##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
AspEI AspI AviII AvrII BamHI
BbeI BcgI BfrI BlnI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BspEI BsrBI BssHII BstBI BstUI
CelII CpoI DrdI EagI Eam1105I
EclXI Eco52I FseI FspI KasI
KspI MluI MroI MvnI NarI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PvuI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI StuI SwaI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 1994 GGTACC G^GTACC
AclI 1676 AACGTT AA^CGTT
AcyI 2864 GACGCC G[GA]^CG[CT]C
Aor51HI 2899 AGCGCT AGC^GCT
AseI 3041 ATTAAT AT^TAAT
AsnI 3041 ATTAAT AT^TAAT
Asp700I 3806 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1994 GGTACC G^GTACC
BbrPI 989 CACGTG CAC^GTG
BbvCI 3275 GCTGAGG CCTCAGC(-5/-2)
BciVI 3772 GGATAC GTATCC(6/5)
BcnI 522 CCGGG CC^[GC]GG
BglI 1253 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 2864 GACGCC G[GA]^CG[CT]C
BsaI 2238 GGTCTC GGTCTC(1/5)
BsmI 3483 GCATTC GAATGC(1/-1)
BspDI 2979 ATCGAT AT^CGAT
BsrFI 1683 GCCGGC [GA]^CCGG[CT]
BstAPI 2809 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1768 GGTAACC G^GT[ACGT]ACC
BstPI 1768 GGTAACC G^GT[ACGT]ACC
CfoI 2900 GCGC GCG^C
Cfr10I 1683 GCCGGC [GA]^CCGG[CT]
ClaI 2979 ATCGAT AT^CGAT
DraIII 1662 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3949 CCATGG C^C[GA][CT]GG
Eco47III 2899 AGCGCT AGC^GCT
EcoNI 1711 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1768 GGTAACC G^GT[ACGT]ACC
EcoRI 2615 GAATTC G^AATTC
HaeII 2899 AGCGCT [GA]GCGC^[CT]
HhaI 2900 GCGC GCG^C
Hin1I 2864 GACGCC G[GA]^CG[CT]C
HinP1I 2900 GCGC G^CGC
HindIII 2163 AAGCTT A^AGCTT
HpaI 104 GTTAAC GTT^AAC
KpnI 1994 GGTACC GGTAC^C
MfeI 4034 CAATTG C^AATTG
MunI 4034 CAATTG C^AATTG
NaeI 1683 GCCGGC GCC^GGC
NciI 522 CCGGG CC^[GC]GG
NcoI 3949 CCATGG C^CATGG
NdeI 1448 CATATG CA^TATG
NgoMIV 1683 GCCGGC G^CCGGC
PflMI 3572 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 989 CACGTG CAC^GTG
PmeI 3699 GTTTAAAC GTTT^AAAC
PmlI 989 CACGTG CAC^GTG
PpuMI 3517 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 144 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3041 ATTAAT AT^TAAT
Psp1406I 1676 AACGTT AA^CGTT
SacI 3311 GAGCTC GAGCT^C
SapI 2804 GAAGAGC GCTCTTC(1/4)
SbfI 3512 CCTGCAGG CCTGCA^GG
ScaI 3052 AGTACT AGT^ACT
SmlI 1317 CTCAAG C^T[CT][GA]AG
Sse8387I 3512 CCTGCAGG CCTGCA^GG
Van91I 3572 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 253 TCTAGA T^CTAGA
XmnI 3806 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 1428 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
3408 CAGTGGCTG
BanI 1994 GGTACC G^G[CT][GA]CC
3323 GGTGCC
BbsI 11 GTCTTC GAAGAC(2/6)
2501 GTCTTC
BclI 661 TGATCA T^GATCA
2302 TGATCA
BglII 1006 AGATCT A^GATCT
2106 AGATCT
BpuAI 11 GTCTTC GAAGAC(2/6)
2501 GTCTTC
BsaAI 989 CACGTG [CT]AC^GT[GA]
2312 CACGTA
BsaBI 3009 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3296 GATTATGATC
BseRI 1618 CTCCTC GAGGAG(10/8)
2326 CTCCTC
BssSI 75 CACGAG CACGAG(-5/-1)
2644 CACGAG
DraII 282 AGGCCCT [GA]G^G[ACGT]CC[CT]
3517 AGGTCCC
EcoO109I 282 AGGCCCT [GA]G^G[ACGT]CC[CT]
3517 AGGTCCC
EcoRV 1856 GATATC GAT^ATC
1865 GATATC
EcoT14I 2993 CCAAGG C^C[AT][AT]GG
3949 CCATGG
FauI 972 CCCGC CCCGC(4/6)
2787 GCGGG
FbaI 661 TGATCA T^GATCA
2302 TGATCA
HapII 522 CCGG C^CGG
1684 CCGG
HgaI 2764 GACGC GACGC(5/10)
2864 GACGC
HpaII 522 CCGG C^CGG
1684 CCGG
MamI 3009 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3296 GATTATGATC
MspI 522 CCGG C^CGG
1684 CCGG
SspI 707 AATATT AAT^ATT
4065 AATATT
StyI 2993 CCAAGG C^C[AT][AT]GG
3949 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AspHI
AvaI AvaII BalI BanII BbvI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI Bst1107I BstF5I BstNI BstXI
BstYI BstZ17I Bsu36I Cac8I Cfr13I
DdeI DpnII DraI EaeI EarI
Eco81I EcoRII EcoT22I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII