##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccIII AflIII AgeI
Alw44I ApaI ApaLI AscI Asp718I
AvrII BbrPI BglI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BspEI BspLU11I BsrFI BstAPI Cfr10I
CpoI EagI EclXI Eco52I FseI
HindIII HpaI KpnI MluI MroI
NaeI NgoMIV NheI NotI NruI
PacI PaeR7I PflMI PinAI PmaCI
PmlI PshAI PvuI RsrII SalI
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 3700 AACGTT AA^CGTT
AflII 926 CTTAAG C^TTAAG
AseI 3761 ATTAAT AT^TAAT
AsnI 3761 ATTAAT AT^TAAT
AspI 4141 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4249 TGCGCA TGC^GCA
BalI 5115 TGGCCA TGG^CCA
BamHI 4774 GGATCC G^GATCC
BbvCI 262 CCTCAGC CCTCAGC(-5/-2)
BcgI 2471 CGAAGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1930 GGATAC GTATCC(6/5)
BfrI 926 CTTAAG C^TTAAG
BsaBI 2513 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2961 GCATTC GAATGC(1/-1)
BssSI 2106 CACGAG CACGAG(-5/-1)
BstBI 1132 TTCGAA TT^CGAA
BstEII 2710 GGTTACC G^GT[ACGT]ACC
BstPI 2710 GGTTACC G^GT[ACGT]ACC
DraIII 1308 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 5284 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2710 GGTTACC G^GT[ACGT]ACC
EcoRV 3042 GATATC GAT^ATC
EcoT22I 627 ATGCAT ATGCA^T
FspI 4249 TGCGCA TGC^GCA
MamI 2513 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3615 CAATTG C^AATTG
MluNI 5115 TGGCCA TGG^CCA
MscI 5115 TGGCCA TGG^CCA
MunI 3615 CAATTG C^AATTG
NsiI 627 ATGCAT ATGCA^T
NspV 1132 TTCGAA TT^CGAA
PflFI 4141 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 426 GTTTAAAC GTTT^AAAC
PshBI 3761 ATTAAT AT^TAAT
Psp1406I 3700 AACGTT AA^CGTT
SacI 5528 GAGCTC GAGCT^C
SfuI 1132 TTCGAA TT^CGAA
SmaI 5422 CCCGGG CCC^GGG
Tth111I 4141 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3664 TCTAGA T^CTAGA
XmaCI 5422 CCCGGG C^CCGGG
XmaI 5422 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 973 GTATAC GT^[AC][GT]AC
2977 GTATAC
AcyI 101 GGCGCC G[GA]^CG[CT]C
177 GGCGCC
AhdI 3677 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5438 GACCTGCGGTC
Aor51HI 3268 AGCGCT AGC^GCT
5343 AGCGCT
AspEI 3677 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5438 GACCTGCGGTC
BbeI 101 GGCGCC GGCGC^C
177 GGCGCC
BlpI 3706 GCTCAGC GC^T[ACGT]AGC
4973 GCTCAGC
Bpu1102I 3706 GCTCAGC GC^T[ACGT]AGC
4973 GCTCAGC
BsaHI 101 GGCGCC G[GA]^CG[CT]C
177 GGCGCC
BsaI 1772 GAGACC GGTCTC(1/5)
2435 GAGACC
BspDI 1866 ATCGAT AT^CGAT
3066 ATCGAT
BsrBI 146 GAGCGG CCGCTC(-3/-3)
4175 GAGCGG
BsrDI 216 GCAATG GCAATG(2/0)
4760 GCAATG
BssHII 136 GCGCGC G^CGCGC
138 GCGCGC
Bst1107I 973 GTATAC GTA^TAC
2977 GTATAC
BstZ17I 973 GTATAC GTA^TAC
2977 GTATAC
CelII 3706 GCTCAGC GC^T[ACGT]AGC
4973 GCTCAGC
ClaI 1866 ATCGAT AT^CGAT
3066 ATCGAT
DrdI 953 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5861 GACGGGAAGGTC
Eam1105I 3677 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5438 GACCTGCGGTC
Eco47III 3268 AGCGCT AGC^GCT
5343 AGCGCT
EcoRI 1727 GAATTC G^AATTC
5351 GAATTC
HgaI 133 GACGC GACGC(5/10)
2849 GACGC
Hin1I 101 GGCGCC G[GA]^CG[CT]C
177 GGCGCC
KasI 101 GGCGCC G^GCGCC
177 GGCGCC
KspI 173 CCGCGG CCGC^GG
5408 CCGCGG
NarI 101 GGCGCC GG^CGCC
177 GGCGCC
NcoI 329 CCATGG C^CATGG
5658 CCATGG
SacII 173 CCGCGG CCGC^GG
5408 CCGCGG
ScaI 460 AGTACT AGT^ACT
5505 AGTACT
SfoI 101 GGCGCC GGC^GCC
177 GGCGCC
SphI 4210 GCATGC GCATG^C
4455 GCATGC
SspI 3147 AATATT AAT^ATT
4354 AATATT
XcmI 3368 CCAGCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4335 CCAGCCGCATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI