##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BbvCI BglI BglII BlnI
BsaAI BsaBI BsiWI BsmBI BsmI
BspLU11I Bst1107I BstBI BstZ17I CpoI
DrdI Eam1105I Eco47III EcoRV FspI
HpaI KpnI MamI MluI MluNI
MscI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AlwNI 320 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 667 ATTAAT AT^TAAT
AsnI 667 ATTAAT AT^TAAT
Asp700I 1441 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4420 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2 GGCGCC GGCGC^C
BcgI 3592 GCAATCCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3217 GTATCC GTATCC(6/5)
BclI 2601 TGATCA T^GATCA
Bsp1407I 3804 TGTACA T^GTACA
BspDI 514 ATCGAT AT^CGAT
BspHI 2637 TCATGA T^CATGA
BsrBI 71 GAGCGG CCGCTC(-3/-3)
BsrGI 3804 TGTACA T^GTACA
BssHII 126 GCGCGC G^CGCGC
BssSI 2495 CACGAG CACGAG(-5/-1)
BstAPI 2745 GCATCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3630 GGTTACC G^GT[ACGT]ACC
BstPI 3630 GGTTACC G^GT[ACGT]ACC
ClaI 514 ATCGAT AT^CGAT
DraII 23 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoNI 116 CCTCGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 23 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3630 GGTTACC G^GT[ACGT]ACC
FbaI 2601 TGATCA T^GATCA
FseI 56 GGCCGGCC GGCCGG^CC
HindIII 4794 AAGCTT A^AGCTT
KasI 2 GGCGCC G^GCGCC
KspI 7 CCGCGG CCGC^GG
MaeII 386 ACGT A^CGT
MfeI 4133 CAATTG C^AATTG
MunI 4133 CAATTG C^AATTG
NarI 2 GGCGCC GG^CGCC
PflFI 4420 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 667 ATTAAT AT^TAAT
RcaI 2637 TCATGA T^CATGA
SacI 2766 GAGCTC GAGCT^C
SacII 7 CCGCGG CCGC^GG
SfoI 2 GGCGCC GGC^GCC
SmaI 45 CCCGGG CCC^GGG
SpeI 4195 ACTAGT A^CTAGT
SphI 2613 GCATGC GCATG^C
SspBI 3804 TGTACA T^GTACA
StuI 1162 AGGCCT AGG^CCT
SwaI 2886 ATTTAAAT ATTT^AAAT
Tth111I 4420 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4735 TCTAGA T^CTAGA
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
XmnI 1441 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 3063 TCCGGA T^CCGGA
4728 TCCGGA
AcyI 2 GGCGCC G[GA]^CG[CT]C
244 GACGCC
AfaI 2086 GTAC GT^AC
3805 GTAC
AocI 1420 CCTGAGG CC^T[ACGT]AGG
1531 CCTGAGG
ApaI 24 GGGCCC GGGCC^C
1869 GGGCCC
BlpI 95 GCTCAGC GC^T[ACGT]AGC
1254 GCTCAGC
Bpu1102I 95 GCTCAGC GC^T[ACGT]AGC
1254 GCTCAGC
BsaHI 2 GGCGCC G[GA]^CG[CT]C
244 GACGCC
BsaWI 3063 TCCGGA [AT]^CCGG[AT]
4728 TCCGGA
BseAI 3063 TCCGGA T^CCGGA
4728 TCCGGA
BseRI 3173 CTCCTC GAGGAG(10/8)
1423 GAGGAG
BsiEI 59 CGGCCG CG[GA][CT]^CG
222 CGGCCG
BspEI 3063 TCCGGA T^CCGGA
4728 TCCGGA
BsrDI 1724 GCAATG GCAATG(2/0)
1823 GCAATG
BsrFI 57 GCCGGC [GA]^CCGG[CT]
147 GCCGGC
BstYI 2334 AGATCC [GA]^GATC[CT]
2352 AGATCC
Bsu36I 1420 CCTGAGG CC^T[ACGT]AGG
1531 CCTGAGG
CelII 95 GCTCAGC GC^T[ACGT]AGC
1254 GCTCAGC
Cfr10I 57 GCCGGC [GA]^CCGG[CT]
147 GCCGGC
EagI 59 CGGCCG C^GGCCG
222 CGGCCG
EclXI 59 CGGCCG C^GGCCG
222 CGGCCG
Eco52I 59 CGGCCG C^GGCCG
222 CGGCCG
Eco81I 1420 CCTGAGG CC^T[ACGT]AGG
1531 CCTGAGG
EcoT22I 713 ATGCAT ATGCA^T
2611 ATGCAT
HaeII 2 GGCGCC [GA]GCGC^[CT]
201 AGCGCC
Hin1I 2 GGCGCC G[GA]^CG[CT]C
244 GACGCC
HincII 868 GTCAAC GT[CT]^[GA]AC
1840 GTTGAC
HindII 868 GTCAAC GT[CT]^[GA]AC
1840 GTTGAC
MflI 2334 AGATCC [GA]^GATC[CT]
2352 AGATCC
MroI 3063 TCCGGA T^CCGGA
4728 TCCGGA
NaeI 57 GCCGGC GCC^GGC
147 GCCGGC
NcoI 1754 CCATGG C^CATGG
2783 CCATGG
NgoMIV 57 GCCGGC G^CCGGC
147 GCCGGC
NsiI 713 ATGCAT ATGCA^T
2611 ATGCAT
RsaI 2086 GTAC GT^AC
3805 GTAC
SapI 2698 GAAGAGC GCTCTTC(1/4)
4723 GCTCTTC
TaqI 515 TCGA T^CGA
2563 TCGA
TthHB8I 515 TCGA T^CGA
2563 TCGA
XhoII 2334 AGATCC [GA]^GATC[CT]
2352 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AflII AluI
Alw44I AlwI ApaLI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII DsaI EaeI EarI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I