##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AviII BbrPI
BsiWI BspLU11I Bst1107I BstEII BstPI
BstZ17I DraIII EcoO65I EcoRI FspI
HpaI KpnI MfeI MluI MunI
NdeI NotI NruI PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI SalI SapI SgrAI SnaBI
SspI SwaI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1939 GTCTAC GT^[AC][GT]AC
AccIII 2310 TCCGGA T^CCGGA
AflIII 398 ACGTGT A^C[GA][CT]GT
AhdI 574 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2717 GTGCAC G^TGCAC
ApaLI 2717 GTGCAC G^TGCAC
Asp700I 4949 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 574 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 491 TGGCCA TGG^CCA
BamHI 258 GGATCC G^GATCC
BcgI 2430 GCAAGCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5102 TGATCA T^GATCA
BglII 3634 AGATCT A^GATCT
BseAI 2310 TCCGGA T^CCGGA
BsmBI 2842 CGTCTC CGTCTC(1/5)
Bsp1407I 3222 TGTACA T^GTACA
BspDI 386 ATCGAT AT^CGAT
BspEI 2310 TCCGGA T^CCGGA
BsrBI 4477 GAGCGG CCGCTC(-3/-3)
BsrDI 405 CATTGC GCAATG(2/0)
BsrGI 3222 TGTACA T^GTACA
BssSI 1288 CACGAG CACGAG(-5/-1)
BstAPI 4649 GCAAATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 671 TTCGAA TT^CGAA
ClaI 386 ATCGAT AT^CGAT
CpoI 2195 CGGACCG CG^G[AT]CCG
Eam1105I 574 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2534 GATATC GAT^ATC
FbaI 5102 TGATCA T^GATCA
FseI 31 GGCCGGCC GGCCGG^CC
HincII 238 GTTGAC GT[CT]^[GA]AC
HindII 238 GTTGAC GT[CT]^[GA]AC
MluNI 491 TGGCCA TGG^CCA
MroI 2310 TCCGGA T^CCGGA
MscI 491 TGGCCA TGG^CCA
NcoI 3740 CCATGG C^CATGG
NheI 4645 GCTAGC G^CTAGC
NspV 671 TTCGAA TT^CGAA
PshAI 373 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2195 CGGACCG CG^G[AT]CCG
ScaI 1176 AGTACT AGT^ACT
SexAI 1046 ACCTGGT A^CC[AT]GGT
SfiI 3932 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 671 TTCGAA TT^CGAA
SmaI 60 CCCGGG CCC^GGG
SpeI 3926 ACTAGT A^CTAGT
SphI 2867 GCATGC GCATG^C
SspBI 3222 TGTACA T^GTACA
XbaI 1605 TCTAGA T^CTAGA
XmaCI 60 CCCGGG C^CCGGG
XmaI 60 CCCGGG C^CCGGG
XmnI 4949 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 2412 CTTAAG C^TTAAG
4981 CTTAAG
AlwNI 1060 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2006 CAGTGCCTG
Aor51HI 1093 AGCGCT AGC^GCT
1361 AGCGCT
AspI 1723 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2837 GACCTCGTC
AvrII 2820 CCTAGG C^CTAGG
4104 CCTAGG
BciVI 1495 GTATCC GTATCC(6/5)
4242 GTATCC
BfrI 2412 CTTAAG C^TTAAG
4981 CTTAAG
BglI 2794 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3933 GCCTCCTGGGC
BlnI 2820 CCTAGG C^CTAGG
4104 CCTAGG
BsaAI 397 TACGTG [CT]AC^GT[GA]
2471 CACGTA
BsaBI 1611 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2233 GATGAGGATC
BsaWI 590 ACCGGA [AT]^CCGG[AT]
2310 TCCGGA
BsiEI 30 CGGCCG CG[GA][CT]^CG
73 CGGCCG
BspHI 1385 TCATGA T^CATGA
1733 TCATGA
DrdI 1249 GACGGGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1678 GACATCTCTGTC
EagI 30 CGGCCG C^GGCCG
73 CGGCCG
EclXI 30 CGGCCG C^GGCCG
73 CGGCCG
Eco47III 1093 AGCGCT AGC^GCT
1361 AGCGCT
Eco52I 30 CGGCCG C^GGCCG
73 CGGCCG
EcoT22I 3258 ATGCAT ATGCA^T
4192 ATGCAT
HindIII 2432 AAGCTT A^AGCTT
3657 AAGCTT
MamI 1611 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2233 GATGAGGATC
NaeI 32 GCCGGC GCC^GGC
151 GCCGGC
NgoMIV 32 GCCGGC G^CCGGC
151 GCCGGC
NsiI 3258 ATGCAT ATGCA^T
4192 ATGCAT
PflFI 1723 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2837 GACCTCGTC
RcaI 1385 TCATGA T^CATGA
1733 TCATGA
SacI 2137 GAGCTC GAGCT^C
4904 GAGCTC
Tth111I 1723 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2837 GACCTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfoI SmlI
Sse8387I StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII