##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI Asp718I BbrPI BseAI BsiWI
BsmBI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EcoO65I EcoRV FseI KpnI
KspI MfeI MluI MroI MunI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AflII 1105 CTTAAG C^TTAAG
AseI 6980 ATTAAT AT^TAAT
AsnI 6980 ATTAAT AT^TAAT
AvrII 4498 CCTAGG C^CTAGG
BamHI 4514 GGATCC G^GATCC
BcgI 1516 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1105 CTTAAG C^TTAAG
BlnI 4498 CCTAGG C^CTAGG
BsaBI 2159 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1339 ACCGGA [AT]^CCGG[AT]
BspLU11I 414 ACATGT A^CATGT
BsrBI 2689 GAGCGG CCGCTC(-3/-3)
DraI 4532 TTTAAA TTT^AAA
DraIII 4754 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
HindIII 3052 AAGCTT A^AGCTT
MamI 2159 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5075 GCCGGC GCC^GGC
NgoMIV 5075 GCCGGC G^CCGGC
PshAI 3445 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6980 ATTAAT AT^TAAT
SalI 4706 GTCGAC G^TCGAC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1841 GTCTAC GT^[AC][GT]AC
4706 GTCGAC
Alw44I 2982 GTGCAC G^TGCAC
4719 GTGCAC
ApaLI 2982 GTGCAC G^TGCAC
4719 GTGCAC
AviII 372 TGCGCA TGC^GCA
2666 TGCGCA
BbeI 2852 GGCGCC GGCGC^C
6726 GGCGCC
BbvCI 1088 GCTGAGG CCTCAGC(-5/-2)
5162 GCTGAGG
BciVI 3349 GGATAC GTATCC(6/5)
5500 GGATAC
BclI 3603 TGATCA T^GATCA
5674 TGATCA
BsaAI 2822 TACGTG [CT]AC^GT[GA]
4007 TACGTG
BsiEI 184 CGGCCG CG[GA][CT]^CG
2610 CGGCCG
BssSI 1653 CACGAG CACGAG(-5/-1)
3736 CACGAG
DrdI 3575 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6359 GACTTTCCTGTC
EagI 184 CGGCCG C^GGCCG
2610 CGGCCG
EclXI 184 CGGCCG C^GGCCG
2610 CGGCCG
Eco52I 184 CGGCCG C^GGCCG
2610 CGGCCG
EcoRI 3317 GAATTC G^AATTC
6230 GAATTC
FbaI 3603 TGATCA T^GATCA
5674 TGATCA
FspI 372 TGCGCA TGC^GCA
2666 TGCGCA
HpaI 4430 GTTAAC GTT^AAC
6441 GTTAAC
KasI 2852 GGCGCC G^GCGCC
6726 GGCGCC
NarI 2852 GGCGCC GG^CGCC
6726 GGCGCC
PaeR7I 1210 CTCGAG C^TCGAG
1368 CTCGAG
SbfI 1523 CCTGCAGG CCTGCA^GG
5029 CCTGCAGG
SexAI 4755 ACCTGGT A^CC[AT]GGT
6495 ACCAGGT
SfiI 2263 GGCCCCTACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3205 GGCCGTCGAGGCC
SfoI 2852 GGCGCC GGC^GCC
6726 GGCGCC
SmaI 2211 CCCGGG CCC^GGG
3954 CCCGGG
Sse8387I 1523 CCTGCAGG CCTGCA^GG
5029 CCTGCAGG
XcmI 3069 CCAAGTTAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3336 CCAGGACTAGCATGG
XhoI 1210 CTCGAG C^TCGAG
1368 CTCGAG
XmaCI 2211 CCCGGG C^CCGGG
3954 CCCGGG
XmaI 2211 CCCGGG C^CCGGG
3954 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflFI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XhoII XmnI