##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AgeI ApoI AscI AseI AsnI
Asp718I BamHI BbrPI BbvCI BlpI
Bpu1102I BsaBI BsiWI BspDI BspLU11I
BsrDI BssHII Bst1107I BstAPI BstZ17I
CelII ClaI CpoI DraI DrdI
EcoNI EcoRI EcoRV EcoT22I FseI
HpaI KpnI MamI MfeI MunI
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI SgrAI SpeI SphI Sse8387I
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 3375 CTTAAG C^TTAAG
AhdI 1980 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 528 GTGCAC G^TGCAC
Aor51HI 3334 AGCGCT AGC^GCT
ApaLI 528 GTGCAC G^TGCAC
Asp700I 3675 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1980 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4096 TGCGCA TGC^GCA
AvrII 1146 CCTAGG C^CTAGG
BciVI 4064 GTATCC GTATCC(6/5)
BfrI 3375 CTTAAG C^TTAAG
BglII 148 AGATCT A^GATCT
BlnI 1146 CCTAGG C^CTAGG
BsaAI 4237 TACGTA [CT]AC^GT[GA]
BsmI 2449 GCATTC GAATGC(1/-1)
Bsp1407I 4288 TGTACA T^GTACA
BspHI 3613 TCATGA T^CATGA
BsrGI 4288 TGTACA T^GTACA
BstBI 2136 TTCGAA TT^CGAA
BstYI 148 AGATCT [GA]^GATC[CT]
DraIII 717 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4353 CGGCCG C^GGCCG
Eam1105I 1980 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4353 CGGCCG C^GGCCG
Eco47III 3334 AGCGCT AGC^GCT
Eco52I 4353 CGGCCG C^GGCCG
FspI 4096 TGCGCA TGC^GCA
HincII 2337 GTTGAC GT[CT]^[GA]AC
HindII 2337 GTTGAC GT[CT]^[GA]AC
HindIII 2315 AAGCTT A^AGCTT
MflI 148 AGATCT [GA]^GATC[CT]
MslI 1808 CATCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2210 CCATGG C^CATGG
NdeI 1264 CATATG CA^TATG
NspI 4145 GCATGT [GA]CATG^[CT]
NspV 2136 TTCGAA TT^CGAA
PaeR7I 1039 CTCGAG C^TCGAG
PshAI 2595 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3613 TCATGA T^CATGA
ScaI 1968 AGTACT AGT^ACT
SexAI 718 ACCTGGT A^CC[AT]GGT
SfiI 55 GGCCCCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2136 TTCGAA TT^CGAA
SnaBI 4237 TACGTA TAC^GTA
SspBI 4288 TGTACA T^GTACA
Tsp509I 4441 AATT ^AATT
XhoI 1039 CTCGAG C^TCGAG
XhoII 148 AGATCT [GA]^GATC[CT]
XmnI 3675 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 2008 TCCGGA T^CCGGA
3963 TCCGGA
AflIII 4050 ACGCGT A^C[GA][CT]GT
4246 ACGCGT
AocI 59 CCTCAGG CC^T[ACGT]AGG
4212 CCTCAGG
ApaI 1908 GGGCCC GGGCC^C
2811 GGGCCC
BbeI 3738 GGCGCC GGCGC^C
4332 GGCGCC
BbsI 389 GAAGAC GAAGAC(2/6)
178 GTCTTC
BcgI 4005 GCACCCGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4099 GCACGCCCCTCG
BclI 250 TGATCA T^GATCA
1802 TGATCA
BglI 56 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1115 GCCCACCAGGC
BpuAI 389 GAAGAC GAAGAC(2/6)
178 GTCTTC
BsaI 2280 GAGACC GGTCTC(1/5)
3036 GAGACC
BseAI 2008 TCCGGA T^CCGGA
3963 TCCGGA
BsiEI 602 CGACCG CG[GA][CT]^CG
4353 CGGCCG
BspEI 2008 TCCGGA T^CCGGA
3963 TCCGGA
BsrFI 3289 GCCGGC [GA]^CCGG[CT]
4393 GCCGGC
BstEII 71 GGTGACC G^GT[ACGT]ACC
360 GGTTACC
BstPI 71 GGTGACC G^GT[ACGT]ACC
360 GGTTACC
Bsu36I 59 CCTCAGG CC^T[ACGT]AGG
4212 CCTCAGG
Cfr10I 3289 GCCGGC [GA]^CCGG[CT]
4393 GCCGGC
Eco81I 59 CCTCAGG CC^T[ACGT]AGG
4212 CCTCAGG
EcoO65I 71 GGTGACC G^GT[ACGT]ACC
360 GGTTACC
FbaI 250 TGATCA T^GATCA
1802 TGATCA
KasI 3738 GGCGCC G^GCGCC
4332 GGCGCC
KspI 18 CCGCGG CCGC^GG
4336 CCGCGG
MluI 4050 ACGCGT A^CGCGT
4246 ACGCGT
MroI 2008 TCCGGA T^CCGGA
3963 TCCGGA
NaeI 3289 GCCGGC GCC^GGC
4393 GCCGGC
NarI 3738 GGCGCC GG^CGCC
4332 GGCGCC
NgoMIV 3289 GCCGGC G^CCGGC
4393 GCCGGC
PstI 572 CTGCAG CTGCA^G
782 CTGCAG
SacI 942 GAGCTC GAGCT^C
1452 GAGCTC
SacII 18 CCGCGG CCGC^GG
4336 CCGCGG
SfoI 3738 GGCGCC GGC^GCC
4332 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AspI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI Tth111I TthHB8I Van91I
XcmI XmaCI XmaI