##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AviII AvrII BamHI BanI
BbeI BbrPI BfrI BglII BlnI
BsaAI BsiWI BspDI BsrBI BssHII
BstBI BstEII BstPI Bsu36I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HaeII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 779 GACGTC GACGT^C
AccII 113 CGCG CG^CG
AccIII 1910 TCCGGA T^CCGGA
AhdI 78 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 78 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 1678 TGATCA T^GATCA
BlpI 1501 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1501 GCTGAGC GC^T[ACGT]AGC
BseAI 1910 TCCGGA T^CCGGA
BsiEI 84 CGGTCG CG[GA][CT]^CG
Bsp1407I 4674 TGTACA T^GTACA
BspEI 1910 TCCGGA T^CCGGA
BspMI 660 ACCTGC ACCTGC(4/8)
BsrFI 1368 ACCGGC [GA]^CCGG[CT]
BsrGI 4674 TGTACA T^GTACA
BssSI 1577 CTCGTG CACGAG(-5/-1)
Bst1107I 250 GTATAC GTA^TAC
BstUI 113 CGCG CG^CG
BstZ17I 250 GTATAC GTA^TAC
CelII 1501 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1368 ACCGGC [GA]^CCGG[CT]
DraIII 3871 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1057 CCATGG C^C[GA][CT]GG
Eam1105I 78 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1334 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1785 ATGCAT ATGCA^T
FauI 4564 CCCGC CCCGC(4/6)
FbaI 1678 TGATCA T^GATCA
HgaI 1013 GACGC GACGC(5/10)
HindIII 2041 AAGCTT A^AGCTT
MroI 1910 TCCGGA T^CCGGA
MvnI 113 CGCG CG^CG
NcoI 1057 CCATGG C^CATGG
NdeI 3516 CATATG CA^TATG
NsiI 1785 ATGCAT ATGCA^T
SspBI 4674 TGTACA T^GTACA
XbaI 3635 TCTAGA T^CTAGA
XcmI 90 CCATGATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 250 GTATAC GT^[AC][GT]AC
2523 GTAGAC
AcyI 779 GACGTC G[GA]^CG[CT]C
1013 GACGCC
AspI 2420 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4025 GACAGGGTC
AvaI 840 CCCGGG C^[CT]CG[GA]G
4405 CCCGGG
BalI 4211 TGGCCA TGG^CCA
4526 TGGCCA
BanII 1177 GAGCCC G[GA]GC[CT]^C
4408 GGGCTC
BcgI 4107 CGATTATTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4417 CGATTCTCTTGC
BglI 89 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3431 GCCCAGAAGGC
BsaBI 889 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3375 GATTTTCATC
BsaHI 779 GACGTC G[GA]^CG[CT]C
1013 GACGCC
BsaWI 82 TCCGGT [AT]^CCGG[AT]
1910 TCCGGA
BsmBI 781 CGTCTC CGTCTC(1/5)
243 GAGACG
BsmI 5005 GAATGC GAATGC(1/-1)
3879 GCATTC
BsoBI 840 CCCGGG C^[CT]CG[GA]G
4405 CCCGGG
BspHI 2862 TCATGA T^CATGA
4554 TCATGA
BstAPI 4083 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5000 GCAATGAATGC
CfoI 1066 GCGC GCG^C
4998 GCGC
EaeI 4211 TGGCCA [CT]^GGCC[GA]
4526 TGGCCA
EcoRI 3590 GAATTC G^AATTC
5032 GAATTC
HhaI 1066 GCGC GCG^C
4998 GCGC
Hin1I 779 GACGTC G[GA]^CG[CT]C
1013 GACGCC
HinP1I 1066 GCGC G^CGC
4998 GCGC
MamI 889 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3375 GATTTTCATC
MluNI 4211 TGGCCA TGG^CCA
4526 TGGCCA
MscI 4211 TGGCCA TGG^CCA
4526 TGGCCA
MspA1I 2049 CAGCTG C[AC]G^C[GT]G
2444 CAGCTG
PflFI 2420 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4025 GACAGGGTC
PvuII 2049 CAGCTG CAG^CTG
2444 CAGCTG
RcaI 2862 TCATGA T^CATGA
4554 TCATGA
SmaI 840 CCCGGG CCC^GGG
4405 CCCGGG
SphI 4148 GCATGC GCATG^C
4460 GCATGC
Tth111I 2420 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4025 GACAGGGTC
XmaCI 840 CCCGGG C^CCGGG
4405 CCCGGG
XmaI 840 CCCGGG C^CCGGG
4405 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI