##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI AscI Asp718I AviII BbrPI
BfrI BglII BsiWI BspDI BspLU11I
BsrBI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eco47III EcoRI
EcoRV FseI FspI HpaI KpnI
KspI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SapI
SbfI SfiI SfuI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 5473 ACGTGT A^C[GA][CT]GT
AhdI 279 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5274 GTGCAC G^TGCAC
ApaLI 5274 GTGCAC G^TGCAC
AseI 5597 ATTAAT AT^TAAT
AsnI 5597 ATTAAT AT^TAAT
AspEI 279 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1809 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2282 CCTAGG C^CTAGG
BamHI 1111 GGATCC G^GATCC
BbeI 568 GGCGCC GGCGC^C
BciVI 3519 GGATAC GTATCC(6/5)
BglI 62 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2282 CCTAGG C^CTAGG
BsaAI 501 TACGTA [CT]AC^GT[GA]
BsaI 5521 GGTCTC GGTCTC(1/5)
BstAPI 2558 GCACATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 279 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4081 CCTTTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3590 ATGCAT ATGCA^T
HindIII 1973 AAGCTT A^AGCTT
KasI 568 GGCGCC G^GCGCC
NarI 568 GGCGCC GG^CGCC
NsiI 3590 ATGCAT ATGCA^T
PflFI 1809 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 252 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5597 ATTAAT AT^TAAT
SalI 4412 GTCGAC G^TCGAC
ScaI 4962 AGTACT AGT^ACT
SfoI 568 GGCGCC GGC^GCC
SgrAI 564 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 4362 CTCAAG C^T[CT][GA]AG
SnaBI 501 TACGTA TAC^GTA
StuI 3726 AGGCCT AGG^CCT
Tth111I 1809 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1431 GTCTAC GT^[AC][GT]AC
4412 GTCGAC
BbvCI 2344 CCTCAGC CCTCAGC(-5/-2)
4194 GCTGAGG
BcgI 3761 CGATTCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3781 CGATTCACCTGC
BclI 2720 TGATCA T^GATCA
3862 TGATCA
BsaBI 3857 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4845 GATGCTCATC
BsmBI 258 CGTCTC CGTCTC(1/5)
4128 CGTCTC
BsmI 1633 GCATTC GAATGC(1/-1)
4592 GCATTC
BspHI 1117 TCATGA T^CATGA
5187 TCATGA
BssSI 1746 CTCGTG CACGAG(-5/-1)
4512 CTCGTG
BstEII 4090 GGTTACC G^GT[ACGT]ACC
5298 GGTTACC
BstPI 4090 GGTTACC G^GT[ACGT]ACC
5298 GGTTACC
BstYI 1111 GGATCC [GA]^GATC[CT]
4547 GGATCT
DraI 5515 TTTAAA TTT^AAA
5553 TTTAAA
EagI 118 CGGCCG C^GGCCG
152 CGGCCG
EclXI 118 CGGCCG C^GGCCG
152 CGGCCG
Eco52I 118 CGGCCG C^GGCCG
152 CGGCCG
EcoO65I 4090 GGTTACC G^GT[ACGT]ACC
5298 GGTTACC
FbaI 2720 TGATCA T^GATCA
3862 TGATCA
HincII 1083 GTCAAC GT[CT]^[GA]AC
4412 GTCGAC
HindII 1083 GTCAAC GT[CT]^[GA]AC
4412 GTCGAC
MamI 3857 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4845 GATGCTCATC
MflI 1111 GGATCC [GA]^GATC[CT]
4547 GGATCT
PvuII 741 CAGCTG CAG^CTG
4660 CAGCTG
RcaI 1117 TCATGA T^CATGA
5187 TCATGA
SexAI 1399 ACCTGGT A^CC[AT]GGT
5178 ACCTGGT
XhoII 1111 GGATCC [GA]^GATC[CT]
4547 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspEI BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XmaCI XmaI XmnI