##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
AvrII BamHI BbeI BciVI BclI
BfrI BglII BlnI BsaBI BsaHI
BsiWI BsmBI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
Eco47III EcoRI FbaI FseI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2126 GGTACC G^GTACC
AcsI 1780 GAATTT [GA]^AATT[CT]
AhdI 2893 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 197 GTGCAC G^TGCAC
ApaLI 197 GTGCAC G^TGCAC
ApoI 1780 GAATTT [GA]^AATT[CT]
Asp718I 2126 GGTACC G^GTACC
AspEI 2893 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1896 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 202 CACGTG CAC^GTG
BcgI 26 CGAAAGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 202 CACGTG [CT]AC^GT[GA]
BsiEI 1076 CGGCCG CG[GA][CT]^CG
Bsp1407I 3161 TGTACA T^GTACA
BspHI 328 TCATGA T^CATGA
BsrDI 4093 CATTGC GCAATG(2/0)
BsrGI 3161 TGTACA T^GTACA
DraI 3496 TTTAAA TTT^AAA
EagI 1076 CGGCCG C^GGCCG
Eam1105I 2893 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1076 CGGCCG C^GGCCG
Eco52I 1076 CGGCCG C^GGCCG
EcoNI 3447 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1831 GATATC GAT^ATC
EcoT22I 3732 ATGCAT ATGCA^T
KpnI 2126 GGTACC GGTAC^C
NaeI 3350 GCCGGC GCC^GGC
NcoI 277 CCATGG C^CATGG
NgoMIV 3350 GCCGGC G^CCGGC
NsiI 3732 ATGCAT ATGCA^T
PflFI 1896 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 202 CACGTG CAC^GTG
PmlI 202 CACGTG CAC^GTG
RcaI 328 TCATGA T^CATGA
SexAI 3744 ACCTGGT A^CC[AT]GGT
SmaI 1186 CCCGGG CCC^GGG
SphI 3390 GCATGC GCATG^C
SspBI 3161 TGTACA T^GTACA
SspI 2225 AATATT AAT^ATT
Tth111I 1896 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2414 CCACCTGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1186 CCCGGG C^CCGGG
XmaI 1186 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 617 GTCTAC GT^[AC][GT]AC
3965 GTCTAC
AccIII 3331 TCCGGA T^CCGGA
3413 TCCGGA
AlwNI 165 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2623 CAGTGTCTG
ApaI 572 GGGCCC GGGCC^C
1081 GGGCCC
AviII 562 TGCGCA TGC^GCA
1064 TGCGCA
BalI 3007 TGGCCA TGG^CCA
3025 TGGCCA
BbsI 3724 GAAGAC GAAGAC(2/6)
1902 GTCTTC
BbvCI 3801 CCTCAGC CCTCAGC(-5/-2)
4032 GCTGAGG
BpuAI 3724 GAAGAC GAAGAC(2/6)
1902 GTCTTC
BseAI 3331 TCCGGA T^CCGGA
3413 TCCGGA
BsmI 1501 GAATGC GAATGC(1/-1)
4097 GCATTC
BspEI 3331 TCCGGA T^CCGGA
3413 TCCGGA
BspLU11I 1467 ACATGT A^CATGT
3583 ACATGT
BssSI 678 CACGAG CACGAG(-5/-1)
1588 CACGAG
BstXI 3636 CCAAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3882 CCAGCACTTTGG
BstYI 2256 GGATCT [GA]^GATC[CT]
2869 GGATCT
DsaI 277 CCATGG C^C[GA][CT]GG
2903 CCGTGG
EcoT14I 277 CCATGG C^C[AT][AT]GG
3320 CCAAGG
FspI 562 TGCGCA TGC^GCA
1064 TGCGCA
HgaI 142 GCGTC GACGC(5/10)
258 GCGTC
MflI 2256 GGATCT [GA]^GATC[CT]
2869 GGATCT
MluNI 3007 TGGCCA TGG^CCA
3025 TGGCCA
MroI 3331 TCCGGA T^CCGGA
3413 TCCGGA
MscI 3007 TGGCCA TGG^CCA
3025 TGGCCA
PflMI 2717 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2999 CCAGCATTTGG
PstI 162 CTGCAG CTGCA^G
1775 CTGCAG
StyI 277 CCATGG C^C[AT][AT]GG
3320 CCAAGG
Van91I 2717 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2999 CCAGCATTTGG
XhoII 2256 GGATCT [GA]^GATC[CT]
2869 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AocI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I