##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspI AviII BamHI
BbeI BbrPI BfrI BglI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I FauI FseI FspI
HaeII HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 929 CGCG CG^CG
AcyI 1338 GACGCC G[GA]^CG[CT]C
AhdI 816 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 816 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1295 CCCGAG C^[CT]CG[GA]G
AvrII 3459 CCTAGG C^CTAGG
BalI 1138 TGGCCA TGG^CCA
BanI 1308 GGCACC G^G[CT][GA]CC
BbvCI 5074 GCTGAGG CCTCAGC(-5/-2)
BcnI 4895 CCGGG CC^[GC]GG
BlnI 3459 CCTAGG C^CTAGG
BsaAI 818 CACGTA [CT]AC^GT[GA]
BsaHI 1338 GACGCC G[GA]^CG[CT]C
BsoBI 1295 CCCGAG C^[CT]CG[GA]G
BstUI 929 CGCG CG^CG
BstXI 3184 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 182 GCGC GCG^C
DraIII 3140 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1138 TGGCCA [CT]^GGCC[GA]
Eam1105I 816 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 150 GAATTC G^AATTC
EcoRV 189 GATATC GAT^ATC
EcoT22I 2918 ATGCAT ATGCA^T
HapII 4895 CCGG C^CGG
HhaI 182 GCGC GCG^C
Hin1I 1338 GACGCC G[GA]^CG[CT]C
HinP1I 182 GCGC G^CGC
HincII 813 GTTGAC GT[CT]^[GA]AC
HindII 813 GTTGAC GT[CT]^[GA]AC
HindIII 4220 AAGCTT A^AGCTT
HpaII 4895 CCGG C^CGG
MfeI 4334 CAATTG C^AATTG
MluNI 1138 TGGCCA TGG^CCA
MscI 1138 TGGCCA TGG^CCA
MspA1I 1379 CAGCTG C[AC]G^C[GT]G
MspI 4895 CCGG C^CGG
MunI 4334 CAATTG C^AATTG
MvnI 929 CGCG CG^CG
NciI 4895 CCGGG CC^[GC]GG
NheI 355 GCTAGC G^CTAGC
NsiI 2918 ATGCAT ATGCA^T
PshAI 1438 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1379 CAGCTG CAG^CTG
StuI 1102 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 1891 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
4266 CAGGGGCTG
BcgI 237 CGATCAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1309 GCACCCGACTCG
BclI 2005 TGATCA T^GATCA
5125 TGATCA
BsaI 3874 GAGACC GGTCTC(1/5)
4996 GAGACC
BsmBI 609 CGTCTC CGTCTC(1/5)
1200 GAGACG
BspLU11I 1392 ACATGT A^CATGT
3196 ACATGT
BsrDI 552 CATTGC GCAATG(2/0)
726 CATTGC
BssSI 1239 CACGAG CACGAG(-5/-1)
1733 CACGAG
DsaI 1431 CCATGG C^C[GA][CT]GG
3797 CCATGG
EcoNI 2368 CCTAAAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4608 CCTGAAACAGG
FbaI 2005 TGATCA T^GATCA
5125 TGATCA
HgaI 1202 GACGC GACGC(5/10)
1338 GACGC
MaeII 819 ACGT A^CGT
2881 ACGT
NcoI 1431 CCATGG C^CATGG
3797 CCATGG
NdeI 452 CATATG CA^TATG
2786 CATATG
PpuMI 1623 AGGTCCT [GA]G^G[AT]CC[CT]
4054 AGGACCT
SacI 1448 GAGCTC GAGCT^C
2033 GAGCTC
ScaI 4135 AGTACT AGT^ACT
4847 AGTACT
SspI 2402 AATATT AAT^ATT
4465 AATATT
XcmI 3640 CCAAATGTAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3677 CCAGCAAGTGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvI BciVI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI