##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AseI AsnI AviII AvrII BamHI
BclI BlnI BsaBI BsiWI Bsp1407I
BspDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
MamI MfeI MluI MunI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 1256 TCCGGA T^CCGGA
AcsI 862 GAATTT [GA]^AATT[CT]
AhdI 3763 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4822 GTGCAC G^TGCAC
ApaLI 4822 GTGCAC G^TGCAC
ApoI 862 GAATTT [GA]^AATT[CT]
Asp700I 1114 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3763 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2962 GGCGCC GGCGC^C
BciVI 2234 GTATCC GTATCC(6/5)
BglII 4208 AGATCT A^GATCT
BseAI 1256 TCCGGA T^CCGGA
BsmBI 3337 GAGACG CGTCTC(1/5)
BspEI 1256 TCCGGA T^CCGGA
BspHI 4389 TCATGA T^CATGA
BspLU11I 4719 ACATGT A^CATGT
BstAPI 690 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 2681 CGGCCG C^GGCCG
Eam1105I 3763 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2681 CGGCCG C^GGCCG
Eco52I 2681 CGGCCG C^GGCCG
HpaI 4860 GTTAAC GTT^AAC
KasI 2962 GGCGCC G^GCGCC
KspI 449 CCGCGG CCGC^GG
MroI 1256 TCCGGA T^CCGGA
NarI 2962 GGCGCC GG^CGCC
NheI 4583 GCTAGC G^CTAGC
RcaI 4389 TCATGA T^CATGA
SacII 449 CCGCGG CCGC^GG
SbfI 2562 CCTGCAGG CCTGCA^GG
ScaI 399 AGTACT AGT^ACT
SexAI 2648 ACCTGGT A^CC[AT]GGT
SfoI 2962 GGCGCC GGC^GCC
SmaI 3121 CCCGGG CCC^GGG
SphI 4142 GCATGC GCATG^C
Sse8387I 2562 CCTGCAGG CCTGCA^GG
SspI 714 AATATT AAT^ATT
XmaCI 3121 CCCGGG C^CCGGG
XmaI 3121 CCCGGG C^CCGGG
XmnI 1114 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflII 631 CTTAAG C^TTAAG
640 CTTAAG
AspI 4228 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5153 GACACCGTC
BcgI 504 CGAGGTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3372 CGAGGCCCCTGC
BfrI 631 CTTAAG C^TTAAG
640 CTTAAG
BglI 109 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3479 GCCTGCGGGGC
BsaI 62 GAGACC GGTCTC(1/5)
5039 GGTCTC
BsaWI 1256 TCCGGA [AT]^CCGG[AT]
1331 ACCGGA
BsiEI 77 CGACCG CG[GA][CT]^CG
2681 CGGCCG
BsmI 4763 GAATGC GAATGC(1/-1)
4993 GAATGC
HindIII 318 AAGCTT A^AGCTT
3754 AAGCTT
NaeI 114 GCCGGC GCC^GGC
499 GCCGGC
NgoMIV 114 GCCGGC G^CCGGC
499 GCCGGC
PflFI 4228 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5153 GACACCGTC
StuI 3308 AGGCCT AGG^CCT
4127 AGGCCT
Tth111I 4228 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5153 GACACCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII