##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AccIII AclI AgeI Aor51HI
AscI AseI AsnI BamHI BciVI
BclI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
EagI EarI EclXI Eco47III Eco52I
EcoRV FbaI FseI HincII HindII
HpaI Ksp632I KspI MamI MluI
MroI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SfiI SfuI SgrAI SnaBI
SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 1588 GACGTC GACGT^C
Acc65I 556 GGTACC G^GTACC
AflII 3704 CTTAAG C^TTAAG
AhdI 1828 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1969 GTGCAC G^TGCAC
ApaLI 1969 GTGCAC G^TGCAC
Asp700I 1140 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 556 GGTACC G^GTACC
AspEI 1828 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3111 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1658 TGCGCA TGC^GCA
AvrII 2693 CCTAGG C^CTAGG
BbeI 1636 GGCGCC GGCGC^C
BbrPI 153 CACGTG CAC^GTG
BcgI 148 CGATGCACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3704 CTTAAG C^TTAAG
BglII 3567 AGATCT A^GATCT
BlnI 2693 CCTAGG C^CTAGG
BlpI 739 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 739 GCTGAGC GC^T[ACGT]AGC
BsaI 3775 GAGACC GGTCTC(1/5)
BsmBI 1288 GAGACG CGTCTC(1/5)
BsmI 3276 GCATTC GAATGC(1/-1)
BsrDI 427 GCAATG GCAATG(2/0)
BssHII 6 GCGCGC G^CGCGC
CelII 739 GCTGAGC GC^T[ACGT]AGC
DraIII 1558 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 856 GACAAGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1828 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3342 ATGCAT ATGCA^T
FspI 1658 TGCGCA TGC^GCA
KasI 1636 GGCGCC G^GCGCC
KpnI 556 GGTACC GGTAC^C
MfeI 3246 CAATTG C^AATTG
MslI 792 CACATTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3246 CAATTG C^AATTG
NaeI 2080 GCCGGC GCC^GGC
NarI 1636 GGCGCC GG^CGCC
NcoI 191 CCATGG C^CATGG
NdeI 2075 CATATG CA^TATG
NgoMIV 2080 GCCGGC G^CCGGC
NheI 1476 GCTAGC G^CTAGC
NsiI 3342 ATGCAT ATGCA^T
PaeR7I 1330 CTCGAG C^TCGAG
PflFI 3111 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 153 CACGTG CAC^GTG
PmlI 153 CACGTG CAC^GTG
SacI 376 GAGCTC GAGCT^C
SbfI 1017 CCTGCAGG CCTGCA^GG
ScaI 1196 AGTACT AGT^ACT
SfoI 1636 GGCGCC GGC^GCC
SmaI 3044 CCCGGG CCC^GGG
SphI 650 GCATGC GCATG^C
Sse8387I 1017 CCTGCAGG CCTGCA^GG
SspI 3989 AATATT AAT^ATT
Tth111I 3111 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1330 CTCGAG C^TCGAG
XmaCI 3044 CCCGGG C^CCGGG
XmaI 3044 CCCGGG C^CCGGG
XmnI 1140 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1588 GACGTC G[GA]^CG[CT]C
1636 GGCGCC
AocI 2903 CCTGAGG CC^T[ACGT]AGG
4344 CCTAAGG
ApaI 1037 GGGCCC GGGCC^C
1237 GGGCCC
BbsI 853 GAAGAC GAAGAC(2/6)
2415 GTCTTC
BglI 679 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3646 GCCGCAGTGGC
BpuAI 853 GAAGAC GAAGAC(2/6)
2415 GTCTTC
BsaAI 153 CACGTG [CT]AC^GT[GA]
3206 CACGTA
BsaHI 1588 GACGTC G[GA]^CG[CT]C
1636 GGCGCC
Bsp1407I 3160 TGTACA T^GTACA
4387 TGTACA
BspLU11I 3163 ACATGT A^CATGT
4043 ACATGT
BsrFI 294 GCCGGT [GA]^CCGG[CT]
2080 GCCGGC
BsrGI 3160 TGTACA T^GTACA
4387 TGTACA
BstAPI 726 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1705 GCATGGACTGC
BstYI 2366 AGATCC [GA]^GATC[CT]
3567 AGATCT
Bsu36I 2903 CCTGAGG CC^T[ACGT]AGG
4344 CCTAAGG
Cfr10I 294 GCCGGT [GA]^CCGG[CT]
2080 GCCGGC
DsaI 191 CCATGG C^C[GA][CT]GG
2385 CCACGG
Eco81I 2903 CCTGAGG CC^T[ACGT]AGG
4344 CCTAAGG
EcoNI 705 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1269 CCTGCAAAAGG
EcoRI 2134 GAATTC G^AATTC
2914 GAATTC
HgaI 1290 GACGC GACGC(5/10)
927 GCGTC
Hin1I 1588 GACGTC G[GA]^CG[CT]C
1636 GGCGCC
HindIII 2527 AAGCTT A^AGCTT
4338 AAGCTT
MflI 2366 AGATCC [GA]^GATC[CT]
3567 AGATCT
MspA1I 58 CAGCGG C[AC]G^C[GT]G
642 CCGCTG
SspBI 3160 TGTACA T^GTACA
4387 TGTACA
XhoII 2366 AGATCC [GA]^GATC[CT]
3567 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI