##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AcsI AflII ApoI
AscI AseI AsnI Asp718I AviII
BclI BfrI BsaBI BsiWI BspDI
BspLU11I BsrDI Bst1107I BstBI BstZ17I
ClaI CpoI DraIII EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RsrII SalI SapI
SfiI SfuI SnaBI SpeI SphI
SspI SwaI TfiI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3765 GACGTC GACGT^C
AccIII 1549 TCCGGA T^CCGGA
AflIII 1759 ACGTGT A^C[GA][CT]GT
AgeI 2740 ACCGGT A^CCGGT
AhdI 549 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2558 CCTCAGG CC^T[ACGT]AGG
AspEI 549 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2247 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2292 GGATCC G^GATCC
BbrPI 2550 CACGTG CAC^GTG
BciVI 2991 GGATAC GTATCC(6/5)
BglII 3265 AGATCT A^GATCT
BseAI 1549 TCCGGA T^CCGGA
BspEI 1549 TCCGGA T^CCGGA
BstEII 3563 GGTCACC G^GT[ACGT]ACC
BstPI 3563 GGTCACC G^GT[ACGT]ACC
Bsu36I 2558 CCTCAGG CC^T[ACGT]AGG
DraI 4871 TTTAAA TTT^AAA
Eam1105I 549 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2558 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3563 GGTCACC G^GT[ACGT]ACC
HincII 2406 GTCAAC GT[CT]^[GA]AC
HindII 2406 GTCAAC GT[CT]^[GA]AC
MroI 1549 TCCGGA T^CCGGA
MseI 4872 TTAA T^TAA
NdeI 4854 CATATG CA^TATG
PflFI 2247 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2740 ACCGGT A^CCGGT
PmaCI 2550 CACGTG CAC^GTG
PmlI 2550 CACGTG CAC^GTG
PshAI 1374 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 3680 CCTGCAGG CCTGCA^GG
ScaI 3169 AGTACT AGT^ACT
SgrAI 268 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1149 CTCAAG C^T[CT][GA]AG
Sse8387I 3680 CCTGCAGG CCTGCA^GG
Tru9I 4872 TTAA T^TAA
Tth111I 2247 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1464 GTCTAC GT^[AC][GT]AC
1719 GTCTAC
Aor51HI 1837 AGCGCT AGC^GCT
2572 AGCGCT
Asp700I 335 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1757 GAACGTGTTC
AvrII 4364 CCTAGG C^CTAGG
4421 CCTAGG
BbvCI 2888 CCTCAGC CCTCAGC(-5/-2)
777 GCTGAGG
BlnI 4364 CCTAGG C^CTAGG
4421 CCTAGG
BsaAI 828 TACGTG [CT]AC^GT[GA]
2550 CACGTG
BsaWI 1549 TCCGGA [AT]^CCGG[AT]
2740 ACCGGT
BsmI 1394 GAATGC GAATGC(1/-1)
3155 GAATGC
BssSI 999 CACGAG CACGAG(-5/-1)
2595 CTCGTG
EarI 1257 CTCTTC CTCTTC(1/4)
1583 GAAGAG
Eco47III 1837 AGCGCT AGC^GCT
2572 AGCGCT
EcoNI 599 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4776 CCTCTCCCAGG
Ksp632I 1257 CTCTTC CTCTTC(1/4)
1583 GAAGAG
PflMI 613 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2447 CCAGCGGGTGG
SacI 392 GAGCTC GAGCT^C
2421 GAGCTC
Tsp509I 2036 AATT ^AATT
4726 AATT
Van91I 613 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2447 CCAGCGGGTGG
XmnI 335 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1757 GAACGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SacII Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SspBI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI