##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
BbrPI BfrI BsaAI BsiWI BsmBI
BspDI BspHI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
DrdI EcoRV EcoT22I FseI HgaI
HincII HindII HpaI KpnI KspI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SspI
SwaI Tru9I XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 1349 GACGTC GACGT^C
AccII 2035 CGCG CG^CG
AccIII 2165 TCCGGA T^CCGGA
AhdI 3028 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 242 GTGCAC G^TGCAC
AocI 3525 CCTGAGG CC^T[ACGT]AGG
ApaI 3904 GGGCCC GGGCC^C
ApaLI 242 GTGCAC G^TGCAC
AspEI 3028 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1759 TGCGCA TGC^GCA
AvrII 2955 CCTAGG C^CTAGG
BamHI 2168 GGATCC G^GATCC
BbeI 1969 GGCGCC GGCGC^C
BcgI 1846 GCAAGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1295 GGATAC GTATCC(6/5)
BclI 1439 TGATCA T^GATCA
BglI 1821 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2955 CCTAGG C^CTAGG
BsaBI 969 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2165 TCCGGA [AT]^CCGG[AT]
BseAI 2165 TCCGGA T^CCGGA
BsiEI 2753 CGGCCG CG[GA][CT]^CG
Bsp1407I 3186 TGTACA T^GTACA
BspEI 2165 TCCGGA T^CCGGA
BsrFI 1678 GCCGGC [GA]^CCGG[CT]
BsrGI 3186 TGTACA T^GTACA
BstUI 2035 CGCG CG^CG
Bsu36I 3525 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1678 GCCGGC [GA]^CCGG[CT]
DsaI 469 CCATGG C^C[GA][CT]GG
EagI 2753 CGGCCG C^GGCCG
Eam1105I 3028 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2753 CGGCCG C^GGCCG
Eco52I 2753 CGGCCG C^GGCCG
Eco81I 3525 CCTGAGG CC^T[ACGT]AGG
FbaI 1439 TGATCA T^GATCA
FspI 1759 TGCGCA TGC^GCA
HindIII 2245 AAGCTT A^AGCTT
KasI 1969 GGCGCC G^GCGCC
MamI 969 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2165 TCCGGA T^CCGGA
MvnI 2035 CGCG CG^CG
NaeI 1678 GCCGGC GCC^GGC
NarI 1969 GGCGCC GG^CGCC
NcoI 469 CCATGG C^CATGG
NgoMIV 1678 GCCGGC G^CCGGC
PaeR7I 2547 CTCGAG C^TCGAG
SbfI 1109 CCTGCAGG CCTGCA^GG
SfoI 1969 GGCGCC GGC^GCC
SmaI 3913 CCCGGG CCC^GGG
SpeI 3092 ACTAGT A^CTAGT
Sse8387I 1109 CCTGCAGG CCTGCA^GG
SspBI 3186 TGTACA T^GTACA
StuI 3922 AGGCCT AGG^CCT
XbaI 312 TCTAGA T^CTAGA
XhoI 2547 CTCGAG C^TCGAG
XmaCI 3913 CCCGGG C^CCGGG
XmaI 3913 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1977 GAATTC [GA]^AATT[CT]
3152 GAATTC
AcyI 1349 GACGTC G[GA]^CG[CT]C
1969 GGCGCC
Aor51HI 1866 AGCGCT AGC^GCT
2269 AGCGCT
ApoI 1977 GAATTC [GA]^AATT[CT]
3152 GAATTC
AspI 2392 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3964 GACACTGTC
AvaI 2547 CTCGAG C^[CT]CG[GA]G
3913 CCCGGG
BbsI 2902 GAAGAC GAAGAC(2/6)
1288 GTCTTC
BglII 968 AGATCT A^GATCT
3594 AGATCT
BpuAI 2902 GAAGAC GAAGAC(2/6)
1288 GTCTTC
BsaHI 1349 GACGTC G[GA]^CG[CT]C
1969 GGCGCC
BsmI 1817 GAATGC GAATGC(1/-1)
3231 GCATTC
BsoBI 2547 CTCGAG C^[CT]CG[GA]G
3913 CCCGGG
BspLU11I 2617 ACATGT A^CATGT
3189 ACATGT
BsrDI 3057 GCAATG GCAATG(2/0)
3456 GCAATG
BssSI 922 CTCGTG CACGAG(-5/-1)
2703 CTCGTG
BstEII 445 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
BstPI 445 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
BstXI 3473 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4042 CCATGACATTGG
DraIII 1250 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1566 CACGCTGTG
Eco47III 1866 AGCGCT AGC^GCT
2269 AGCGCT
EcoO65I 445 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
EcoRI 1977 GAATTC G^AATTC
3152 GAATTC
Hin1I 1349 GACGTC G[GA]^CG[CT]C
1969 GGCGCC
PflFI 2392 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3964 GACACTGTC
PvuII 268 CAGCTG CAG^CTG
2481 CAGCTG
SacI 735 GAGCTC GAGCT^C
3355 GAGCTC
Tth111I 2392 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3964 GACACTGTC
XcmI 1282 CCAGCGGTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2662 CCATTCATCCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII