##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AclI Alw44I AocI
ApaI ApaLI AscI AspHI AviII
AvrII BalI BbeI BbvCI BglI
BglII BlnI BlpI Bpu1102I BseAI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BspHI BsrBI BssHII BssSI
BstAPI BstEII BstPI Bsu36I CelII
ClaI CpoI DrdI EaeI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
FseI FspI HindIII KasI KspI
MluI MluNI MroI MscI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PmeI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI Sse8387I StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1990 GGTACC G^GTACC
AcyI 680 GACGCC G[GA]^CG[CT]C
AgeI 1445 ACCGGT A^CCGGT
Aor51HI 887 AGCGCT AGC^GCT
Asp700I 4440 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1990 GGTACC G^GTACC
AspI 285 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 719 GGATCC G^GATCC
BbrPI 1682 CACGTG CAC^GTG
BcgI 4012 GCATTTTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4396 GGATAC GTATCC(6/5)
BpmI 847 CTGGAG CTGGAG(16/14)
BsaAI 1682 CACGTG [CT]AC^GT[GA]
BsaHI 680 GACGCC G[GA]^CG[CT]C
BsaI 1632 GAGACC GGTCTC(1/5)
BspLU11I 3001 ACATGT A^CATGT
BspMI 342 GCAGGT ACCTGC(4/8)
BsrFI 1445 ACCGGT [GA]^CCGG[CT]
BstBI 437 TTCGAA TT^CGAA
Cfr10I 1445 ACCGGT [GA]^CCGG[CT]
DraIII 709 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 887 AGCGCT AGC^GCT
EcoRI 476 GAATTC G^AATTC
EcoRV 1772 GATATC GAT^ATC
EcoT22I 1167 ATGCAT ATGCA^T
HaeII 887 AGCGCT [GA]GCGC^[CT]
Hin1I 680 GACGCC G[GA]^CG[CT]C
KpnI 1990 GGTACC GGTAC^C
MfeI 619 CAATTG C^AATTG
MunI 619 CAATTG C^AATTG
NdeI 3785 CATATG CA^TATG
NheI 806 GCTAGC G^CTAGC
NsiI 1167 ATGCAT ATGCA^T
NspV 437 TTCGAA TT^CGAA
PflFI 285 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1204 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1445 ACCGGT A^CCGGT
PmaCI 1682 CACGTG CAC^GTG
PmlI 1682 CACGTG CAC^GTG
PstI 1421 CTGCAG CTGCA^G
SapI 1356 GAAGAGC GCTCTTC(1/4)
SfuI 437 TTCGAA TT^CGAA
SmaI 79 CCCGGG CCC^GGG
Tth111I 285 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1204 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4159 TCTAGA T^CTAGA
XcmI 218 CCATTATAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 79 CCCGGG C^CCGGG
XmaI 79 CCCGGG C^CCGGG
XmnI 4440 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 3259 CTTAAG C^TTAAG
4531 CTTAAG
AflIII 1683 ACGTGT A^C[GA][CT]GT
3001 ACATGT
AlwNI 1424 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1981 CAGGATCTG
AvaI 72 CCCGAG C^[CT]CG[GA]G
79 CCCGGG
BanI 1990 GGTACC G^G[CT][GA]CC
2040 GGTGCC
BanII 36 GGGCTC G[GA]GC[CT]^C
75 GAGCCC
BfrI 3259 CTTAAG C^TTAAG
4531 CTTAAG
BsaBI 2500 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2514 GATTGGGATC
BsaWI 362 ACCGGA [AT]^CCGG[AT]
1445 ACCGGT
BsgI 547 GTGCAG GTGCAG(16/14)
1531 GTGCAG
BsmAI 1392 GAGAC GTCTC(1/5)
1632 GAGAC
BsmI 1808 GAATGC GAATGC(1/-1)
4196 GAATGC
BsoBI 72 CCCGAG C^[CT]CG[GA]G
79 CCCGGG
Bsp1407I 2468 TGTACA T^GTACA
2482 TGTACA
BsrGI 2468 TGTACA T^GTACA
2482 TGTACA
BstXI 227 CCATGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
486 CCATTGCACTGG
DraII 469 GGGACCT [GA]G^G[ACGT]CC[CT]
4308 AGGTCCT
EcoO109I 469 GGGACCT [GA]G^G[ACGT]CC[CT]
4308 AGGTCCT
HgaI 680 GACGC GACGC(5/10)
21 GCGTC
HincII 756 GTTAAC GT[CT]^[GA]AC
3871 GTTAAC
HindII 756 GTTAAC GT[CT]^[GA]AC
3871 GTTAAC
HpaI 756 GTTAAC GTT^AAC
3871 GTTAAC
MamI 2500 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2514 GATTGGGATC
PpuMI 469 GGGACCT [GA]G^G[AT]CC[CT]
4308 AGGTCCT
SmlI 3259 CTTAAG C^T[CT][GA]AG
4531 CTTAAG
SphI 106 GCATGC GCATG^C
2104 GCATGC
SspBI 2468 TGTACA T^GTACA
2482 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AhdI AluI AlwI ApoI AseI
AsnI AspEI AvaII BbsI BbvI
BclI BcnI BfaI BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI Bst1107I
BstF5I BstNI BstUI BstYI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI Eam1105I EarI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SpeI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII