##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AgeI AhdI Alw44I ApaI
ApaLI AscI AspEI BalI BamHI
BbrPI BsaAI BsaBI BsmBI BssHII
BstBI BstEII BstPI BstXI DrdI
Eam1105I EcoO65I EcoT22I FseI HpaI
MamI MfeI MluI MluNI MscI
MunI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI SalI SfiI
SfuI SgrAI SmaI SnaBI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2395 GGTACC G^GTACC
AccIII 1057 TCCGGA T^CCGGA
AclI 3066 AACGTT AA^CGTT
AcyI 683 GGCGCC G[GA]^CG[CT]C
AflIII 4863 ACATGT A^C[GA][CT]GT
Aor51HI 1872 AGCGCT AGC^GCT
Asp700I 4804 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2395 GGTACC G^GTACC
AspI 3496 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 80 TGCGCA TGC^GCA
BbeI 683 GGCGCC GGCGC^C
BglII 2792 AGATCT A^GATCT
BlpI 2140 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2140 GCTGAGC GC^T[ACGT]AGC
BsaHI 683 GGCGCC G[GA]^CG[CT]C
BsaI 216 GAGACC GGTCTC(1/5)
BseAI 1057 TCCGGA T^CCGGA
BsgI 1884 GTGCAG GTGCAG(16/14)
BsiWI 715 CGTACG C^GTACG
BspDI 50 ATCGAT AT^CGAT
BspEI 1057 TCCGGA T^CCGGA
BspLU11I 4863 ACATGT A^CATGT
Bst1107I 33 GTATAC GTA^TAC
BstZ17I 33 GTATAC GTA^TAC
CelII 2140 GCTGAGC GC^T[ACGT]AGC
ClaI 50 ATCGAT AT^CGAT
CpoI 1059 CGGACCG CG^G[AT]CCG
DraIII 1972 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2460 CGGCCG C^GGCCG
EclXI 2460 CGGCCG C^GGCCG
Eco47III 1872 AGCGCT AGC^GCT
Eco52I 2460 CGGCCG C^GGCCG
EcoRI 3892 GAATTC G^AATTC
EcoRV 2884 GATATC GAT^ATC
FspI 80 TGCGCA TGC^GCA
Hin1I 683 GGCGCC G[GA]^CG[CT]C
KasI 683 GGCGCC G^GCGCC
KpnI 2395 GGTACC GGTAC^C
MroI 1057 TCCGGA T^CCGGA
NarI 683 GGCGCC GG^CGCC
NdeI 1930 CATATG CA^TATG
PflFI 3496 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3066 AACGTT AA^CGTT
RsrII 1059 CGGACCG CG^G[AT]CCG
SbfI 872 CCTGCAGG CCTGCA^GG
SexAI 417 ACCTGGT A^CC[AT]GGT
SfoI 683 GGCGCC GGC^GCC
SpeI 5043 ACTAGT A^CTAGT
Sse8387I 872 CCTGCAGG CCTGCA^GG
Tth111I 3496 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4804 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AflII 1316 CTTAAG C^TTAAG
3979 CTTAAG
AlwI 466 GGATC GGATC(4/5)
2738 GGATC
AocI 366 CCTCAGG CC^T[ACGT]AGG
2209 CCTCAGG
AvaI 2062 CCCGAG C^[CT]CG[GA]G
2650 CTCGGG
AvrII 2566 CCTAGG C^CTAGG
3248 CCTAGG
BbsI 525 GTCTTC GAAGAC(2/6)
755 GTCTTC
BbvCI 254 CCTCAGC CCTCAGC(-5/-2)
348 CCTCAGC
BcgI 5640 CGATGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
982 GCAACGTGATCG
BciVI 2722 GGATAC GTATCC(6/5)
183 GTATCC
BfrI 1316 CTTAAG C^TTAAG
3979 CTTAAG
BlnI 2566 CCTAGG C^CTAGG
3248 CCTAGG
BpuAI 525 GTCTTC GAAGAC(2/6)
755 GTCTTC
BsmAI 216 GAGAC GTCTC(1/5)
5289 GAGAC
BsoBI 2062 CCCGAG C^[CT]CG[GA]G
2650 CTCGGG
BspMI 4971 ACCTGC ACCTGC(4/8)
1432 GCAGGT
BsrFI 248 GCCGGC [GA]^CCGG[CT]
611 GCCGGC
BssSI 2426 CTCGTG CACGAG(-5/-1)
2594 CTCGTG
BstAPI 83 GCAAACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
768 GCAGCCGCTGC
BstYI 2738 GGATCT [GA]^GATC[CT]
2792 AGATCT
Bsu36I 366 CCTCAGG CC^T[ACGT]AGG
2209 CCTCAGG
Cfr10I 248 GCCGGC [GA]^CCGG[CT]
611 GCCGGC
EarI 492 CTCTTC CTCTTC(1/4)
3204 GAAGAG
Eco81I 366 CCTCAGG CC^T[ACGT]AGG
2209 CCTCAGG
HgaI 1186 GACGC GACGC(5/10)
390 GCGTC
HincII 477 GTCAAC GT[CT]^[GA]AC
516 GTCAAC
HindII 477 GTCAAC GT[CT]^[GA]AC
516 GTCAAC
Ksp632I 492 CTCTTC CTCTTC(1/4)
3204 GAAGAG
KspI 376 CCGCGG CCGC^GG
709 CCGCGG
MflI 2738 GGATCT [GA]^GATC[CT]
2792 AGATCT
NaeI 248 GCCGGC GCC^GGC
611 GCCGGC
NcoI 1289 CCATGG C^CATGG
1632 CCATGG
NgoMIV 248 GCCGGC G^CCGGC
611 GCCGGC
NheI 3739 GCTAGC G^CTAGC
3771 GCTAGC
PflMI 856 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1030 CCAAGTCCTGG
PleI 174 GAGTC GAGTC(4/5)
2937 GAGTC
PvuII 809 CAGCTG CAG^CTG
2941 CAGCTG
SacI 3446 GAGCTC GAGCT^C
3517 GAGCTC
SacII 376 CCGCGG CCGC^GG
709 CCGCGG
SapI 3204 GAAGAGC GCTCTTC(1/4)
491 GCTCTTC
ScaI 2167 AGTACT AGT^ACT
3294 AGTACT
StuI 3733 AGGCCT AGG^CCT
3765 AGGCCT
Van91I 856 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1030 CCAAGTCCTGG
XcmI 1030 CCAAGTCCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4154 CCAGCCCAGGTGTGG
XhoII 2738 GGATCT [GA]^GATC[CT]
2792 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwNI ApoI AseI AsnI
AspHI AvaII BanI BanII BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BsrBI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PshBI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI