##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspI
AvaI AviII BbeI BbrPI BcgI
BciVI BglI BlpI BpmI Bpu1102I
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI BspEI BsrBI
BsrFI BssHII BstBI BstEII BstPI
BstUI Bsu36I CelII CfoI Cfr10I
CpoI DraIII DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AlwNI 1680 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 4209 GTGCTC G[AT]GC[AT]^C
AvrII 4863 CCTAGG C^CTAGG
BanII 1649 GGGCTC G[GA]GC[CT]^C
BclI 2076 TGATCA T^GATCA
BcnI 1856 CCCGG CC^[GC]GG
BglII 3792 AGATCT A^GATCT
BlnI 4863 CCTAGG C^CTAGG
BsaBI 204 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4513 GGTCTC GGTCTC(1/5)
BsiHKAI 4209 GTGCTC G[AT]GC[AT]^C
BspDI 4521 ATCGAT AT^CGAT
BsrDI 3317 CATTGC GCAATG(2/0)
BssSI 5044 CTCGTG CACGAG(-5/-1)
Bst1107I 3470 GTATAC GTA^TAC
BstAPI 1869 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1466 CCAGATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3470 GTATAC GTA^TAC
ClaI 4521 ATCGAT AT^CGAT
DraII 4821 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 4043 GACTCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 4821 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 328 GAATTC G^AATTC
EcoRV 1746 GATATC GAT^ATC
EcoT14I 4863 CCTAGG C^C[AT][AT]GG
FauI 455 GCGGG CCCGC(4/6)
FbaI 2076 TGATCA T^GATCA
HapII 1857 CCGG C^CGG
HincII 2866 GTTAAC GT[CT]^[GA]AC
HindII 2866 GTTAAC GT[CT]^[GA]AC
HpaI 2866 GTTAAC GTT^AAC
HpaII 1857 CCGG C^CGG
MamI 204 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 717 CAATTG C^AATTG
MspI 1857 CCGG C^CGG
MunI 717 CAATTG C^AATTG
NciI 1856 CCCGG CC^[GC]GG
PshAI 4903 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SpeI 4203 ACTAGT A^CTAGT
SphI 2851 GCATGC GCATG^C
StuI 3973 AGGCCT AGG^CCT
StyI 4863 CCTAGG C^C[AT][AT]GG
XbaI 3592 TCTAGA T^CTAGA
XcmI 1711 CCATAATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1290 GTAGAC GT^[AC][GT]AC
3470 GTATAC
AflII 1352 CTTAAG C^TTAAG
3407 CTTAAG
AhdI 4084 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4706 GACCTGCTGTC
Asp700I 841 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2589 GAATCATTTC
AspEI 4084 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4706 GACCTGCTGTC
BamHI 1419 GGATCC G^GATCC
1955 GGATCC
BanI 38 GGCACC G^G[CT][GA]CC
239 GGCACC
BfrI 1352 CTTAAG C^TTAAG
3407 CTTAAG
BmyI 1649 GGGCTC G[AGT]GC[ACT]^C
4209 GTGCTC
BsgI 5 CTGCAC GTGCAG(16/14)
3550 GTGCAG
Bsp1286I 1649 GGGCTC G[AGT]GC[ACT]^C
4209 GTGCTC
Eam1105I 4084 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4706 GACCTGCTGTC
EcoT22I 61 ATGCAT ATGCA^T
2558 ATGCAT
NsiI 61 ATGCAT ATGCA^T
2558 ATGCAT
SapI 3041 GAAGAGC GCTCTTC(1/4)
3073 GCTCTTC
SnaBI 4370 TACGTA TAC^GTA
5156 TACGTA
XmnI 841 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2589 GAATCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BalI BbsI BbvCI BbvI BfaI
BpuAI BsaAI BsaJI BsiYI BslI
BsmAI BsmFI BsmI Bsp1407I BspHI
BspLU11I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI SwaI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII