##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AvrII BcgI
BfrI BglII BlnI BsaBI BsiWI
BspDI BspLU11I BsrDI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
EcoRV EcoT22I FseI HpaI KpnI
KspI MamI MfeI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI SacII SalI
SbfI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 313 TCCGGA T^CCGGA
AcsI 1368 GAATTC [GA]^AATT[CT]
ApoI 1368 GAATTC [GA]^AATT[CT]
AspI 4621 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1076 CACGTG CAC^GTG
BbvCI 2280 CCTCAGC CCTCAGC(-5/-2)
BciVI 716 GGATAC GTATCC(6/5)
BsaAI 1076 CACGTG [CT]AC^GT[GA]
BsaWI 313 TCCGGA [AT]^CCGG[AT]
BseAI 313 TCCGGA T^CCGGA
BsmI 1965 GAATGC GAATGC(1/-1)
Bsp1407I 2449 TGTACA T^GTACA
BspEI 313 TCCGGA T^CCGGA
BsrGI 2449 TGTACA T^GTACA
BstEII 3542 GGTGACC G^GT[ACGT]ACC
BstPI 3542 GGTGACC G^GT[ACGT]ACC
CpoI 651 CGGTCCG CG^G[AT]CCG
DrdI 3360 GACAGGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3542 GGTGACC G^GT[ACGT]ACC
EcoRI 1368 GAATTC G^AATTC
HincII 162 GTCAAC GT[CT]^[GA]AC
HindII 162 GTCAAC GT[CT]^[GA]AC
HindIII 4593 AAGCTT A^AGCTT
MluI 1921 ACGCGT A^CGCGT
MroI 313 TCCGGA T^CCGGA
PflFI 4621 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1076 CACGTG CAC^GTG
PmlI 1076 CACGTG CAC^GTG
RsrII 651 CGGTCCG CG^G[AT]CCG
SmlI 4590 CTCAAG C^T[CT][GA]AG
SspBI 2449 TGTACA T^GTACA
Tth111I 4621 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3342 CCAGCCGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 159 GACGTC GACGT^C
3924 GACGTC
AccI 2666 GTCTAC GT^[AC][GT]AC
3334 GTCTAC
AflIII 1921 ACGCGT A^C[GA][CT]GT
4615 ACGTGT
Alw44I 1915 GTGCAC G^TGCAC
4116 GTGCAC
ApaLI 1915 GTGCAC G^TGCAC
4116 GTGCAC
Asp700I 1412 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3911 GAAGTTTTTC
BamHI 811 GGATCC G^GATCC
3263 GGATCC
BbeI 620 GGCGCC GGCGC^C
2906 GGCGCC
BlpI 604 GCTCAGC GC^T[ACGT]AGC
1969 GCTGAGC
Bpu1102I 604 GCTCAGC GC^T[ACGT]AGC
1969 GCTGAGC
CelII 604 GCTCAGC GC^T[ACGT]AGC
1969 GCTGAGC
DraI 3714 TTTAAA TTT^AAA
4598 TTTAAA
DraIII 2742 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
3937 CACTCAGTG
KasI 620 GGCGCC G^GCGCC
2906 GGCGCC
NaeI 2333 GCCGGC GCC^GGC
2954 GCCGGC
NarI 620 GGCGCC GG^CGCC
2906 GGCGCC
NgoMIV 2333 GCCGGC G^CCGGC
2954 GCCGGC
PstI 2880 CTGCAG CTGCA^G
3723 CTGCAG
SapI 1032 GAAGAGC GCTCTTC(1/4)
4539 GCTCTTC
ScaI 532 AGTACT AGT^ACT
1216 AGTACT
SfoI 620 GGCGCC GGC^GCC
2906 GGCGCC
SmaI 6 CCCGGG CCC^GGG
3668 CCCGGG
StuI 2416 AGGCCT AGG^CCT
3845 AGGCCT
XbaI 3409 TCTAGA T^CTAGA
4692 TCTAGA
XmaCI 6 CCCGGG C^CCGGG
3668 CCCGGG
XmaI 6 CCCGGG C^CCGGG
3668 CCCGGG
XmnI 1412 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3911 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI Aor51HI
ApaI AspEI AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfiI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII