##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccII AccIII AcyI AflIII
AgeI AhdI ApaI AscI AseI
AsnI AspEI AspI AvrII BamHI
BbeI BbrPI BbvCI BglI BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII BssSI BstBI BstUI
ClaI CpoI DsaI EagI Eam1105I
EclXI Eco52I EcoRI FseI Hin1I
HindIII KasI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NcoI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacII SalI SapI SfiI
SfoI SfuI SgrAI SnaBI StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 4225 GGTACC G^GTACC
AclI 2134 AACGTT AA^CGTT
AflII 1641 CTTAAG C^TTAAG
Alw44I 1962 GTGCAC G^TGCAC
AocI 3670 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1477 AGCGCT AGC^GCT
ApaLI 1962 GTGCAC G^TGCAC
Asp700I 1167 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4225 GGTACC G^GTACC
AvaI 285 CCCGGG C^[CT]CG[GA]G
BanI 4225 GGTACC G^G[CT][GA]CC
BciVI 3311 GTATCC GTATCC(6/5)
BclI 2012 TGATCA T^GATCA
BfrI 1641 CTTAAG C^TTAAG
BsmBI 417 GAGACG CGTCTC(1/5)
BsoBI 285 CCCGGG C^[CT]CG[GA]G
BspHI 144 TCATGA T^CATGA
BstAPI 1350 GCAAATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 219 GGTCACC G^GT[ACGT]ACC
BstPI 219 GGTCACC G^GT[ACGT]ACC
BstXI 4211 CCATCTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3670 CCTGAGG CC^T[ACGT]AGG
DraII 1462 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 742 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4662 GACACTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2794 CTCTTC CTCTTC(1/4)
Eco47III 1477 AGCGCT AGC^GCT
Eco81I 3670 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1462 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 219 GGTCACC G^GT[ACGT]ACC
EcoRV 326 GATATC GAT^ATC
EcoT14I 3389 CCTTGG C^C[AT][AT]GG
EcoT22I 4303 ATGCAT ATGCA^T
FauI 3366 CCCGC CCCGC(4/6)
FbaI 2012 TGATCA T^GATCA
HgaI 595 GCGTC GACGC(5/10)
HincII 272 GTTAAC GT[CT]^[GA]AC
HindII 272 GTTAAC GT[CT]^[GA]AC
HpaI 272 GTTAAC GTT^AAC
KpnI 4225 GGTACC GGTAC^C
Ksp632I 2794 CTCTTC CTCTTC(1/4)
MspA1I 1604 CAGCTG C[AC]G^C[GT]G
NaeI 3661 GCCGGC GCC^GGC
NdeI 3096 CATATG CA^TATG
NgoMIV 3661 GCCGGC G^CCGGC
NsiI 4303 ATGCAT ATGCA^T
NspI 3486 GCATGC [GA]CATG^[CT]
Psp1406I 2134 AACGTT AA^CGTT
PvuII 1604 CAGCTG CAG^CTG
RcaI 144 TCATGA T^CATGA
SbfI 3102 CCTGCAGG CCTGCA^GG
SexAI 743 ACCAGGT A^CC[AT]GGT
SmaI 285 CCCGGG CCC^GGG
SphI 3486 GCATGC GCATG^C
Sse8387I 3102 CCTGCAGG CCTGCA^GG
SspI 3152 AATATT AAT^ATT
StyI 3389 CCTTGG C^C[AT][AT]GG
XmaCI 285 CCCGGG C^CCGGG
XmaI 285 CCCGGG C^CCGGG
XmnI 1167 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 986 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3509 CAGGAGCTG
AviII 3473 TGCGCA TGC^GCA
3483 TGCGCA
BalI 940 TGGCCA TGG^CCA
3076 TGGCCA
BanII 794 GAGCTC G[GA]GC[CT]^C
1952 GAGCTC
BcgI 866 CGAACCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1340 CGATGATTTTGC
BglII 1259 AGATCT A^GATCT
4795 AGATCT
BlpI 1990 GCTTAGC GC^T[ACGT]AGC
4647 GCTCAGC
Bpu1102I 1990 GCTTAGC GC^T[ACGT]AGC
4647 GCTCAGC
BsaI 1008 GAGACC GGTCTC(1/5)
1389 GAGACC
BspMI 869 ACCTGC ACCTGC(4/8)
2050 GCAGGT
BsrDI 1577 CATTGC GCAATG(2/0)
1783 GCAATG
BsrFI 2020 GCCGGT [GA]^CCGG[CT]
3661 GCCGGC
CelII 1990 GCTTAGC GC^T[ACGT]AGC
4647 GCTCAGC
Cfr10I 2020 GCCGGT [GA]^CCGG[CT]
3661 GCCGGC
EcoNI 1058 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3670 CCTGAGGGAGG
FspI 3473 TGCGCA TGC^GCA
3483 TGCGCA
HaeII 1477 AGCGCT [GA]GCGC^[CT]
1522 GGCGCT
MluNI 940 TGGCCA TGG^CCA
3076 TGGCCA
MscI 940 TGGCCA TGG^CCA
3076 TGGCCA
NheI 4096 GCTAGC G^CTAGC
4529 GCTAGC
PflMI 741 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3377 CCAGAGTGTGG
SacI 794 GAGCTC GAGCT^C
1952 GAGCTC
ScaI 172 AGTACT AGT^ACT
1681 AGTACT
Van91I 741 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3377 CCAGAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstYI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspBI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII