##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BbeI BbrPI BsaAI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV FseI FspI KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI Van91I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 731 GACGCC G[GA]^CG[CT]C
AflII 3421 CTTAAG C^TTAAG
AgeI 4961 ACCGGT A^CCGGT
AocI 4169 CCTCAGG CC^T[ACGT]AGG
BciVI 3038 GTATCC GTATCC(6/5)
BfrI 3421 CTTAAG C^TTAAG
BglII 5826 AGATCT A^GATCT
BsaBI 2665 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 731 GACGCC G[GA]^CG[CT]C
BsaI 5427 GGTCTC GGTCTC(1/5)
BsaWI 4961 ACCGGT [AT]^CCGG[AT]
BseRI 2275 GAGGAG GAGGAG(10/8)
BsiEI 1255 CGGTCG CG[GA][CT]^CG
BspMI 3404 ACCTGC ACCTGC(4/8)
BsrBI 100 CCGCTC CCGCTC(-3/-3)
Bsu36I 4169 CCTCAGG CC^T[ACGT]AGG
DraII 2967 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 3165 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3259 GACATGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4169 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2967 AGGCCCC [GA]G^G[ACGT]CC[CT]
Hin1I 731 GACGCC G[GA]^CG[CT]C
HindIII 1875 AAGCTT A^AGCTT
HpaI 5668 GTTAAC GTT^AAC
MamI 2665 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 2333 CTCGAG C^TCGAG
PinAI 4961 ACCGGT A^CCGGT
PstI 72 CTGCAG CTGCA^G
SacI 3719 GAGCTC GAGCT^C
SexAI 4466 ACCTGGT A^CC[AT]GGT
SmaI 278 CCCGGG CCC^GGG
SphI 2912 GCATGC GCATG^C
StuI 1123 AGGCCT AGG^CCT
SwaI 4298 ATTTAAAT ATTT^AAAT
XhoI 2333 CTCGAG C^TCGAG
XmaCI 278 CCCGGG C^CCGGG
XmaI 278 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1384 GTCTAC GT^[AC][GT]AC
2053 GTCTAC
AflIII 3260 ACATGT A^C[GA][CT]GT
3464 ACATGT
Aor51HI 18 AGCGCT AGC^GCT
3194 AGCGCT
AspHI 62 GAGCAC G[AT]GC[AT]^C
3719 GAGCTC
AspI 4689 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5740 GACATTGTC
BamHI 2359 GGATCC G^GATCC
4286 GGATCC
BbvCI 4046 CCTCAGC CCTCAGC(-5/-2)
4187 CCTCAGC
BclI 387 TGATCA T^GATCA
4143 TGATCA
BglI 77 GCCGGTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
238 GCCAGTCAGGC
BsiHKAI 62 GAGCAC G[AT]GC[AT]^C
3719 GAGCTC
BspHI 2839 TCATGA T^CATGA
4155 TCATGA
BspLU11I 3260 ACATGT A^CATGT
3464 ACATGT
Eco47III 18 AGCGCT AGC^GCT
3194 AGCGCT
EcoT22I 5052 ATGCAT ATGCA^T
5236 ATGCAT
FbaI 387 TGATCA T^GATCA
4143 TGATCA
HaeII 18 AGCGCT [GA]GCGC^[CT]
3194 AGCGCT
MfeI 530 CAATTG C^AATTG
3500 CAATTG
MunI 530 CAATTG C^AATTG
3500 CAATTG
NdeI 4415 CATATG CA^TATG
5086 CATATG
NsiI 5052 ATGCAT ATGCA^T
5236 ATGCAT
PflFI 4689 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5740 GACATTGTC
RcaI 2839 TCATGA T^CATGA
4155 TCATGA
ScaI 1274 AGTACT AGT^ACT
3234 AGTACT
Tsp45I 1538 GTGAC ^GT[GC]AC
2195 GTGAC
Tth111I 4689 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5740 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoNI
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmnI