##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I BamHI BciVI BfrI
BseAI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraIII EagI EclXI Eco52I
EcoO65I FseI HpaI KpnI MluI
MroI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 2047 GACGTC GACGT^C
AccI 1113 GTAGAC GT^[AC][GT]AC
AhdI 321 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2031 AGCGCT AGC^GCT
AspEI 321 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1811 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 46 CTCGGG C^[CT]CG[GA]G
AviII 2346 TGCGCA TGC^GCA
AvrII 3127 CCTAGG C^CTAGG
BalI 4265 TGGCCA TGG^CCA
BbeI 68 GGCGCC GGCGC^C
BbrPI 841 CACGTG CAC^GTG
BcgI 2061 CGACTGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4267 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 856 AGATCT A^GATCT
BlnI 3127 CCTAGG C^CTAGG
BlpI 79 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 79 GCTGAGC GC^T[ACGT]AGC
BsaAI 841 CACGTG [CT]AC^GT[GA]
BsaBI 1093 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 939 GGTCTC GGTCTC(1/5)
BsiEI 40 CGGTCG CG[GA][CT]^CG
BsoBI 46 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2145 TGTACA T^GTACA
BsrGI 2145 TGTACA T^GTACA
BssSI 860 CTCGTG CACGAG(-5/-1)
CelII 79 GCTGAGC GC^T[ACGT]AGC
DraII 602 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 3772 GACACTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4265 TGGCCA [CT]^GGCC[GA]
Eam1105I 321 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2031 AGCGCT AGC^GCT
EcoO109I 602 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1244 GATATC GAT^ATC
EcoT22I 2747 ATGCAT ATGCA^T
FspI 2346 TGCGCA TGC^GCA
HgaI 1664 GACGC GACGC(5/10)
HincII 2161 GTTGAC GT[CT]^[GA]AC
HindII 2161 GTTGAC GT[CT]^[GA]AC
HindIII 383 AAGCTT A^AGCTT
KasI 68 GGCGCC G^GCGCC
KspI 72 CCGCGG CCGC^GG
MamI 1093 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3503 CAATTG C^AATTG
MluNI 4265 TGGCCA TGG^CCA
MscI 4265 TGGCCA TGG^CCA
MunI 3503 CAATTG C^AATTG
NarI 68 GGCGCC GG^CGCC
NdeI 3847 CATATG CA^TATG
NsiI 2747 ATGCAT ATGCA^T
PflFI 1811 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 841 CACGTG CAC^GTG
PmlI 841 CACGTG CAC^GTG
SacII 72 CCGCGG CCGC^GG
SfoI 68 GGCGCC GGC^GCC
SphI 4604 GCATGC GCATG^C
SspBI 2145 TGTACA T^GTACA
SspI 3856 AATATT AAT^ATT
Tth111I 1811 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4443 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 1959 CCTCAGG CC^T[ACGT]AGG
3479 CCTCAGG
BbvCI 4307 CCTCAGC CCTCAGC(-5/-2)
1853 GCTGAGG
BclI 573 TGATCA T^GATCA
1740 TGATCA
BsaWI 139 ACCGGA [AT]^CCGG[AT]
2542 TCCGGT
BsmI 580 GAATGC GAATGC(1/-1)
1218 GAATGC
BspHI 1105 TCATGA T^CATGA
3180 TCATGA
BstAPI 89 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4680 GCATGTGCTGC
BstXI 4152 CCAGGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4358 CCATTGGCCTGG
BstYI 856 AGATCT [GA]^GATC[CT]
3444 GGATCT
Bsu36I 1959 CCTCAGG CC^T[ACGT]AGG
3479 CCTCAGG
Eco81I 1959 CCTCAGG CC^T[ACGT]AGG
3479 CCTCAGG
EcoRI 836 GAATTC G^AATTC
4060 GAATTC
FbaI 573 TGATCA T^GATCA
1740 TGATCA
HaeII 68 GGCGCC [GA]GCGC^[CT]
2031 AGCGCT
MflI 856 AGATCT [GA]^GATC[CT]
3444 GGATCT
PflMI 919 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1939 CCATTCCATGG
RcaI 1105 TCATGA T^CATGA
3180 TCATGA
SacI 184 GAGCTC GAGCT^C
1792 GAGCTC
SapI 714 GAAGAGC GCTCTTC(1/4)
1693 GAAGAGC
SmlI 731 CTTGAG C^T[CT][GA]AG
1138 CTTGAG
SpeI 4195 ACTAGT A^CTAGT
4660 ACTAGT
Van91I 919 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1939 CCATTCCATGG
XhoII 856 AGATCT [GA]^GATC[CT]
3444 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmnI