##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
ApaI AscI Asp718I AspEI AvrII
BalI BbeI BclI BlnI BsmBI
BspHI BsrFI BssHII Cfr10I CpoI
DraIII EagI Eam1105I EclXI Eco52I
FbaI FseI KasI KpnI KspI
MluI MluNI MscI NaeI NarI
NcoI NgoMIV NotI NruI PacI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 129 TCCGGA T^CCGGA
AflII 974 CTTAAG C^TTAAG
AocI 3740 CCTCAGG CC^T[ACGT]AGG
Aor51HI 5605 AGCGCT AGC^GCT
AvaI 1587 CTCGAG C^[CT]CG[GA]G
AviII 541 TGCGCA TGC^GCA
BamHI 148 GGATCC G^GATCC
BanI 4688 GGCACC G^G[CT][GA]CC
BbrPI 2626 CACGTG CAC^GTG
BbvCI 3188 CCTCAGC CCTCAGC(-5/-2)
BcgI 1247 CGAACAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3581 GGATAC GTATCC(6/5)
BfrI 974 CTTAAG C^TTAAG
BsaAI 2626 CACGTG [CT]AC^GT[GA]
BsaWI 129 TCCGGA [AT]^CCGG[AT]
BseAI 129 TCCGGA T^CCGGA
BsiEI 3399 CGGTCG CG[GA][CT]^CG
BsiWI 5702 CGTACG C^GTACG
BsoBI 1587 CTCGAG C^[CT]CG[GA]G
BspEI 129 TCCGGA T^CCGGA
BspLU11I 5255 ACATGT A^CATGT
BsrBI 83 GAGCGG CCGCTC(-3/-3)
BssSI 3612 CTCGTG CACGAG(-5/-1)
BstBI 1245 TTCGAA TT^CGAA
Bsu36I 3740 CCTCAGG CC^T[ACGT]AGG
DrdI 1630 GACATTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 21 CCACGG C^C[GA][CT]GG
EaeI 12 CGGCCA [CT]^GGCC[GA]
Eco47III 5605 AGCGCT AGC^GCT
Eco81I 3740 CCTCAGG CC^T[ACGT]AGG
EcoRI 478 GAATTC G^AATTC
EcoT22I 282 ATGCAT ATGCA^T
FspI 541 TGCGCA TGC^GCA
HaeII 5605 AGCGCT [GA]GCGC^[CT]
MroI 129 TCCGGA T^CCGGA
NheI 1312 GCTAGC G^CTAGC
NsiI 282 ATGCAT ATGCA^T
NspV 1245 TTCGAA TT^CGAA
PaeR7I 1587 CTCGAG C^TCGAG
PflMI 2780 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2626 CACGTG CAC^GTG
PmlI 2626 CACGTG CAC^GTG
SexAI 749 ACCAGGT A^CC[AT]GGT
SfuI 1245 TTCGAA TT^CGAA
StuI 2687 AGGCCT AGG^CCT
Van91I 2780 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1587 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 42 GACGCC G[GA]^CG[CT]C
2012 GGCGTC
AflIII 2625 ACACGT A^C[GA][CT]GT
5255 ACATGT
Alw44I 3348 GTGCAC G^TGCAC
4863 GTGCAC
ApaLI 3348 GTGCAC G^TGCAC
4863 GTGCAC
AseI 5410 ATTAAT AT^TAAT
5961 ATTAAT
AsnI 5410 ATTAAT AT^TAAT
5961 ATTAAT
AspI 2525 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3531 GACGCAGTC
BglI 30 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4724 GCCTCTAAGGC
BlpI 2663 GCTGAGC GC^T[ACGT]AGC
4049 GCTCAGC
Bpu1102I 2663 GCTGAGC GC^T[ACGT]AGC
4049 GCTCAGC
BsaBI 443 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2576 GATCTAGATC
BsaHI 42 GACGCC G[GA]^CG[CT]C
2012 GGCGTC
BsaI 3433 GAGACC GGTCTC(1/5)
2800 GGTCTC
BsmI 1894 GAATGC GAATGC(1/-1)
2133 GAATGC
BspDI 431 ATCGAT AT^CGAT
2312 ATCGAT
Bst1107I 5267 GTATAC GTA^TAC
5713 GTATAC
BstEII 2440 GGTAACC G^GT[ACGT]ACC
4486 GGTAACC
BstPI 2440 GGTAACC G^GT[ACGT]ACC
4486 GGTAACC
BstZ17I 5267 GTATAC GTA^TAC
5713 GTATAC
CelII 2663 GCTGAGC GC^T[ACGT]AGC
4049 GCTCAGC
ClaI 431 ATCGAT AT^CGAT
2312 ATCGAT
DraII 3283 GGGTCCT [GA]G^G[ACGT]CC[CT]
3571 AGGACCT
EcoNI 3548 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6655 CCTTTATCAGG
EcoO109I 3283 GGGTCCT [GA]G^G[ACGT]CC[CT]
3571 AGGACCT
EcoO65I 2440 GGTAACC G^GT[ACGT]ACC
4486 GGTAACC
Hin1I 42 GACGCC G[GA]^CG[CT]C
2012 GGCGTC
MamI 443 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2576 GATCTAGATC
MfeI 1866 CAATTG C^AATTG
5135 CAATTG
MunI 1866 CAATTG C^AATTG
5135 CAATTG
PflFI 2525 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3531 GACGCAGTC
PpuMI 3283 GGGTCCT [GA]G^G[AT]CC[CT]
3571 AGGACCT
PshBI 5410 ATTAAT AT^TAAT
5961 ATTAAT
SacI 1440 GAGCTC GAGCT^C
2339 GAGCTC
Tth111I 2525 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3531 GACGCAGTC
XbaI 1183 TCTAGA T^CTAGA
2578 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SpeI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI