##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII BalI BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BlpI Bpu1102I BsaBI BseAI
BsiWI Bsp1407I BspEI BspHI BspMI
BsrGI Bst1107I BstEII BstPI BstZ17I
CelII CpoI DraIII Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
FspI KasI KpnI MamI MluI
MluNI MroI MscI NaeI NarI
NdeI NgoMIV NotI NsiI PacI
PaeR7I PflMI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 113 GACGCC G[GA]^CG[CT]C
AgeI 10 ACCGGT A^CCGGT
ApaI 2028 GGGCCC GGGCC^C
AseI 3494 ATTAAT AT^TAAT
AsnI 3494 ATTAAT AT^TAAT
Asp700I 667 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4183 CCTAGG C^CTAGG
BanI 18 GGTGCC G^G[CT][GA]CC
BbvCI 2853 CCTCAGC CCTCAGC(-5/-2)
BlnI 4183 CCTAGG C^CTAGG
BsaHI 113 GACGCC G[GA]^CG[CT]C
BsaWI 10 ACCGGT [AT]^CCGG[AT]
BsmBI 2073 GAGACG CGTCTC(1/5)
BspDI 1182 ATCGAT AT^CGAT
BsrBI 71 CCGCTC CCGCTC(-3/-3)
BsrFI 10 ACCGGT [GA]^CCGG[CT]
BssSI 1143 CACGAG CACGAG(-5/-1)
BstAPI 1345 GCAAGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 10 ACCGGT [GA]^CCGG[CT]
ClaI 1182 ATCGAT AT^CGAT
DrdI 404 GACCATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 68 CGGCCG C^GGCCG
EclXI 68 CGGCCG C^GGCCG
Eco52I 68 CGGCCG C^GGCCG
EcoNI 199 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 2857 AGCGCC [GA]GCGC^[CT]
HgaI 113 GACGC GACGC(5/10)
Hin1I 113 GACGCC G[GA]^CG[CT]C
HincII 2603 GTTAAC GT[CT]^[GA]AC
HindII 2603 GTTAAC GT[CT]^[GA]AC
HindIII 1920 AAGCTT A^AGCTT
HpaI 2603 GTTAAC GTT^AAC
MfeI 4654 CAATTG C^AATTG
MunI 4654 CAATTG C^AATTG
NheI 2788 GCTAGC G^CTAGC
NruI 1581 TCGCGA TCG^CGA
PinAI 10 ACCGGT A^CCGGT
PshBI 3494 ATTAAT AT^TAAT
SmaI 23 CCCGGG CCC^GGG
SspI 2552 AATATT AAT^ATT
XmaCI 23 CCCGGG C^CCGGG
XmaI 23 CCCGGG C^CCGGG
XmnI 667 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspI 2163 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4621 GACCGTGTC
AvaI 23 CCCGGG C^[CT]CG[GA]G
77 CCCGAG
BglI 234 GCCGCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
763 GCCCCTCTGGC
BsaAI 2114 TACGTG [CT]AC^GT[GA]
4878 TACGTG
BsoBI 23 CCCGGG C^[CT]CG[GA]G
77 CCCGAG
BspLU11I 2715 ACATGT A^CATGT
4012 ACATGT
BsrDI 2286 CATTGC GCAATG(2/0)
2341 CATTGC
BstBI 1250 TTCGAA TT^CGAA
3387 TTCGAA
DraI 3599 TTTAAA TTT^AAA
3687 TTTAAA
DraII 2028 GGGCCCT [GA]G^G[ACGT]CC[CT]
2197 AGGCCCT
EaeI 68 CGGCCG [CT]^GGCC[GA]
3194 CGGCCA
EcoO109I 2028 GGGCCCT [GA]G^G[ACGT]CC[CT]
2197 AGGCCCT
EcoRI 2750 GAATTC G^AATTC
4105 GAATTC
KspI 130 CCGCGG CCGC^GG
1199 CCGCGG
NspV 1250 TTCGAA TT^CGAA
3387 TTCGAA
PflFI 2163 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4621 GACCGTGTC
PvuII 1604 CAGCTG CAG^CTG
1722 CAGCTG
SacI 170 GAGCTC GAGCT^C
479 GAGCTC
SacII 130 CCGCGG CCGC^GG
1199 CCGCGG
SapI 2416 GAAGAGC GCTCTTC(1/4)
172 GCTCTTC
SfuI 1250 TTCGAA TT^CGAA
3387 TTCGAA
Tth111I 2163 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4621 GACCGTGTC
XbaI 2170 TCTAGA T^CTAGA
3753 TCTAGA
XcmI 1578 CCATCGCGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2364 CCAAGCAAGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
Eco81I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII