##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AclI AflII AgeI AhdI AocI
Aor51HI AscI AspEI AvrII BamHI
BbvCI BclI BfrI BglI BglII
BlnI BsaBI BsmI BspDI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI Eam1105I Eco47III Eco81I EcoNI
FbaI FseI HpaI KspI MamI
MluI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI ScaI
SfiI SfuI SgrAI SnaBI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 2202 GACGTC GACGT^C
AccI 6505 GTAGAC GT^[AC][GT]AC
AseI 4855 ATTAAT AT^TAAT
AsnI 4855 ATTAAT AT^TAAT
AspI 4742 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2230 TGCGCA TGC^GCA
BbrPI 1692 CACGTG CAC^GTG
BcgI 1733 CGACAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiWI 1567 CGTACG C^GTACG
BstEII 1337 GGTCACC G^GT[ACGT]ACC
BstPI 1337 GGTCACC G^GT[ACGT]ACC
EcoO65I 1337 GGTCACC G^GT[ACGT]ACC
EcoRV 5749 GATATC GAT^ATC
EcoT22I 4808 ATGCAT ATGCA^T
FspI 2230 TGCGCA TGC^GCA
MfeI 5445 CAATTG C^AATTG
MunI 5445 CAATTG C^AATTG
NheI 5555 GCTAGC G^CTAGC
NsiI 4808 ATGCAT ATGCA^T
PflFI 4742 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1692 CACGTG CAC^GTG
PmlI 1692 CACGTG CAC^GTG
PshBI 4855 ATTAAT AT^TAAT
SexAI 1283 ACCTGGT A^CC[AT]GGT
SpeI 4328 ACTAGT A^CTAGT
SphI 599 GCATGC GCATG^C
SwaI 6349 ATTTAAAT ATTT^AAAT
Tth111I 4742 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4381 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1300 GGTACC G^GTACC
5782 GGTACC
AccIII 1631 TCCGGA T^CCGGA
2995 TCCGGA
AflIII 974 ACATGT A^C[GA][CT]GT
3726 ACATGT
Asp718I 1300 GGTACC G^GTACC
5782 GGTACC
BciVI 2108 GTATCC GTATCC(6/5)
5519 GTATCC
BsaAI 1413 TACGTG [CT]AC^GT[GA]
1692 CACGTG
BseAI 1631 TCCGGA T^CCGGA
2995 TCCGGA
Bsp1407I 1225 TGTACA T^GTACA
3333 TGTACA
BspEI 1631 TCCGGA T^CCGGA
2995 TCCGGA
BspLU11I 974 ACATGT A^CATGT
3726 ACATGT
BsrBI 2149 CCGCTC CCGCTC(-3/-3)
5565 CCGCTC
BsrGI 1225 TGTACA T^GTACA
3333 TGTACA
BssSI 915 CACGAG CACGAG(-5/-1)
2484 CACGAG
DraIII 2625 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2849 CACAGAGTG
HincII 2433 GTCAAC GT[CT]^[GA]AC
3730 GTCAAC
HindII 2433 GTCAAC GT[CT]^[GA]AC
3730 GTCAAC
KpnI 1300 GGTACC GGTAC^C
5782 GGTACC
MroI 1631 TCCGGA T^CCGGA
2995 TCCGGA
NaeI 175 GCCGGC GCC^GGC
1507 GCCGGC
NgoMIV 175 GCCGGC G^CCGGC
1507 GCCGGC
SapI 486 GAAGAGC GCTCTTC(1/4)
634 GAAGAGC
SbfI 1253 CCTGCAGG CCTGCA^GG
4965 CCTGCAGG
SmaI 196 CCCGGG CCC^GGG
625 CCCGGG
Sse8387I 1253 CCTGCAGG CCTGCA^GG
4965 CCTGCAGG
SspBI 1225 TGTACA T^GTACA
3333 TGTACA
StuI 5503 AGGCCT AGG^CCT
5673 AGGCCT
XcmI 442 CCAAGCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2903 CCACGCCCAGTTTGG
XmaCI 196 CCCGGG C^CCGGG
625 CCCGGG
XmaI 196 CCCGGG C^CCGGG
625 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI