##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspI AviII AvrII BamHI
BbeI BcgI BciVI BclI BlnI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BsrFI BssHII
BssSI BstAPI BstXI Cfr10I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoRI EcoRV FbaI FseI FspI
HaeII HgaI Hin1I HpaI KasI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI PflFI
PinAI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I Tth111I
XcmI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 534 GGTACC G^GTACC
AccI 5914 GTATAC GT^[AC][GT]AC
AclI 4887 AACGTT AA^CGTT
Asp718I 534 GGTACC G^GTACC
BbvCI 1095 CCTCAGC CCTCAGC(-5/-2)
BglI 191 GCCTGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4136 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4136 GCTTAGC GC^T[ACGT]AGC
BsrBI 255 GAGCGG CCGCTC(-3/-3)
Bst1107I 5914 GTATAC GTA^TAC
BstBI 5762 TTCGAA TT^CGAA
BstEII 4457 GGTCACC G^GT[ACGT]ACC
BstPI 4457 GGTCACC G^GT[ACGT]ACC
BstZ17I 5914 GTATAC GTA^TAC
CelII 4136 GCTTAGC GC^T[ACGT]AGC
DsaI 3231 CCATGG C^C[GA][CT]GG
EcoO65I 4457 GGTCACC G^GT[ACGT]ACC
KpnI 534 GGTACC GGTAC^C
NcoI 3231 CCATGG C^CATGG
NspV 5762 TTCGAA TT^CGAA
PaeR7I 1869 CTCGAG C^TCGAG
PmeI 6050 GTTTAAAC GTTT^AAAC
PshAI 1102 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4887 AACGTT AA^CGTT
SfuI 5762 TTCGAA TT^CGAA
SmaI 114 CCCGGG CCC^GGG
SwaI 2170 ATTTAAAT ATTT^AAAT
XbaI 5813 TCTAGA T^CTAGA
XhoI 1869 CTCGAG C^TCGAG
XmaCI 114 CCCGGG C^CCGGG
XmaI 114 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 4683 CTTAAG C^TTAAG
5890 CTTAAG
AspHI 495 GTGCTC G[AT]GC[AT]^C
2586 GTGCTC
BanII 86 GGGCTC G[GA]GC[CT]^C
318 GAGCCC
BbrPI 137 CACGTG CAC^GTG
480 CACGTG
BfrI 4683 CTTAAG C^TTAAG
5890 CTTAAG
BpmI 232 CTCCAG CTGGAG(16/14)
5133 CTCCAG
BsaBI 802 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4663 GATATTTATC
BseRI 3724 CTCCTC GAGGAG(10/8)
624 GAGGAG
BsiEI 37 CGGCCG CG[GA][CT]^CG
258 CGGCCG
BsiHKAI 495 GTGCTC G[AT]GC[AT]^C
2586 GTGCTC
BsmI 4607 GAATGC GAATGC(1/-1)
1115 GCATTC
Bsp1407I 2444 TGTACA T^GTACA
3254 TGTACA
BspHI 4879 TCATGA T^CATGA
5068 TCATGA
BsrDI 1726 GCAATG GCAATG(2/0)
3287 GCAATG
BsrGI 2444 TGTACA T^GTACA
3254 TGTACA
EagI 37 CGGCCG C^GGCCG
258 CGGCCG
EclXI 37 CGGCCG C^GGCCG
258 CGGCCG
Eco52I 37 CGGCCG C^GGCCG
258 CGGCCG
HincII 1649 GTCAAC GT[CT]^[GA]AC
3279 GTTGAC
HindII 1649 GTCAAC GT[CT]^[GA]AC
3279 GTTGAC
HindIII 1268 AAGCTT A^AGCTT
5766 AAGCTT
MamI 802 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4663 GATATTTATC
PacI 2820 TTAATTAA TTAAT^TAA
3827 TTAATTAA
PflMI 206 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1227 CCAGAAAGTGG
PmaCI 137 CACGTG CAC^GTG
480 CACGTG
PmlI 137 CACGTG CAC^GTG
480 CACGTG
PvuII 561 CAGCTG CAG^CTG
5032 CAGCTG
RcaI 4879 TCATGA T^CATGA
5068 TCATGA
SapI 4070 GCTCTTC GCTCTTC(1/4)
5646 GCTCTTC
SspBI 2444 TGTACA T^GTACA
3254 TGTACA
Van91I 206 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1227 CCAGAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AvaI AvaII
BalI BanI BbsI BbvI BcnI
BfaI BglII BmyI BpuAI BsaAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PshBI PstI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI