##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AgeI AocI
Aor51HI ApaI AscI AviII AvrII
BamHI BanII BbrPI BcgI BciVI
BlnI BsaI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI BstBI BstEII BstPI BstXI
Bsu36I ClaI DrdI Eco47III Eco81I
EcoO65I EcoRI FseI FspI HgaI
MluI MroI NdeI NheI NotI
NspV PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI SacI
SapI SbfI SexAI SfiI SfuI
SgrAI SpeI Sse8387I SspBI XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1325 GGTACC G^GTACC
AcyI 212 GGCGCC G[GA]^CG[CT]C
AflII 3958 CTTAAG C^TTAAG
Alw44I 4869 GTGCAC G^TGCAC
AlwI 893 GATCC GGATC(4/5)
ApaLI 4869 GTGCAC G^TGCAC
AseI 15 ATTAAT AT^TAAT
AsnI 15 ATTAAT AT^TAAT
Asp700I 3230 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1325 GGTACC G^GTACC
AspI 3986 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 436 TGGCCA TGG^CCA
BbeI 212 GGCGCC GGCGC^C
BbvCI 3551 GCTGAGG CCTCAGC(-5/-2)
BclI 3197 TGATCA T^GATCA
BfrI 3958 CTTAAG C^TTAAG
BlpI 242 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 242 GCTGAGC GC^T[ACGT]AGC
BsaBI 3198 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 212 GGCGCC G[GA]^CG[CT]C
BsgI 1947 CTGCAC GTGCAG(16/14)
BsmI 2370 GCATTC GAATGC(1/-1)
BspLU11I 2441 ACATGT A^CATGT
BsrBI 189 GAGCGG CCGCTC(-3/-3)
Bst1107I 2408 GTATAC GTA^TAC
BstZ17I 2408 GTATAC GTA^TAC
CelII 242 GCTGAGC GC^T[ACGT]AGC
CpoI 228 CGGTCCG CG^G[AT]CCG
DraIII 1045 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1240 GATATC GAT^ATC
EcoT22I 4340 ATGCAT ATGCA^T
FbaI 3197 TGATCA T^GATCA
HaeII 212 GGCGCC [GA]GCGC^[CT]
Hin1I 212 GGCGCC G[GA]^CG[CT]C
KasI 212 GGCGCC G^GCGCC
KpnI 1325 GGTACC GGTAC^C
MamI 3198 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5055 CAATTG C^AATTG
MluNI 436 TGGCCA TGG^CCA
MscI 436 TGGCCA TGG^CCA
MunI 5055 CAATTG C^AATTG
NarI 212 GGCGCC GG^CGCC
NcoI 439 CCATGG C^CATGG
NruI 112 TCGCGA TCG^CGA
NsiI 4340 ATGCAT ATGCA^T
PacI 3527 TTAATTAA TTAAT^TAA
PflFI 3986 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 15 ATTAAT AT^TAAT
PstI 2077 CTGCAG CTGCA^G
PvuII 1857 CAGCTG CAG^CTG
RsrII 228 CGGTCCG CG^G[AT]CCG
SalI 1440 GTCGAC G^TCGAC
SfoI 212 GGCGCC GGC^GCC
SnaBI 2398 TACGTA TAC^GTA
SphI 2827 GCATGC GCATG^C
StuI 4517 AGGCCT AGG^CCT
Tth111I 3986 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3230 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 49 enzymes) #
###################################
AflIII 2441 ACATGT A^C[GA][CT]GT
3397 ACGTGT
AhdI 549 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1197 GACTGGTGGTC
AlwNI 868 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
5099 CAGTTACTG
AspEI 549 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1197 GACTGGTGGTC
AspHI 1449 GAGCAC G[AT]GC[AT]^C
4869 GTGCAC
AvaI 168 CCCGGG C^[CT]CG[GA]G
334 CCCGGG
BglI 96 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
215 GCCCGGCCGGC
BglII 2773 AGATCT A^GATCT
2849 AGATCT
BmyI 1449 GAGCAC G[AGT]GC[ACT]^C
4869 GTGCAC
BsaWI 263 ACCGGA [AT]^CCGG[AT]
1568 ACCGGA
BsiHKAI 1449 GAGCAC G[AT]GC[AT]^C
4869 GTGCAC
BsoBI 168 CCCGGG C^[CT]CG[GA]G
334 CCCGGG
Bsp1286I 1449 GAGCAC G[AGT]GC[ACT]^C
4869 GTGCAC
BspHI 4021 TCATGA T^CATGA
5074 TCATGA
BspMI 4568 ACCTGC ACCTGC(4/8)
674 GCAGGT
BsrFI 220 GCCGGC [GA]^CCGG[CT]
247 GCCGGC
BstAPI 717 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
882 GCAATAGATGC
BstYI 2773 AGATCT [GA]^GATC[CT]
2849 AGATCT
CfoI 213 GCGC GCG^C
258 GCGC
Cfr10I 220 GCCGGC [GA]^CCGG[CT]
247 GCCGGC
DraII 157 GGGTCCC [GA]G^G[ACGT]CC[CT]
4742 AGGTCCC
EagI 218 CGGCCG C^GGCCG
249 CGGCCG
Eam1105I 549 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1197 GACTGGTGGTC
EclXI 218 CGGCCG C^GGCCG
249 CGGCCG
Eco52I 218 CGGCCG C^GGCCG
249 CGGCCG
EcoNI 1609 CCTATAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4520 CCTAAGATAGG
EcoO109I 157 GGGTCCC [GA]G^G[ACGT]CC[CT]
4742 AGGTCCC
HhaI 213 GCGC GCG^C
258 GCGC
HinP1I 213 GCGC G^CGC
258 GCGC
HindIII 3283 AAGCTT A^AGCTT
4238 AAGCTT
HpaI 1892 GTTAAC GTT^AAC
2684 GTTAAC
KspI 153 CCGCGG CCGC^GG
252 CCGCGG
MflI 2773 AGATCT [GA]^GATC[CT]
2849 AGATCT
NaeI 220 GCCGGC GCC^GGC
247 GCCGGC
NgoMIV 220 GCCGGC G^CCGGC
247 GCCGGC
PflMI 896 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1582 CCAAAGACTGG
PpuMI 157 GGGTCCC [GA]G^G[AT]CC[CT]
4742 AGGTCCC
RcaI 4021 TCATGA T^CATGA
5074 TCATGA
SacII 153 CCGCGG CCGC^GG
252 CCGCGG
ScaI 2724 AGTACT AGT^ACT
3390 AGTACT
SmaI 168 CCCGGG CCC^GGG
334 CCCGGG
SwaI 4113 ATTTAAAT ATTT^AAAT
4844 ATTTAAAT
TaqI 482 TCGA T^CGA
1441 TCGA
TthHB8I 482 TCGA T^CGA
1441 TCGA
Van91I 896 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1582 CCAAAGACTGG
XcmI 355 CCAAGCTCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
532 CCAGACTTGGCTTGG
XhoII 2773 AGATCT [GA]^GATC[CT]
2849 AGATCT
XmaCI 168 CCCGGG C^CCGGG
334 CCCGGG
XmaI 168 CCCGGG C^CCGGG
334 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI ApoI AvaII BanI BbsI
BbvI BcnI BfaI BpmI BpuAI
BsaAI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsrDI BsrI
BssKI BstF5I BstNI BstUI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI