##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BbrPI BciVI
BfrI BlpI Bpu1102I BsaAI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraIII
DrdI Eam1105I EcoRV EcoT22I FspI
HpaI KpnI MfeI MluI MunI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1437 GACGTC GACGT^C
AccI 2631 GTAGAC GT^[AC][GT]AC
AccIII 2881 TCCGGA T^CCGGA
AflIII 2638 ACGTGT A^C[GA][CT]GT
Aor51HI 925 AGCGCT AGC^GCT
AvrII 3765 CCTAGG C^CTAGG
BamHI 3839 GGATCC G^GATCC
BbeI 1669 GGCGCC GGCGC^C
BbvCI 2935 CCTCAGC CCTCAGC(-5/-2)
BglII 1018 AGATCT A^GATCT
BlnI 3765 CCTAGG C^CTAGG
BsaBI 3208 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2881 TCCGGA [AT]^CCGG[AT]
BseAI 2881 TCCGGA T^CCGGA
BsiEI 4729 CGGCCG CG[GA][CT]^CG
BspEI 2881 TCCGGA T^CCGGA
BsrFI 4545 GCCGGC [GA]^CCGG[CT]
BstEII 3096 GGTGACC G^GT[ACGT]ACC
BstPI 3096 GGTGACC G^GT[ACGT]ACC
Cfr10I 4545 GCCGGC [GA]^CCGG[CT]
EagI 4729 CGGCCG C^GGCCG
EclXI 4729 CGGCCG C^GGCCG
Eco47III 925 AGCGCT AGC^GCT
Eco52I 4729 CGGCCG C^GGCCG
EcoO65I 3096 GGTGACC G^GT[ACGT]ACC
EcoRI 2906 GAATTC G^AATTC
FseI 4544 GGCCGGCC GGCCGG^CC
KasI 1669 GGCGCC G^GCGCC
KspI 1835 CCGCGG CCGC^GG
MamI 3208 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2881 TCCGGA T^CCGGA
NaeI 4545 GCCGGC GCC^GGC
NarI 1669 GGCGCC GG^CGCC
NdeI 1228 CATATG CA^TATG
NgoMIV 4545 GCCGGC G^CCGGC
SacI 3171 GAGCTC GAGCT^C
SacII 1835 CCGCGG CCGC^GG
SexAI 4531 ACCTGGT A^CC[AT]GGT
SfiI 4721 GGCCCTCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1669 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 1142 GAATTT [GA]^AATT[CT]
2906 GAATTC
ApoI 1142 GAATTT [GA]^AATT[CT]
2906 GAATTC
BclI 335 TGATCA T^GATCA
4471 TGATCA
Bsp1407I 3222 TGTACA T^GTACA
4627 TGTACA
BsrDI 1283 CATTGC GCAATG(2/0)
3864 CATTGC
BsrGI 3222 TGTACA T^GTACA
4627 TGTACA
DraI 1025 TTTAAA TTT^AAA
1193 TTTAAA
FbaI 335 TGATCA T^GATCA
4471 TGATCA
HincII 1521 GTTGAC GT[CT]^[GA]AC
4786 GTCAAC
HindII 1521 GTTGAC GT[CT]^[GA]AC
4786 GTCAAC
PaeR7I 1856 CTCGAG C^TCGAG
2851 CTCGAG
PpuMI 5 AGGTCCT [GA]G^G[AT]CC[CT]
844 AGGACCC
ScaI 703 AGTACT AGT^ACT
4249 AGTACT
SspBI 3222 TGTACA T^GTACA
4627 TGTACA
XhoI 1856 CTCGAG C^TCGAG
2851 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI