##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccII AccIII AgeI Aor51HI ApaI
AscI AviII AvrII BamHI BbeI
BcgI BglI BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I FseI FspI HincII HindII
HpaI KasI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI NspV PinAI PpuMI
PshAI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1372 GACGTC GACGT^C
AclI 3331 AACGTT AA^CGTT
AcyI 1372 GACGTC G[GA]^CG[CT]C
AhdI 774 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4124 CCTGAGG CC^T[ACGT]AGG
AspEI 774 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1515 CTCGAG C^[CT]CG[GA]G
BbrPI 6017 CACGTG CAC^GTG
BclI 5852 TGATCA T^GATCA
BcnI 1533 CCCGG CC^[GC]GG
BlpI 2182 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2182 GCTTAGC GC^T[ACGT]AGC
BsaAI 6017 CACGTG [CT]AC^GT[GA]
BsaHI 1372 GACGTC G[GA]^CG[CT]C
BsmBI 4935 CGTCTC CGTCTC(1/5)
BsoBI 1515 CTCGAG C^[CT]CG[GA]G
BsrBI 1335 CCGCTC CCGCTC(-3/-3)
Bst1107I 4000 GTATAC GTA^TAC
BstAPI 5222 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4000 GTATAC GTA^TAC
Bsu36I 4124 CCTGAGG CC^T[ACGT]AGG
CelII 2182 GCTTAGC GC^T[ACGT]AGC
DraII 5224 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 5059 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 774 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4124 CCTGAGG CC^T[ACGT]AGG
EcoO109I 5224 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 5412 GAATTC G^AATTC
EcoRV 2503 GATATC GAT^ATC
EcoT22I 6341 ATGCAT ATGCA^T
FauI 1421 CCCGC CCCGC(4/6)
FbaI 5852 TGATCA T^GATCA
HaeII 4407 AGCGCC [GA]GCGC^[CT]
HapII 1534 CCGG C^CGG
Hin1I 1372 GACGTC G[GA]^CG[CT]C
HpaII 1534 CCGG C^CGG
MspI 1534 CCGG C^CGG
NciI 1533 CCCGG CC^[GC]GG
NcoI 3266 CCATGG C^CATGG
NheI 3883 GCTAGC G^CTAGC
NsiI 6341 ATGCAT ATGCA^T
PacI 4782 TTAATTAA TTAAT^TAA
PaeR7I 1515 CTCGAG C^TCGAG
PmaCI 6017 CACGTG CAC^GTG
PmeI 1080 GTTTAAAC GTTT^AAAC
PmlI 6017 CACGTG CAC^GTG
Psp1406I 3331 AACGTT AA^CGTT
SapI 95 GCTCTTC GCTCTTC(1/4)
SpeI 333 ACTAGT A^CTAGT
XhoI 1515 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2903 GTCTAC GT^[AC][GT]AC
4000 GTATAC
AflII 2661 CTTAAG C^TTAAG
6183 CTTAAG
Alw44I 1027 GTGCAC G^TGCAC
4708 GTGCAC
ApaLI 1027 GTGCAC G^TGCAC
4708 GTGCAC
Asp700I 5548 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6069 GAATGTCTTC
AspI 3698 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5467 GACTTGGTC
AvaII 65 GGTCC G^G[AT]CC
2205 GGACC
BbvCI 4403 CCTCAGC CCTCAGC(-5/-2)
2345 GCTGAGG
BfrI 2661 CTTAAG C^TTAAG
6183 CTTAAG
BglII 1685 AGATCT A^GATCT
5202 AGATCT
BsaI 193 GAGACC GGTCTC(1/5)
3033 GAGACC
BspLU11I 5808 ACATGT A^CATGT
6377 ACATGT
DrdI 1099 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1351 GACAAGAAGGTC
HgaI 378 GACGC GACGC(5/10)
4934 GCGTC
HindIII 688 AAGCTT A^AGCTT
3992 AAGCTT
PflFI 3698 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5467 GACTTGGTC
PflMI 1032 CCACGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1398 CCACTATCTGG
ScaI 739 AGTACT AGT^ACT
793 AGTACT
SphI 4599 GCATGC GCATG^C
6058 GCATGC
StuI 989 AGGCCT AGG^CCT
5124 AGGCCT
Tth111I 3698 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5467 GACTTGGTC
Van91I 1032 CCACGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1398 CCACTATCTGG
XbaI 2472 TCTAGA T^CTAGA
4956 TCTAGA
XcmI 221 CCAGCAGCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6009 CCAGAACCCACGTGG
XmnI 5548 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6069 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp718I AspHI BalI BanI
BanII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MunI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII