##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
ApaI AscI Asp718I AspEI AspI
BbeI BbvCI BfrI BglI BlpI
Bpu1102I BspHI BssHII CelII CpoI
Eam1105I FseI HindIII KasI KpnI
KspI MluI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmeI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AatII 127 GACGTC GACGT^C
AccIII 940 TCCGGA T^CCGGA
Alw44I 4041 GTGCAC G^TGCAC
AlwNI 288 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 3104 AGCGCT AGC^GCT
ApaLI 4041 GTGCAC G^TGCAC
AvaI 196 CCCGGG C^[CT]CG[GA]G
AviII 3026 TGCGCA TGC^GCA
AvrII 3744 CCTAGG C^CTAGG
BamHI 1303 GGATCC G^GATCC
BbrPI 3364 CACGTG CAC^GTG
BclI 2353 TGATCA T^GATCA
BlnI 3744 CCTAGG C^CTAGG
BsaBI 2103 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 940 TCCGGA [AT]^CCGG[AT]
BseAI 940 TCCGGA T^CCGGA
BseRI 1893 CTCCTC GAGGAG(10/8)
BsiEI 3569 CGGCCG CG[GA][CT]^CG
BsiWI 634 CGTACG C^GTACG
BsmBI 968 GAGACG CGTCTC(1/5)
BsoBI 196 CCCGGG C^[CT]CG[GA]G
BspDI 4335 ATCGAT AT^CGAT
BspEI 940 TCCGGA T^CCGGA
BspLU11I 996 ACATGT A^CATGT
BspMI 4548 ACCTGC ACCTGC(4/8)
BsrBI 2565 CCGCTC CCGCTC(-3/-3)
BsrFI 4099 GCCGGT [GA]^CCGG[CT]
BssSI 4046 CTCGTG CACGAG(-5/-1)
BstAPI 695 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3999 TTCGAA TT^CGAA
BstXI 2024 CCATGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 4099 GCCGGT [GA]^CCGG[CT]
ClaI 4335 ATCGAT AT^CGAT
DraII 590 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 3326 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1746 GACCTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3569 CGGCCG C^GGCCG
EclXI 3569 CGGCCG C^GGCCG
Eco47III 3104 AGCGCT AGC^GCT
Eco52I 3569 CGGCCG C^GGCCG
EcoNI 2334 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 590 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 296 GATATC GAT^ATC
FbaI 2353 TGATCA T^GATCA
FspI 3026 TGCGCA TGC^GCA
HaeII 3104 AGCGCT [GA]GCGC^[CT]
HgaI 1204 GCGTC GACGC(5/10)
MamI 2103 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 940 TCCGGA T^CCGGA
MspA1I 1475 CAGCTG C[AC]G^C[GT]G
NcoI 4636 CCATGG C^CATGG
NdeI 648 CATATG CA^TATG
NheI 1870 GCTAGC G^CTAGC
NspV 3999 TTCGAA TT^CGAA
PmaCI 3364 CACGTG CAC^GTG
PmlI 3364 CACGTG CAC^GTG
PpuMI 590 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 435 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1475 CAGCTG CAG^CTG
SapI 2567 GCTCTTC GCTCTTC(1/4)
SexAI 2638 ACCAGGT A^CC[AT]GGT
SfuI 3999 TTCGAA TT^CGAA
SmaI 196 CCCGGG CCC^GGG
SspI 1558 AATATT AAT^ATT
XmaCI 196 CCCGGG C^CCGGG
XmaI 196 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 1426 CGCG CG^CG
1597 CGCG
AcyI 127 GACGTC G[GA]^CG[CT]C
1203 GGCGTC
AflIII 996 ACATGT A^C[GA][CT]GT
4083 ACACGT
AocI 1522 CCTGAGG CC^T[ACGT]AGG
4590 CCTAAGG
AseI 3 ATTAAT AT^TAAT
4671 ATTAAT
AsnI 3 ATTAAT AT^TAAT
4671 ATTAAT
Asp700I 138 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3249 GAAAACCTTC
BalI 3134 TGGCCA TGG^CCA
4633 TGGCCA
BanI 32 GGCACC G^G[CT][GA]CC
221 GGTGCC
BanII 161 GGGCTC G[GA]GC[CT]^C
4410 GAGCCC
BbsI 1071 GAAGAC GAAGAC(2/6)
2434 GTCTTC
BcgI 1619 CGAGGAAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2917 GCAGGGTGATCG
BpuAI 1071 GAAGAC GAAGAC(2/6)
2434 GTCTTC
BsaAI 3364 CACGTG [CT]AC^GT[GA]
4084 CACGTA
BsaHI 127 GACGTC G[GA]^CG[CT]C
1203 GGCGTC
BsgI 1974 CTGCAC GTGCAG(16/14)
2487 GTGCAG
Bsp1407I 363 TGTACA T^GTACA
2395 TGTACA
BsrGI 363 TGTACA T^GTACA
2395 TGTACA
Bst1107I 1378 GTATAC GTA^TAC
4385 GTATAC
BstEII 3680 GGTGACC G^GT[ACGT]ACC
3889 GGTCACC
BstPI 3680 GGTGACC G^GT[ACGT]ACC
3889 GGTCACC
BstUI 1426 CGCG CG^CG
1597 CGCG
BstZ17I 1378 GTATAC GTA^TAC
4385 GTATAC
Bsu36I 1522 CCTGAGG CC^T[ACGT]AGG
4590 CCTAAGG
CfoI 3027 GCGC GCG^C
3105 GCGC
DsaI 900 CCACGG C^C[GA][CT]GG
4636 CCATGG
Eco81I 1522 CCTGAGG CC^T[ACGT]AGG
4590 CCTAAGG
EcoO65I 3680 GGTGACC G^GT[ACGT]ACC
3889 GGTCACC
EcoRI 142 GAATTC G^AATTC
4077 GAATTC
FauI 2651 CCCGC CCCGC(4/6)
159 GCGGG
HhaI 3027 GCGC GCG^C
3105 GCGC
Hin1I 127 GACGTC G[GA]^CG[CT]C
1203 GGCGTC
HinP1I 3027 GCGC G^CGC
3105 GCGC
HpaI 484 GTTAAC GTT^AAC
1133 GTTAAC
MluNI 3134 TGGCCA TGG^CCA
4633 TGGCCA
MscI 3134 TGGCCA TGG^CCA
4633 TGGCCA
MvnI 1426 CGCG CG^CG
1597 CGCG
NspI 996 ACATGT [GA]CATG^[CT]
1679 ACATGC
PshBI 3 ATTAAT AT^TAAT
4671 ATTAAT
ScaI 1365 AGTACT AGT^ACT
2507 AGTACT
SspBI 363 TGTACA T^GTACA
2395 TGTACA
XmnI 138 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3249 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbvI
BciVI BcnI BfaI BglII BmyI
BpmI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII