##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BalI BbrPI BcgI
BfrI BlnI BsaAI BsaBI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII BstEII BstPI BstUI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI FspI
KpnI KspI MamI MluI MluNI
MroI MscI MvnI NaeI NdeI
NgoMIV NotI NruI NsiI PacI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SgrAI SnaBI
Sse8387I StuI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 2490 GACGTC GACGT^C
AgeI 2166 ACCGGT A^CCGGT
AhdI 2891 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1307 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2891 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 208 GGCGCC G^G[CT][GA]CC
BbeI 208 GGCGCC GGCGC^C
BsmBI 2492 CGTCTC CGTCTC(1/5)
BspHI 4385 TCATGA T^CATGA
BspLU11I 4864 ACATGT A^CATGT
BspMI 4467 ACCTGC ACCTGC(4/8)
BsrFI 2166 ACCGGT [GA]^CCGG[CT]
BssSI 1266 CTCGTG CACGAG(-5/-1)
Bst1107I 6001 GTATAC GTA^TAC
BstAPI 4948 GCACTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 6001 GTATAC GTA^TAC
Cfr10I 2166 ACCGGT [GA]^CCGG[CT]
DraIII 4212 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2891 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 662 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1603 GAATTC G^AATTC
HgaI 5019 GACGC GACGC(5/10)
HpaI 4694 GTTAAC GTT^AAC
KasI 208 GGCGCC G^GCGCC
NarI 208 GGCGCC GG^CGCC
NheI 2561 GCTAGC G^CTAGC
PinAI 2166 ACCGGT A^CCGGT
RcaI 4385 TCATGA T^CATGA
ScaI 6112 AGTACT AGT^ACT
SfiI 665 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 208 GGCGCC GGC^GCC
SphI 5375 GCATGC GCATG^C
SspI 4814 AATATT AAT^ATT
XmnI 1307 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 5142 GTCTAC GT^[AC][GT]AC
6001 GTATAC
AcyI 208 GGCGCC G[GA]^CG[CT]C
2490 GACGTC
AflIII 4864 ACATGT A^C[GA][CT]GT
5599 ACGTGT
ApaI 1160 GGGCCC GGGCC^C
3877 GGGCCC
AspI 100 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3242 GACCTAGTC
BamHI 923 GGATCC G^GATCC
2435 GGATCC
BbvCI 2080 CCTCAGC CCTCAGC(-5/-2)
2000 GCTGAGG
BciVI 2627 GGATAC GTATCC(6/5)
931 GTATCC
BglII 320 AGATCT A^GATCT
1663 AGATCT
BsaHI 208 GGCGCC G[GA]^CG[CT]C
2490 GACGTC
BsaWI 2124 ACCGGA [AT]^CCGG[AT]
2166 ACCGGT
BseRI 2236 CTCCTC GAGGAG(10/8)
669 GAGGAG
BsmFI 3333 GTCCC GGGAC(10/14)
4131 GTCCC
BstBI 957 TTCGAA TT^CGAA
3192 TTCGAA
EcoRV 3023 GATATC GAT^ATC
3186 GATATC
FauI 630 CCCGC CCCGC(4/6)
522 GCGGG
Hin1I 208 GGCGCC G[GA]^CG[CT]C
2490 GACGTC
MfeI 2871 CAATTG C^AATTG
3043 CAATTG
MunI 2871 CAATTG C^AATTG
3043 CAATTG
NspV 957 TTCGAA TT^CGAA
3192 TTCGAA
PaeR7I 778 CTCGAG C^TCGAG
3694 CTCGAG
PflFI 100 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3242 GACCTAGTC
PpuMI 4030 AGGACCC [GA]G^G[AT]CC[CT]
5703 AGGTCCT
PstI 1092 CTGCAG CTGCA^G
2018 CTGCAG
SfuI 957 TTCGAA TT^CGAA
3192 TTCGAA
SmaI 251 CCCGGG CCC^GGG
1157 CCCGGG
SpeI 5839 ACTAGT A^CTAGT
5978 ACTAGT
Tth111I 100 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3242 GACCTAGTC
XhoI 778 CTCGAG C^TCGAG
3694 CTCGAG
XmaCI 251 CCCGGG C^CCGGG
1157 CCCGGG
XmaI 251 CCCGGG C^CCGGG
1157 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmI BsoBI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoII