##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I ApaLI AscI Asp718I
AviII BamHI BbrPI BbvCI BcgI
BfrI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI EclXI Eco52I EcoNI EcoRV
EcoT22I FseI FspI HgaI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacI SacII SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AhdI 1641 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1423 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 4580 AGCGCT AGC^GCT
AseI 3802 ATTAAT AT^TAAT
AsnI 3802 ATTAAT AT^TAAT
AspEI 1641 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2993 CCTAGG C^CTAGG
BalI 4116 TGGCCA TGG^CCA
BciVI 3312 GGATAC GTATCC(6/5)
BclI 722 TGATCA T^GATCA
BglII 1220 AGATCT A^GATCT
BlnI 2993 CCTAGG C^CTAGG
BseRI 4019 CTCCTC GAGGAG(10/8)
BsmBI 3930 GAGACG CGTCTC(1/5)
BspMI 4154 ACCTGC ACCTGC(4/8)
BstEII 3297 GGTAACC G^GT[ACGT]ACC
BstPI 3297 GGTAACC G^GT[ACGT]ACC
DraII 237 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 3897 CCATGG C^C[GA][CT]GG
Eam1105I 1641 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4580 AGCGCT AGC^GCT
EcoO109I 237 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3297 GGTAACC G^GT[ACGT]ACC
FbaI 722 TGATCA T^GATCA
HindIII 2360 AAGCTT A^AGCTT
MluNI 4116 TGGCCA TGG^CCA
MscI 4116 TGGCCA TGG^CCA
NcoI 3897 CCATGG C^CATGG
NdeI 4218 CATATG CA^TATG
PshAI 3014 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3802 ATTAAT AT^TAAT
PstI 150 CTGCAG CTGCA^G
PvuII 3624 CAGCTG CAG^CTG
SalI 1163 GTCGAC G^TCGAC
SapI 459 GCTCTTC GCTCTTC(1/4)
SfaNI 246 GCATC GCATC(5/9)
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 16 GGCGCC G[GA]^CG[CT]C
4054 GGCGCC
AflIII 1228 ACATGT A^C[GA][CT]GT
3070 ACATGT
AlwI 4149 GATCC GGATC(4/5)
2629 GGATC
ApaI 3454 GGGCCC GGGCC^C
3647 GGGCCC
AspHI 4321 GAGCAC G[AT]GC[AT]^C
4343 GAGCAC
AspI 2706 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3932 GACGGAGTC
BanI 16 GGCGCC G^G[CT][GA]CC
4054 GGCGCC
BbeI 16 GGCGCC GGCGC^C
4054 GGCGCC
BsaAI 2763 TACGTG [CT]AC^GT[GA]
4563 CACGTA
BsaHI 16 GGCGCC G[GA]^CG[CT]C
4054 GGCGCC
BsgI 221 GTGCAG GTGCAG(16/14)
3963 GTGCAG
BsiHKAI 4321 GAGCAC G[AT]GC[AT]^C
4343 GAGCAC
BsiYI 79 CCGGGCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
112 CCTCAGGCCGG
BslI 79 CCGGGCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
112 CCTCAGGCCGG
BsmFI 138 GGGAC GGGAC(10/14)
3477 GTCCC
BsmI 619 GAATGC GAATGC(1/-1)
2564 GAATGC
Bsp1407I 647 TGTACA T^GTACA
2648 TGTACA
BspLU11I 1228 ACATGT A^CATGT
3070 ACATGT
BsrGI 647 TGTACA T^GTACA
2648 TGTACA
BssSI 563 CTCGTG CACGAG(-5/-1)
3465 CTCGTG
BstAPI 3658 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3989 GCAAGCTCTGC
BstYI 1220 AGATCT [GA]^GATC[CT]
2629 GGATCT
FauI 241 CCCGC CCCGC(4/6)
4058 CCCGC
Hin1I 16 GGCGCC G[GA]^CG[CT]C
4054 GGCGCC
KasI 16 GGCGCC G^GCGCC
4054 GGCGCC
MflI 1220 AGATCT [GA]^GATC[CT]
2629 GGATCT
MspA1I 29 CCGCTG C[AC]G^C[GT]G
3624 CAGCTG
NarI 16 GGCGCC GG^CGCC
4054 GGCGCC
PflFI 2706 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3932 GACGGAGTC
SfoI 16 GGCGCC GGC^GCC
4054 GGCGCC
SspBI 647 TGTACA T^GTACA
2648 TGTACA
Tth111I 2706 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3932 GACGGAGTC
XbaI 789 TCTAGA T^CTAGA
2172 TCTAGA
XhoII 1220 AGATCT [GA]^GATC[CT]
2629 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AocI ApoI Asp700I AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BsmAI BsoBI Bsp1286I
BspHI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI EaeI EarI Eco81I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SexAI SfcI SmaI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmaCI XmaI XmnI