##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AccII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsiEI
BsiWI BsoBI BspDI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstPI
BstUI BstXI CelII CfoI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FauI FseI FspI HaeII HgaI
HhaI HinP1I HpaI KasI KspI
MluI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1350 GACGTC GACGT^C
Acc65I 4288 GGTACC G^GTACC
AccIII 2018 TCCGGA T^CCGGA
AcyI 1350 GACGTC G[GA]^CG[CT]C
AflII 3515 CTTAAG C^TTAAG
AflIII 3435 ACATGT A^C[GA][CT]GT
AlwNI 3686 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1611 CCTCAGG CC^T[ACGT]AGG
ApaI 2713 GGGCCC GGGCC^C
Asp718I 4288 GGTACC G^GTACC
BbsI 2331 GAAGAC GAAGAC(2/6)
BcnI 1934 CCCGG CC^[GC]GG
BfrI 3515 CTTAAG C^TTAAG
BpuAI 2331 GAAGAC GAAGAC(2/6)
BsaBI 2659 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1350 GACGTC G[GA]^CG[CT]C
BsaWI 2018 TCCGGA [AT]^CCGG[AT]
BseAI 2018 TCCGGA T^CCGGA
BsmBI 435 CGTCTC CGTCTC(1/5)
BspEI 2018 TCCGGA T^CCGGA
BspHI 1326 TCATGA T^CATGA
BspLU11I 3435 ACATGT A^CATGT
BssSI 4750 CACGAG CACGAG(-5/-1)
Bsu36I 1611 CCTCAGG CC^T[ACGT]AGG
DraIII 5079 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1611 CCTCAGG CC^T[ACGT]AGG
EcoNI 3635 CCTGATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1350 GACGTC G[GA]^CG[CT]C
KpnI 4288 GGTACC GGTAC^C
MamI 2659 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2309 CAATTG C^AATTG
MroI 2018 TCCGGA T^CCGGA
MunI 2309 CAATTG C^AATTG
NciI 1934 CCCGG CC^[GC]GG
NcoI 2933 CCATGG C^CATGG
PpuMI 4439 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 1326 TCATGA T^CATGA
SexAI 4291 ACCAGGT A^CC[AT]GGT
SpeI 3906 ACTAGT A^CTAGT
StuI 2489 AGGCCT AGG^CCT
SwaI 1986 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AseI 760 ATTAAT AT^TAAT
4055 ATTAAT
AsnI 760 ATTAAT AT^TAAT
4055 ATTAAT
AspHI 173 GAGCAC G[AT]GC[AT]^C
4747 GAGCAC
BsaI 71 GAGACC GGTCTC(1/5)
5141 GGTCTC
BsgI 2998 CTGCAC GTGCAG(16/14)
3694 GTGCAG
BsiHKAI 173 GAGCAC G[AT]GC[AT]^C
4747 GAGCAC
BspMI 1112 ACCTGC ACCTGC(4/8)
122 GCAGGT
BsrDI 2162 GCAATG GCAATG(2/0)
3956 GCAATG
DsaI 2933 CCATGG C^C[GA][CT]GG
3352 CCGTGG
HapII 1935 CCGG C^CGG
2019 CCGG
HpaII 1935 CCGG C^CGG
2019 CCGG
MspI 1935 CCGG C^CGG
2019 CCGG
PshBI 760 ATTAAT AT^TAAT
4055 ATTAAT
ScaI 1531 AGTACT AGT^ACT
2477 AGTACT
SmlI 3515 CTTAAG C^T[CT][GA]AG
4936 CTCAAG
XcmI 1083 CCACAGGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5040 CCATCATTTTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BalI
BamHI BanI BanII BbvI BclI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstYI BstZ17I
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoRV EcoT14I FbaI Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI