##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
BbeI BcgI BciVI BclI BfrI
BlpI Bpu1102I BsiEI BsiWI BsmBI
BspDI BspHI BspLU11I BssHII Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoO65I FbaI FseI
HindIII HpaI KasI KspI MfeI
MluI MunI MvnI NarI NdeI
NheI NotI NruI PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1869 GGTACC G^GTACC
AccIII 48 TCCGGA T^CCGGA
AcsI 2028 GAATTC [GA]^AATT[CT]
AcyI 21 GGCGTC G[GA]^CG[CT]C
AflIII 324 ACACGT A^C[GA][CT]GT
Alw44I 116 GTGCAC G^TGCAC
AocI 1554 CCTGAGG CC^T[ACGT]AGG
Aor51HI 461 AGCGCT AGC^GCT
ApaI 2225 GGGCCC GGGCC^C
ApaLI 116 GTGCAC G^TGCAC
ApoI 2028 GAATTC [GA]^AATT[CT]
Asp700I 283 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1869 GGTACC G^GTACC
AspI 3133 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1416 TGCGCA TGC^GCA
AvrII 961 CCTAGG C^CTAGG
BbrPI 589 CACGTG CAC^GTG
BglI 252 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2905 AGATCT A^GATCT
BlnI 961 CCTAGG C^CTAGG
BsaHI 21 GGCGTC G[GA]^CG[CT]C
BseAI 48 TCCGGA T^CCGGA
BsgI 395 CTGCAC GTGCAG(16/14)
BsmI 1383 GCATTC GAATGC(1/-1)
Bsp1407I 2803 TGTACA T^GTACA
BspEI 48 TCCGGA T^CCGGA
BsrGI 2803 TGTACA T^GTACA
BssSI 310 CTCGTG CACGAG(-5/-1)
BstBI 1314 TTCGAA TT^CGAA
Bsu36I 1554 CCTGAGG CC^T[ACGT]AGG
DraI 2671 TTTAAA TTT^AAA
DrdI 473 GACTACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2992 CCATGG C^C[GA][CT]GG
Eco47III 461 AGCGCT AGC^GCT
Eco81I 1554 CCTGAGG CC^T[ACGT]AGG
EcoNI 2543 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2028 GAATTC G^AATTC
EcoRV 272 GATATC GAT^ATC
EcoT22I 2965 ATGCAT ATGCA^T
FspI 1416 TGCGCA TGC^GCA
HgaI 22 GCGTC GACGC(5/10)
Hin1I 21 GGCGTC G[GA]^CG[CT]C
KpnI 1869 GGTACC GGTAC^C
MroI 48 TCCGGA T^CCGGA
NcoI 2992 CCATGG C^CATGG
NsiI 2965 ATGCAT ATGCA^T
NspV 1314 TTCGAA TT^CGAA
PaeR7I 1576 CTCGAG C^TCGAG
PflFI 3133 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1630 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 589 CACGTG CAC^GTG
PmlI 589 CACGTG CAC^GTG
SfuI 1314 TTCGAA TT^CGAA
SspBI 2803 TGTACA T^GTACA
Tth111I 3133 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1630 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1576 CTCGAG C^TCGAG
XmnI 283 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 1631 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2559 CAGGGTCTG
AvaI 350 CCCGAG C^[CT]CG[GA]G
1576 CTCGAG
BalI 69 TGGCCA TGG^CCA
156 TGGCCA
BamHI 96 GGATCC G^GATCC
1773 GGATCC
BanI 432 GGCACC G^G[CT][GA]CC
1869 GGTACC
BsaAI 589 CACGTG [CT]AC^GT[GA]
2632 CACGTA
BsaBI 97 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2288 GATGCCAATC
BsaI 1640 GAGACC GGTCTC(1/5)
721 GGTCTC
BsaWI 48 TCCGGA [AT]^CCGG[AT]
882 TCCGGT
BsoBI 350 CCCGAG C^[CT]CG[GA]G
1576 CTCGAG
BspMI 2981 ACCTGC ACCTGC(4/8)
2212 GCAGGT
DraIII 586 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
665 CACAGAGTG
HaeII 382 GGCGCT [GA]GCGC^[CT]
461 AGCGCT
MamI 97 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2288 GATGCCAATC
MluNI 69 TGGCCA TGG^CCA
156 TGGCCA
MscI 69 TGGCCA TGG^CCA
156 TGGCCA
NaeI 408 GCCGGC GCC^GGC
1650 GCCGGC
NgoMIV 408 GCCGGC G^CCGGC
1650 GCCGGC
NspI 552 GCATGT [GA]CATG^[CT]
846 ACATGC
PshAI 715 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2556 GACCAGGGTC
SacI 1307 GAGCTC GAGCT^C
1849 GAGCTC
SmlI 1034 CTCAAG C^T[CT][GA]AG
1576 CTCGAG
StuI 958 AGGCCT AGG^CCT
2496 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI AvaII BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII