HincII 2573 GTCGAC GT[CT]^[GA]AC
HindII 2573 GTCGAC GT[CT]^[GA]AC
KspI 31 CCGCGG CCGC^GG
MamI 3902 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1211 GCCGGC GCC^GGC
NciI 1740 CCGGG CC^[GC]GG
NcoI 1667 CCATGG C^CATGG
NdeI 1479 CATATG CA^TATG
NgoMIV 1211 GCCGGC G^CCGGC
NheI 1278 GCTAGC G^CTAGC
PacI 3149 TTAATTAA TTAAT^TAA
PpuMI 1650 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 3073 AACGTT AA^CGTT
RcaI 454 TCATGA T^CATGA
SacII 31 CCGCGG CCGC^GG
SalI 2573 GTCGAC G^TCGAC
XmnI 1929 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 32 CGCG CG^CG
41 CGCG
AcyI 64 GGCGCC G[GA]^CG[CT]C
1889 GGCGCC
AvaI 388 CTCGAG C^[CT]CG[GA]G
442 CTCGAG
BalI 1828 TGGCCA TGG^CCA
2103 TGGCCA
BbeI 64 GGCGCC GGCGC^C
1889 GGCGCC
BbsI 837 GTCTTC GAAGAC(2/6)
4105 GTCTTC
BbvCI 1134 CCTCAGC CCTCAGC(-5/-2)
2305 GCTGAGG
BciVI 1458 GGATAC GTATCC(6/5)
3359 GGATAC
BglII 3901 AGATCT A^GATCT
4215 AGATCT
BpuAI 837 GTCTTC GAAGAC(2/6)
4105 GTCTTC
BsaHI 64 GGCGCC G[GA]^CG[CT]C
1889 GGCGCC
BsgI 1266 GTGCAG GTGCAG(16/14)
1528 GTGCAG
BsmBI 2234 CGTCTC CGTCTC(1/5)
1409 GAGACG
BsoBI 388 CTCGAG C^[CT]CG[GA]G
442 CTCGAG
Bst1107I 2449 GTATAC GTA^TAC
2522 GTATAC
BstAPI 1580 GCAGTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1949 GCAGAGGTTGC
BstUI 32 CGCG CG^CG
41 CGCG
BstZ17I 2449 GTATAC GTA^TAC
2522 GTATAC
DraII 1650 GGGACCT [GA]G^G[ACGT]CC[CT]
3501 GGGGCCT
DsaI 31 CCGCGG C^C[GA][CT]GG
1667 CCATGG
EcoO109I 1650 GGGACCT [GA]G^G[ACGT]CC[CT]
3501 GGGGCCT
HaeII 64 GGCGCC [GA]GCGC^[CT]
1889 GGCGCC
Hin1I 64 GGCGCC G[GA]^CG[CT]C
1889 GGCGCC
KasI 64 GGCGCC G^GCGCC
1889 GGCGCC
MluNI 1828 TGGCCA TGG^CCA
2103 TGGCCA
MscI 1828 TGGCCA TGG^CCA
2103 TGGCCA
MvnI 32 CGCG CG^CG
41 CGCG
NarI 64 GGCGCC GG^CGCC
1889 GGCGCC
PaeR7I 388 CTCGAG C^TCGAG
442 CTCGAG
SexAI 328 ACCTGGT A^CC[AT]GGT
3728 ACCTGGT
SfoI 64 GGCGCC GGC^GCC
1889 GGCGCC
SphI 2276 GCATGC GCATG^C
3475 GCATGC
StuI 299 AGGCCT AGG^CCT
935 AGGCCT
XhoI 388 CTCGAG C^TCGAG
442 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspI AvaII BanI
BbvI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
Eco81I EcoNI EcoO65I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PflFI PflMI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XhoII